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Temperature-dependent Index of Mitotic Interval (τ0) for Chromosome Manipulation in Korean Rose Bitterling Rhodeus uyekii

  • Kim, Bong-Seok (Biotechnology Research Division, National Fisheries Research and Development Institute) ;
  • Lim, Sang-Gu (Inland Aquaculture Research Center, National Fisheries Research and Development Institute) ;
  • Gil, Hyun-Woo (Division of Marine Environment and Bioscience, Korea Maritime University) ;
  • Park, In-Seok (Division of Marine Environment and Bioscience, Korea Maritime University)
  • Received : 2011.10.12
  • Accepted : 2011.11.09
  • Published : 2011.12.31

Abstract

Eggs of Korean rose bitterling Rhodeus uyekii were collected and fertilized to observe temperature-related cleavage rates and mitotic intervals (${\tau}_0$). As the water temperature was increased, the slope of first cleavage frequency with elapsed time after fertilization increased, and approximately 30% of fertilized eggs reached first cleavage frequency at every 15 min. At higher temperatures, eggs developed faster and underwent further identical developmental processes. There were strong, negative correlations between ${\tau}_0$ and water temperatures at all temperatures studied (Y = -1.225X + 70.05, $r^2=0.988$, where Y is ${\tau}_0$ and X is temperature).

Keywords

References

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