Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Amyloid-β Levels in Mice Hippocampus According to the ALDH2 Enzyme Activity followed Ethanol Exposure for 8-Weeks

ALDH2 효소 활성과 8주간 에탄올 노출에 따른 해마조직의 아밀로이드 베타 발현

  • Moon, Sun-In (Department of Preventive Medicine, College of Medicine and Medical Research Institute, Chungbuk National University) ;
  • Eom, Sang-Yong (Department of Preventive Medicine, College of Medicine and Medical Research Institute, Chungbuk National University) ;
  • Yim, Dong-Hyuk (Department of Preventive Medicine, College of Medicine and Medical Research Institute, Chungbuk National University) ;
  • Song, Sun-Ho (Department of Preventive Medicine, College of Medicine and Medical Research Institute, Chungbuk National University) ;
  • Kim, Yong-Dae (Department of Preventive Medicine, College of Medicine and Medical Research Institute, Chungbuk National University) ;
  • Kim, Heon (Department of Preventive Medicine, College of Medicine and Medical Research Institute, Chungbuk National University)
  • 문선인 (충북대학교 의과대학 예방의학교실 및 의학연구소) ;
  • 엄상용 (충북대학교 의과대학 예방의학교실 및 의학연구소) ;
  • 임동혁 (충북대학교 의과대학 예방의학교실 및 의학연구소) ;
  • 송선호 (충북대학교 의과대학 예방의학교실 및 의학연구소) ;
  • 김용대 (충북대학교 의과대학 예방의학교실 및 의학연구소) ;
  • 김헌 (충북대학교 의과대학 예방의학교실 및 의학연구소)
  • Received : 2011.09.06
  • Accepted : 2011.11.16
  • Published : 2011.11.30
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Abstract

Alzheimer's disease (AD) is a progressive neurodegenerative disease, resulting in the loss of cognitive function. Mitochondrial aldehyde dehydrogenase (ALDH2) has been proposed to be a risk factor for the development of AD, but there is still controversy about that. In this study, we demonstrated the role of ALDH2 enzyme activity on amyloid-beta (A${\beta}$) and nuclear factor kappa B (NF-${\kappa}B$) expression in mice brain following ethanol exposure for 8 weeks. Five male Aldh2 (+/+) and Aldh2 (-/-) mice, 8 weeks-old of age (C57BL/6J strain), in each group were exposed to ethanol for 8 weeks (2 g/kg wt./day) using gavage. Those in the control groups received 0.9% saline alone. Results showed a difference in expression level of A${\beta}$ in the hippocampus after ethanol exposure according to the ALDH2 enzyme activity (p<0.05), but not in the level of NF-${\kappa}B$). Our results suggest a possibility that ALDH2 enzyme activity may be an important role in the development of AD.

알츠하이머 질환은 인지능력과 행동능력 두 가지에 모두 영향을 미치는 진행적 노인성 치매증의 일종으로 정확한 발병기전은 아직 알려져 있지 않으나 환경적 요인 및 유전적 요인이 모두 중요한 위험인자로 알려져 있다. 본 연구에서는 8주간 에탄올에 노출된 Aldh2 knockout mouse 뇌조직을 분리하여 알츠하이머 질환의 지표로 잘 알려진 아밀로이드 베타와 NF-kB 발현을 평가하고 이러한 변화가 ALDH2 효소의 활성에 따라 어떻게 달라지는 지 비교하였다. 그 결과, 8주간 에탄올을 경구 투여한 마우스에서 ALDH2 효소의 활성에 따른 NF-kB 농도의 차이는 확인할 수 없었으나, ALDH2 효소 활성은 알코올 노출에 의한 해마조직의 아밀로이드 베타 축적에서 중요한 영향을 미치는 것으로 나타났다(p<0.05). 이러한 결과는 ALDH2 효소가 결핍된 사람이 결핍되지 않은 사람에 비해 알코올에 의한 알츠하이머 질환의 발생에 보다 민감할 수 있음을 시사한다.

Keywords

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