Annals of Laboratory Medicine

Korean Society for Laboratory Medicine (대한진단검사의학회)
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Comparison of an Automated Repetitive Sequence-based PCR Microbial Typing System with IS6110-Restriction Fragment Length Polymorphism for Epidemiologic Investigation of Clinical Mycobacterium tuberculosis Isolates in Korea

  • Jang, Mi-Hee (Department of Laboratory Medicine, School of Medicine, Pusan National University) ;
  • Go, Hee-Jang (Department of Laboratory Medicine, School of Medicine, Pusan National University) ;
  • Shin, Bo-Moon (Department of Laboratory Medicine, Sanggye Paik Hospital, Inje University) ;
  • Lee, Seon-Ho (Department of Laboratory Medicine, University of Ulsan College of Medicine Ulsan University Hospital) ;
  • Kim, Sung-Ryul (Department of Laboratory Medicine, University of Ulsan College of Medicine Ulsan University Hospital) ;
  • Chang, Chul-Hun L. (Department of Laboratory Medicine, School of Medicine, Pusan National University) ;
  • Kim, Jeong-Man (Department of Laboratory Medicine, Dong-A University College of Medicine)
  • Published : 2011.10.30
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Abstract

Tuberculosis remains a severe public health problem worldwide. Presently, genotyping is used for conducting epidemiologic and clinical studies on tuberculosis cases. We evaluated the efficacy of the repetitive sequence-based PCR (rep-PCR)-based DiversiLabTM system (bioM$\acute{e}$rieux, France) over the IS6110-restriction fragment length polymorphism analysis for detecting Mycobacterium tuberculosis. In all, 89 clinical M. tuberculosis isolates collected nationwide from Korea were used. The DiversiLab system allocated the 89 isolates to 8 groups with 1 unique isolate when a similarity level of 95% was applied. Seventy-six isolates of the Beijing family and 13 isolates of non-Beijing family strains were irregularly distributed regardless of rep-PCR groups. The DiversiLab system generated a rapid, sensitive, and standardized result. It can be used to conduct molecular epidemiologic studies to identify clinical M. tuberculosis isolates in Korea.

Keywords

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