DOI QR코드

DOI QR Code

Disposable Electrochemical Immunosensors for the Detection of Herbicide

제초제 검출을 위한 전기화학적 일회용 면역센서

  • Chang, Seung-Cheol (Institute of BioPhysio Sensor Technology (IBST), Pusan National University)
  • 장승철 (부산대학교 바이오피지오센서연구소)
  • Received : 2010.11.02
  • Accepted : 2010.12.15
  • Published : 2011.01.31

Abstract

A disposable electrochemical immunosensor system has been developed for the detection of herbicide in aqueous samples. Disposable screen printed carbon electrodes(SPCE) were used as basic electrodes and an enzyme, horseradish peroxidase (HRP), and anti-herbicide antibodies was immobilised on to the working electrode of SPCE by using avidin-biotin coupling reactions. An herbicide-glucose oxidase conjugates have been used for the competitive immunoreaction with sample herbicides. The enzymatic reaction between the conjugated glucose oxidase and glucose added generates hydrogen peroxide, which was reduced by the peroxidase immobilised. The latter process caused an electrical current change, due to direct re-reduction of peroxidase by a direct electron transfer mechanism, which was measured to determine the herbicides in the sample. The optimal operational condition was found to be: $20\;{\mu}gl-1$ deglycosylated avidin loading to the working electrode and working potential +50 mV vs. Ag/AgCl. The total assay time was 15 min after sample addition. The detection limits for herbicides, atrazine and simazine, were found to be 3 ppb and 10 ppb, respectively.

Keywords

References

  1. S. Schuman and W. Simpson, “A clinical historical overview of pesticide health issues”, Occup. Med., vol. 12, pp. 203-207, 1997.
  2. B. Johnson, “A review of health-based comparative risk assessments in the United States”, Rev. Environ. Health, vol. 15, pp. 273-287, 2000.
  3. P. Hansen and A. Usedom, “New biosensors for environmental analysis”, EXS., vol. 81, pp. 109-120, 1997.
  4. I. Karube, K. Yano, S. Sasaki, Y. Nomura, and K. Ikebukuro, “Biosensors for environmental monitoring”, Ann. NY Acad. Sci., vol. 864, pp. 23-36, 1998. https://doi.org/10.1111/j.1749-6632.1998.tb10285.x
  5. A. Warsinke, A. Benkert, and F. W. Scheller, “Electrochemical immunoassays”, Fresenius J. Anal. Chem., vol. 366, pp. 622-634, 2000. https://doi.org/10.1007/s002160051557
  6. R. Scheller, U. Wollenberger, A. Warsinke and F. Lisdat, “Research and development in biosensors”, Curr. Opin. Biotechnol., vol. 12, pp. 35-40, 2001. https://doi.org/10.1016/S0958-1669(00)00169-5
  7. D. Park, Y. Shim and S. Chang, “Tri-enzyme modified electrochemical biosensor for paracetamol detection”, J. Kor. Sensors Soc., vol. 17, no. 1, pp. 29-34, 2008. https://doi.org/10.5369/JSST.2008.17.1.029
  8. S. Kroger, P. F. Turner, K. Mosbach, and K. Haupt, “Imprinted polymer-based sensor system for herbicides using differential-pulse voltammetry on screen-printed electrodes”, Anal. Chem., vol. 71, pp. 3698-3702, 1999. https://doi.org/10.1021/ac9811827
  9. T. Neufeld, I. Eshkenazi, E. Cohen, and J. Rishpon, “A micro flow injection electrochemical biosensor for organophosphorus pesticides”, Biosens. Bioelectr., vol. 15, pp. 323-329, 2000. https://doi.org/10.1016/S0956-5663(00)00073-7
  10. S. Chang, K. Rawson, and C. McNeil, “Disposable tyrosinase-peroxidase bi-enzyme sensor for amperometric detection of phenols”, Biosens. Bioelectr, vol. 17, pp. 1015-1023, 2002. https://doi.org/10.1016/S0956-5663(02)00094-5
  11. S. Chang and D. Park, “Disposable in-field electrochemical potable sensor system for free available chlorine (FAC) detection”, J. Kor. Sensors Soc., vol. 16, no. 6, pp. 449-456, 2007. https://doi.org/10.5369/JSST.2007.16.6.449
  12. B. Dzantiev, E. Yazynina, A. Zherdev, Y. Plekhanova, A. Reshetilov, S. Chang, and C. McNeil, "Determination of the herbicide chlorsulfuron by amperometric sensor based on separation-free bienzyme immunoassay". Sens. Actuat. B., vol. 98, pp. 254-261, 2004. https://doi.org/10.1016/j.snb.2003.10.021
  13. Z. Marshall and Rhodes. C. T., "Modification of trinder's method for the assay of salicylates in biological fluids", Clinic. Chimica Acta, vol. 54, pp. 135-136, 1974. https://doi.org/10.1016/0009-8981(74)90053-9
  14. AOAC, Association of Agricultural Chemists official method 974.27, “Official methods of analysis of AOAC international”, 18thEd., AOAC, Arlington, USA, 2010.
  15. 80/778/EEC, Council directive relating to the quality of water intended for human consumption, Official J. of the E.C., L 229/11-23, 1980.

Cited by

  1. Development of Disposable Enzyme-linked Immunosensor Strip Platform vol.20, pp.6, 2011, https://doi.org/10.5369/JSST.2011.20.6.400