Biofilter performance and bacterial activity under intermittent and fluctuant xylene loads

간헐적이고 불규칙적인 부하 조건에서 바이오필터의 Xylene 제거 성능 및 세균 활성

  • Lee, Eun-Hee (Department of Environmental Science and Engineering, Ewha Womans University) ;
  • Choi, Sun Ah (Department of Environmental Science and Engineering, Ewha Womans University) ;
  • Cho, Kyung-Suk (Department of Environmental Science and Engineering, Ewha Womans University)
  • 이은희 (이화여자대학교 환경공학과) ;
  • 최선아 (이화여자대학교 환경공학과) ;
  • 조경숙 (이화여자대학교 환경공학과)
  • Received : 2011.02.24
  • Accepted : 2011.03.19
  • Published : 2011.03.31

Abstract

The effects of different transient loading conditions on xylene removability and bacterial activity were evaluated in the polyurethane (PU) biofilter inoculated with Rhodococcus sp. EH831: BF1 (the control) was operated under continuous and constant loading ($200{\pm}50ppm$); BF2 under continuous and constant loading after a 2-week long-term shutdown; BF3 under discontinuous (8 h on/16 h off on weekdays and a 2 day-shutdown at weekends); and constant loading BF4 under discontinuous and high loading ($700{\pm}300ppm$); and BF5 under continuous and fluctuating loading (high loading for 8 h and low loading ($75{\pm}25ppm$) for 16 h on weekdays and low loading at weekends). Xylene removal efficiencies in the BF1, BF2, BF3, and BF5 were ranged 83-89%, indicating that 2-week long-term shutdown, intermittent or fluctuant loading condition did not significantly influence the biofilter performance. However, discontinuous and high load condition (BF4) caused to deteriorate xylene removability to 52%. Rhodococcus sp. EH831 could be maintained at $10^9{\sim}10^{10}CFU/g-dry$ PU under 5 kinds of loading conditions. The result of polymerase chain reaction-terminal-restriction fragment length polymorphism showed that there was no significant change in bacterial community structures by different loading conditions.

Keywords

References

  1. Cho, E., Galera, M. M., Lorenzana, A., and Chung, W. J. (2009). Ethylbenzene, o-xylene, and BTEX removal by Sphingomonas sp. D3K1 in rock wool-compost biofilters., Environmental Engineering and Science, 26, 45-52. https://doi.org/10.1089/ees.2007.0144
  2. Cho, W., Lee, E. H., Shim, E. H., Kim, J., Ryu, H. W., and Cho, K. S. (2005). Bacterial communities of biofilms sampled from seepage groundwater contaminated with petroleum oil, Journal of Microbiology and Biotechnoloy, 15, 952-964.
  3. Devinny, J. S., Deshusses, M. A., and Webster, T. S. (1999). Biofiltration for air pollution control, Lewis Publishers, NY, USA.
  4. Egert, M. and Friedrich, M. W. (2003). Formation of pseudo-terminal restriction fragments, a PCR related bias affection terminal restriction fragments length polymorphism analysis of microbial community structure, Applied and Environmental Microbiology, 69, 2555-2562. https://doi.org/10.1128/AEM.69.5.2555-2562.2003
  5. Elmrini, H., Bredin, N., Shareefdeen, Z., and Heitz, M. (2004). Biofiltration of xylene emissions: bioreactor response to variations in the pollutant inlet concentration and gas flow rate, Chemical Engineering Journal, 100, 149-158. https://doi.org/10.1016/j.cej.2004.01.030
  6. Forsyth, C. S. and Faust, R. A. (1994). Toxicity summary for xylene, Center for integrated Risk Assessment (http://cira.ornl.gov/).
  7. Jeong, E., Hirai, M., and Shoda, M. (2008). Removal of o-xylene using biofilter inoculated with Rhodococcus sp. BTO62, Journal of Hazardous Materials, 152, 140-147. https://doi.org/10.1016/j.jhazmat.2007.06.078
  8. Jiang, X., Yan, R., and Tay, J. H. (2009). Transient-state biodegradation behavior of a horizontal biotrickling filter in co-treating gaseous $H_{2}S$ and $NH_{3}$, Applied Microbiology and Biotechnology, 81, 969-975. https://doi.org/10.1007/s00253-008-1759-9
  9. Kim, D. and Sorial, G. A. (2007). Role of biological activity and biomass distribution in air biofilter performance, Chemosphere, 66, 1758-1764. https://doi.org/10.1016/j.chemosphere.2006.06.069
  10. Kim, D., Cai, Z., and Sorial, G. (2005). Behavior of trickle-bed air biofilter for toluene removal: effect of non-use periods, Environmental Progress, 24, 155-161. https://doi.org/10.1002/ep.10079
  11. Kown, H. H., Lee, E. Y., Cho, K. S., and Ryu, H. W. (2003). Benzene biodegradation using the polyurethane biofilter immobilized with Stenotrophomonas maltophilia T3-c, Journal of Microbiology and Biotechnology, 13, 70-76.
  12. Lee, E. H. and Cho, K. S. (2009). Effect of substrate interaction on the degradation of methyl tert-butyl ether, benzene, toluene, ethylbenzene, and xylene by Rhodococcus sp., Journal of Hazardous Materials, 167, 669-674. https://doi.org/10.1016/j.jhazmat.2009.01.035
  13. Lee, E. H., Kim, J., Cho, K. S., Ahn, Y. G., and Hwang, G. S. (2010). Degradation of hexane and other recalcitrant hydrocarbons by a novel isolate, Rhodococcus sp. EH831, Environmental Science and Pollution Research, 17, 64-77. https://doi.org/10.1007/s11356-009-0238-x
  14. Lee, E. H., Ryu, H. W., and Cho, K. S. (2009). Removal of benzene and toluene in polyurethane biofilter immobilized with Rhodococcus sp. EH831 under transient loading, Bioresource Technology, 100, 5656-5663. https://doi.org/10.1016/j.biortech.2009.06.036
  15. Mathur, A. K. and Majumder, C. B. (2008). Biofiltration and kinetic aspects of a biotrickling filter for the removal of paint solvent mixture laden air stream, Journal of Hazardous Materials, 152, 1027-1036. https://doi.org/10.1016/j.jhazmat.2007.07.112
  16. McCune, B. and Mefford, M. J. (1999). Multivariate analysis of ecological data, version 4, MjM Software Design, Gleneden Beach, Oregon, USA.
  17. Moussavi, G. and Mohseni, M. (2007). Using UV pretreatment to enhance biofiltration of mixtures of aromatic VOCs, Journal of Hazardous Materials, 144, 59-66. https://doi.org/10.1016/j.jhazmat.2006.09.086
  18. Muyzer, G., Teske, A., Wirsen, C. O., and Jannasch, H. W. (1995). Phylogenetic relationships of Thimicrospira species and their identification in deep-sea hydrothermal vent samples by denaturing gradient gel electrophoresis of 16S rDNA fragments, Archives of Microbiology, 164, 165-172. https://doi.org/10.1007/BF02529967
  19. Qi, B. and Moe, W. M. (2006). Performance of low pH biofilters treating a paint solvent mixture: Continuous and intermittent loading, Journal of Hazardous Materials, B135, 303-310.
  20. Raghuvanshi, S. and Babu, B. V. (2009). Exprimental studies and kinetic modeling for removal of methyl ethyl ketone using biofiltration, Bioresource Technology, 100, 3855-3866. https://doi.org/10.1016/j.biortech.2009.03.025
  21. Rene, E. R., Veiga, M. C., and Kennes, C. (2009). Performance of a biofilter for the removal of high concentrations of styrene under steady and non-steady state conditions, Journal of Hazardous Materials, 168, 282-290. https://doi.org/10.1016/j.jhazmat.2009.02.032
  22. Santos, S., Jones, K., Abdul, R., Boswell, J., and Paca, J. (2007). Treatment of wet process hardboard plant VOC emissions by a pilot scale biological system, Biochemical Engineering Journal, 37, 261-270. https://doi.org/10.1016/j.bej.2007.05.005
  23. Saravanan, V. and Rajamohan, N. (2009). Treatment of xylene polluted air using press mud-based biofilter, Journal of Hazardous Materials, 162, 981-988. https://doi.org/10.1016/j.jhazmat.2008.05.158
  24. Sempere, F., Gabaldon, C., Martinez-Soria, V., Marzal, P., and Penya-roja, J. M. (2008). Performance evaluation of a biotrickling filter treating a mixture of oxygenated VOCs during intermittent loading, Chemosphere, 73, 1533-1539. https://doi.org/10.1016/j.chemosphere.2008.08.037