Korean Journal of Fisheries and Aquatic Sciences (한국수산과학회지)

The Korean Society of Fisheries and Aquatic Science (한국수산과학회)
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Accumulation and Depletion of Melamine Through Experimental Feeding in Catfish Silurus asotus

메기(Silurus asotus)에 투여한 멜라민의 체내함량 변화

  • Kim, Poong-Ho (Southeast Sea Fisheries Research Institute, National Fisheries Research and Development Institute) ;
  • Jo, Mi-Ra (Southwest Sea Fisheries Research Institute, National Fisheries Research and Development Institute) ;
  • Lee, Hee-Jung (Southeast Sea Fisheries Research Institute, National Fisheries Research and Development Institute) ;
  • Kim, Kyoung-Duck (Aquafeed Research Center, National Fisheries Research and Development Institute) ;
  • Ha, Kwang-Soo (Southeast Sea Fisheries Research Institute, National Fisheries Research and Development Institute) ;
  • Yoo, Hyun-Duk (Southeast Sea Fisheries Research Institute, National Fisheries Research and Development Institute) ;
  • Lee, Hong-Sik (Food and Safety Research Division, National Fisheries Research and Development Institute) ;
  • Lee, Doo-Seog (Food and Safety Research Division, National Fisheries Research and Development Institute) ;
  • Yoon, Ho-Dong (Food and Safety Research Division, National Fisheries Research and Development Institute)
  • 김풍호 (국립수산과학원 남동해수산연구소) ;
  • 조미라 (국립수산과학원 남서해수산연구소) ;
  • 이희정 (국립수산과학원 남동해수산연구소) ;
  • 김경덕 (국립수산과학원 사료연구센터) ;
  • 하광수 (국립수산과학원 남동해수산연구소) ;
  • 유현덕 (국립수산과학원 남동해수산연구소) ;
  • 유홍식 (국립수산과학원 식품안전과) ;
  • 이두석 (국립수산과학원 식품안전과) ;
  • 윤호동 (국립수산과학원 식품안전과)
  • Received : 2011.09.07
  • Accepted : 2011.10.31
  • Published : 2011.12.30
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Abstract

In 2007, pet food contaminated with melamine caused hundreds of dogs and cats to develop renal failure all over the world. In 2008, over 294,000 infants consumed infant formula and developed kidney stones in China. Further investigation revealed that fish feed also contained melamine; this raised concerns about melamine residues in edible fish tissues, which could have caused the urinary tract stone epidemic. In Korea, catfish fed with assorted feed that included cuttlefish organs that contained melamine developed whitening syndrome and fell dead in some populations in 2008. This event raised suspicions about the toxicity of melamine and all feeds containing melamine were immediately recalled. In this study, we investigated the rates of melamine accumulation and depletion in muscle and viscera of catfish to propose proper withdrawal periods. One group of catfish was fed a commercially available diet that contained 30, 100 and 300 mg melamine per kg diet for 14 days. To investigate residual melamine contents in muscle and viscera, other experimental groups were fed a melamine free diet after being fed melamine for 7 days. The residual amount of melamine was analyzed by LC-MS/MS. The melamine concentration in muscle was estimated to be 3.7 mg/kg after 6 days of feeding with a diet containing 300 mg melamine/kg. After 2 days of culture with a melamine free diet, the residual melamine was depleted and the concentration had decreased from 1.15 mg/kg to 0.19 mg/kg in the muscle of catfish fed a diet containing 300 mg melamine/kg for 7 days. The residual amount of melamine was reduced to 0.03 mg/kg in muscle after 7 days of culture with a melamine free diet and was undetectable after a prolonged culture period of 14 days. Catfish tend to excrete melamine rapidly after oral administration and changes in body color were not observed during the short dosing period.

Keywords

References

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