Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Antioxidant Activity and Alpha-Glucosidase Inhibitory Activity of Stings of Gleditsia sinensis Extracts

조각자 추출물의 항산화 활성 및 alpha-glucosidase 저해 활성

  • Lee, Jeung-Min (Department of Food Science and Biotechnology, Kyungnam University) ;
  • Park, Jae-Hee (Department of Food and Nutrition, Kyungnam University) ;
  • Chu, Won-Mi (Department of Food and Nutrition, Kyungnam University) ;
  • Yoon, Yi-Mook (Department of Food Science and Biotechnology, Kyungnam University) ;
  • Park, Eun-Ju (Department of Food and Nutrition, Kyungnam University) ;
  • Park, Hae-Ryong (Department of Food Science and Biotechnology, Kyungnam University)
  • 이정민 (경남대학교 식품생명학과) ;
  • 박재희 (경남대학교 식품영양학과) ;
  • 추원미 (경남대학교 식품영양학과) ;
  • 윤이묵 (경남대학교 식품생명학과) ;
  • 박은주 (경남대학교 식품영양학과) ;
  • 박해룡 (경남대학교 식품생명학과)
  • Received : 2010.09.20
  • Accepted : 2010.10.18
  • Published : 2011.01.30
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Abstract

This study was performed to investigate the physiological activities of stings of Gleditsia sinensis extracts. Antioxidant activity was evaluated by measuring total phenolic contents (TPC), comet assay, and 2.2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity (RSA). Anti-diabetic activity was measured by inhibition activities on $\alpha$-glucosidase. Stings of Gleditsia sinensis extracts were prepared by extracting them with methanol and ethanol. The methanolic extracts showed the highest phenol content (1.12 g/100 g gallic acid equivalents). The $\alpha$-glucosidase inhibitory activity of methanol extracts were 17.9% higher, and that of ethanol extracts were 10.3% higher at a concentration of 1 mg/ml. These results indicate that stings of Gleditsia sinensis might be potential candidates as antioxidant and anti-diabetic agents.

본 연구는 항산화 활성 및 항당뇨 효과가 있는 조각자 추출물인 GSM (methanol 추출물)과 GSE (ethanol 추출물)에 대한 연구이다. 추출 용매에 대한 페놀 함량을 측정한 결과 100 g 당 GSM에서는 1.12 g이며, GSE에서는 0.6 g으로 GSM에서 함량이 높은 것으로 나타났다. DPPH 라디칼 소거능의 경우 최대 농도 1,000 ${\mu}g$/ml에서 확인한 결과 GSM에서는 68.8% 그리고 GSE에서는 70.4%로 두 종류 추출물에서 높은 결과를 확인할 수 있었다. DNA 손상 정도를 확인한 comet assay에서도 200 ${\mu}M$ $H_2O_2$에 의해 나타나는 DNA 손상에 대하여 GSM과 GSE는 DNA를 보호하는 활성이 있었다. 항당뇨 효과를 알아보는 $\alpha$-glucosidase 억제 실험에서 GSM은 17.9%, GSE는 10.3%이었으며, 처리시간을 증가하였을 경우에는 그 억제 정도가 GSM의 경우 25%이며, GSE의 경우 20%로 증가하여 항당뇨 효과가 있다는 것을 확인할 수 있었다. 이 결과로부터 당뇨병 치료에 대한 새로운 소재로써 조각자 추출물의 가능성을 확인하였다.

Keywords

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