생명과학회지 (Journal of Life Science)

  • 제21권1호
  • /
  • Pages.62-67
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  • 2011
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  • 1225-9918(pISSN)
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  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
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조각자 추출물의 항산화 활성 및 alpha-glucosidase 저해 활성

Antioxidant Activity and Alpha-Glucosidase Inhibitory Activity of Stings of Gleditsia sinensis Extracts

  • 이정민 (경남대학교 식품생명학과) ;
  • 박재희 (경남대학교 식품영양학과) ;
  • 추원미 (경남대학교 식품영양학과) ;
  • 윤이묵 (경남대학교 식품생명학과) ;
  • 박은주 (경남대학교 식품영양학과) ;
  • 박해룡 (경남대학교 식품생명학과)
  • Lee, Jeung-Min (Department of Food Science and Biotechnology, Kyungnam University) ;
  • Park, Jae-Hee (Department of Food and Nutrition, Kyungnam University) ;
  • Chu, Won-Mi (Department of Food and Nutrition, Kyungnam University) ;
  • Yoon, Yi-Mook (Department of Food Science and Biotechnology, Kyungnam University) ;
  • Park, Eun-Ju (Department of Food and Nutrition, Kyungnam University) ;
  • Park, Hae-Ryong (Department of Food Science and Biotechnology, Kyungnam University)
  • 투고 : 2010.09.20
  • 심사 : 2010.10.18
  • 발행 : 2011.01.30
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초록

본 연구는 항산화 활성 및 항당뇨 효과가 있는 조각자 추출물인 GSM (methanol 추출물)과 GSE (ethanol 추출물)에 대한 연구이다. 추출 용매에 대한 페놀 함량을 측정한 결과 100 g 당 GSM에서는 1.12 g이며, GSE에서는 0.6 g으로 GSM에서 함량이 높은 것으로 나타났다. DPPH 라디칼 소거능의 경우 최대 농도 1,000 ${\mu}g$/ml에서 확인한 결과 GSM에서는 68.8% 그리고 GSE에서는 70.4%로 두 종류 추출물에서 높은 결과를 확인할 수 있었다. DNA 손상 정도를 확인한 comet assay에서도 200 ${\mu}M$ $H_2O_2$에 의해 나타나는 DNA 손상에 대하여 GSM과 GSE는 DNA를 보호하는 활성이 있었다. 항당뇨 효과를 알아보는 $\alpha$-glucosidase 억제 실험에서 GSM은 17.9%, GSE는 10.3%이었으며, 처리시간을 증가하였을 경우에는 그 억제 정도가 GSM의 경우 25%이며, GSE의 경우 20%로 증가하여 항당뇨 효과가 있다는 것을 확인할 수 있었다. 이 결과로부터 당뇨병 치료에 대한 새로운 소재로써 조각자 추출물의 가능성을 확인하였다.

This study was performed to investigate the physiological activities of stings of Gleditsia sinensis extracts. Antioxidant activity was evaluated by measuring total phenolic contents (TPC), comet assay, and 2.2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity (RSA). Anti-diabetic activity was measured by inhibition activities on $\alpha$-glucosidase. Stings of Gleditsia sinensis extracts were prepared by extracting them with methanol and ethanol. The methanolic extracts showed the highest phenol content (1.12 g/100 g gallic acid equivalents). The $\alpha$-glucosidase inhibitory activity of methanol extracts were 17.9% higher, and that of ethanol extracts were 10.3% higher at a concentration of 1 mg/ml. These results indicate that stings of Gleditsia sinensis might be potential candidates as antioxidant and anti-diabetic agents.

키워드

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