Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
권호 표지
DOI QR코드

DOI QR Code

Plant Growth-Promoting Activity of Endophytic Fungi Isolated from the Roots of Native Plants in Dokdo Islands

독도 자생식물의 뿌리로부터 분리된 내생균의 식물생장촉진 활성

  • You, Young-Hyun (Department of Life Sciences and Biotechnology, Kyungpook National University) ;
  • Yoon, Hyeok-Jun (Department of Life Sciences and Biotechnology, Kyungpook National University) ;
  • Woo, Ju-Ri (Department of Life Sciences and Biotechnology, Kyungpook National University) ;
  • Seo, Yeong-Gyo (Department of Life Sciences and Biotechnology, Kyungpook National University) ;
  • Kim, Mi-Ae (Department of Life Sciences and Biotechnology, Kyungpook National University) ;
  • Choo, Yeon-Sik (Department of Biology, College of National Sciences, Kyungpook National University) ;
  • Kim, Jong-Guk (Department of Life Sciences and Biotechnology, Kyungpook National University)
  • Received : 2011.10.19
  • Accepted : 2011.11.11
  • Published : 2011.11.30
Download PDF
⟨ Previous Next ⟩

Abstract

Endophytic fungal strains were isolated from the roots of six species plants in the Dokdo islands. Native plant samples, such as Artemisia japonica, Chenopodium album and Solanum nigrum were isolated from Dongdo, and those such as Cyrtomium falcatum, Dianthus longicalyx and Tetragonia tetragonoides were isolated from Seodo. In total, thirty two fungal strains were isolated from these native plants. To identify the fungal strains, polymerase chain reaction (PCR) amplification of internal transcribed spacer (ITS: containing ITS1, 5.8s and ITS2 region) regions was done with universal primers ITS1 and ITS4. Endophytic fungi of four species were isolated from A. japonica, eight species from C. album, three species from S. nigrum, three species from C. falcatum, three species from D. longicalyx and eleven species from T. tetragonoides. Culture filtrates (CF) of isolated endophytic fungi were used to treatwaito-c rice seedlings to test plant growth-promoting activity. As a result of bioassay, Ca-5-2-2 strain isolated from C. album expressed highest plant growth-promotion activity. Of all the endophytic fungi isolated, Penicillium sp., Fusarium sp. and Aspergillus sp. were the most abundantly distributed fungal strains in the six plants used in this study.

독도에 자생하고 있는 6 종류의 식물뿌리로부터 내생균을 분리하였다. 동도에서 갯제비쑥, 명아주, 까마중과 서도에서 도깨비쇠고비, 술패랭이 그리고 번행초를 연구재료로 사용하였다. 총 32 종의 내생균을 분리하였고, universal primers ITS1과 ITS4를 사용하여 ITS 영역을 PCR로 증폭하여 동정하였다. 염기서열 분석결과, 6 종류의 식물의 뿌리에서 모두 32종류의 다양한 내생균류를 분리 및 동정 할 수 있었다. 독도 자생식물인 갯제비쑥 4종, 명아주 8종, 까마중 3종, 도깨비쇠고비 3종, 술패랭이 3종, 번행초 11종의 내생균이 분리되었다. 내생균들의 배양여액은 식물생장촉진활성을 확인하기 위하여 난장이벼의 유묘에 처리되었다. 그 결과, 명아주에서 분리된 Ca-5-2-2 균주가 우수하게 식물생장촉진활성을 나타내었다. 그리고 독도에 자생하는 6종류의 식물에서 Penicillium sp.와 Fusarium sp. 그리고 Aspergillus sp.의 내생균이 가장 많이 존재함을 알 수 있었다.

Keywords

References

  1. Clay, K. and J. Holah. 1999. Fungal endophyte symbiosis and plant diversity in successional fields. Science 285, 1742-1744. https://doi.org/10.1126/science.285.5434.1742
  2. Ham, M. S., Y. M. Park, H. R. Sung, M. Sumayo, C. M. Ryu, S. H. Park, and S. Y. Ghim. 2009. Characterization of rhizobacteria isolated from family Solanaceae plants in Dokdo island. Korean J. Microbiol. Biotechnol. 37, 110-117.
  3. Hasan, H. A. 2002. Gibberellin and auxin-production by plant root-fungi and their biosynthesis under salinity-calcium interaction. Acta Microbiol. Immunol. Hung. 49, 105-118. https://doi.org/10.1556/AMicr.49.2002.1.11
  4. Hwang, J. S., H. Y. You, J. J. Bae, S. A. Khan, J. G. Kim, and Y. S. Choo. 2011. Effects of endophytic fungal secondary metabolites on the growth and physiological response of Carex kobomugi Ohwi. J. Coastal Res. 27, 544-548. https://doi.org/10.2112/JCOASTRES-D-10-00090.1
  5. Jeon, Y. G. 2005. The regional geomorphology of Dokdo (Volcanic Island). Korean J. Reg. Geol. 11, 19-28.
  6. Kim, M. H., Y. J. Oh, C. S. Kim, M. S. Han, J. T. Lee, and Y. E. Na. 2007. The flora and vegetation distribution in Dokdo. Korean J. Environ. Agric. 26, 85-93. https://doi.org/10.5338/KJEA.2007.26.1.085
  7. Khan, S. A., M. Hamayun, S. O. Rim, I. J. Lee, J. C. Seu, Y. S. Choo, I. N. J, S. D. Kim, I. K. Lee, and J. G. Kim. 2008. Isolation of endophytic fungi capable of plant growth promotion from monocots inhibited in the coastal sand dunes of Korea. J. Life Sci. 18, 1355-1359. https://doi.org/10.5352/JLS.2008.18.10.1355
  8. Khan, S. A., M. Hamayun, H. Y. Kim, H. J. Yoon, I. J. Lee, and J. G. Kim. 2009. Gibberellin production and plant growth promotion by a newly isolated strain of Gliomastix murorum. World J. Microbiol. Biotechnol. 25, 829-833. https://doi.org/10.1007/s11274-009-9981-x
  9. Khan, S. A., M. Hamayun, H. J. Yoon, H. Y. Kim, S. J. Suh, S. K. Hwang, J. M. Kim, I. J. Lee, Y. S. Choo, U. H. Yoon, W. S. Kong, B. M. Lee, and J. G. Kim. 2008. Plant growth promotion and Penicillium citrinum. BMC Microbiol. 8, 231. https://doi.org/10.1186/1471-2180-8-231
  10. Khan, S. A., M. Hamayun, H. Y. Kim, H. J. Yoon, J. C. Seo, Y. S. Choo, Y. J. Kil, J. K. Eo, and A. H. Eom. 2009. Molecular identification and diveristy of endophytic fungi isolated from Pinus densiflora in Boeun, Korea. Korean J. Mycol. 37, 130-133. https://doi.org/10.4489/KJM.2009.37.2.130
  11. Khan, S. A., M. Hamayun, H. Y. Kim, H. J. Yoon, J. C. Seo, Y. S. Choo, I. J. Lee, S. D. Kim, I. K. Rhee, and J. G. Kim. 2009. A new strain of Arthrinium phaeospermum isolated from Carex kobomugi Ohwi is capable of gibberellin production. Biotechnol. Lett. 31, 283-287. https://doi.org/10.1007/s10529-008-9862-7
  12. Lee, D. B. and S. U. Joo. 1958. Reinvestigation of the flora of the Dagelet Island. Humani. Sci. Nat. Sci. 3, 223-296.
  13. Lee, D. H., S. H. Cho, and J. H. Pak. 2007. The analysis of vascular plant species composition in Dok-do island. Korean J. Plant Taxon. 37, 545-563.
  14. Park, S. J., I. G. Song, S. J. Park, and D. O. Lim. 2010. The Flora and Vegetation of Dokdo Island in Ulleung-gun, Gyeongsanbuk-do. Korean J. Env. Eco. 24, 264-278.
  15. Research Institute for Ullengdo & Dokdo Islands. 2008. pp. 166-221, The plant of Dokdo island. Nature of Dokdo island. Kyungpook National University Press. Daegu. Korea.
  16. Seo, S. T., K. H. Kim, M. J. Kim, J. S. Hong, J. H. Park, and S. C. Shin. 2009. Diversity of fungal endophytes from Pinus koraiensis leaves in Korea. Korean J. Mycol. 37, 108-110. https://doi.org/10.4489/KJM.2009.37.1.108
  17. Sun, B. Y., M. R. Sul, J. A. Im, C. H. Kim, and T. J. Kim. 2002. Evolution of endemic vascular plants of Ulleungdo and Dokdo in Korea - floristic and cytotaxonomic characteristics of vascular flora of Dokdo. Korean J. Plant Taxon. 32, 143-158.
  18. Shin, H. T., S. J. Park, K. H. Kang, and J. H. Yoo. 2004. The establishment of conservation area and conservation strategy in Ulnung Island. Korean J. Environ. Eco. 18, 221-230.
  19. Vazquez, M. M., S. Cesar, R. Azcon, and J. M. Barea. 2000. Interaction between arbuscular mycorrhizal fungi and other microbial inoculants (Azospirillum, Pseudomonas, Trichoderma) and their effects on microbial population and enzyme activities in the rhizosphere of maize plants. Appl. Soil Ecol. 15, 261-272. https://doi.org/10.1016/S0929-1393(00)00075-5
  20. Yamada, A., O. Takeo, D. Yosuke, and O. Masatake. 2001. Isolation of Tricholoma matsutake and T. bakamatsutake cultures from field-collected ectomycorrhizas. Mycoscience 42, 43-50. https://doi.org/10.1007/BF02463974
  21. Yoon, J. H., S. Y. Kang, M. H. Lee, and T. K. Oh. 2005. Virgibacillus dokdonensis sp. nov., isolated from a Korean island, Dokdo, located at the edge of the East Sea in Korean. Int. J. Syst. Evol. Microbiol. 51, 1079-1086. https://doi.org/10.1099/00207713-51-3-1079
  22. You, Y. H., H. Yoon, G. S. Lee, J. R. Woo, J. H. Shin, I. J. Lee, S. O. Rim, Y. S. Choo, and J. G. Kim. 2011. Diversity and plant growth-promotion of endophytic fungi isolated from the roots of plants in Dokdo islands. J. Life Sci. 21, 992-996 https://doi.org/10.5352/JLS.2011.21.7.992

Cited by

  1. Plant Growth-Promoting Activity and Genetic Diversity of Endophytic Fungi Isolated from Native Plants in Dokdo Islands for Restoration of a Coastal Ecosystem vol.23, pp.1, 2013, https://doi.org/10.5352/JLS.2013.23.1.95
  2. Metagenomic analysis of soil fungal communities on Ulleungdo and Dokdo Islands vol.61, pp.3, 2015, https://doi.org/10.2323/jgam.61.67
  3. Characterization and phylogenetic analysis of halophilic bacteria isolated from rhizosphere soils of coastal plants in Dokdo islands vol.51, pp.1, 2015, https://doi.org/10.7845/kjm.2015.5008