Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
권호 표지

Isolation and Characterization of Plant-Derived Lactic Acid Bacteria as Potential Probiotic

잠재적 생균제로서 식물 젖산균의 분리 및 특성

  • Kim, Jeong-Do (College of Natural Resources and Life Science, Pusan National University) ;
  • Park, Sung-Bo (College of Natural Resources and Life Science, Pusan National University) ;
  • Lee, Na-Ri (College of Natural Resources and Life Science, Pusan National University) ;
  • Jeong, Jin-Ha (College of Natural Resources and Life Science, Pusan National University) ;
  • Lee, Hee-Seob (Department of Food Science and Nutrition, Pusan National University) ;
  • Hwang, Dae-Youn (College of Natural Resources and Life Science, Pusan National University) ;
  • Lee, Jong-Sup (Sandong Processing Plant, Nonghyup) ;
  • Jeong, Seong-Yun (Department of Medical Life Science, Catholic University of Daegu) ;
  • Son, Hong-Joo (College of Natural Resources and Life Science, Pusan National University)
  • 김정도 (부산대학교 생명자원과학대학) ;
  • 박성보 (부산대학교 생명자원과학대학) ;
  • 이나리 (부산대학교 생명자원과학대학) ;
  • 정진하 (부산대학교 생명자원과학대학) ;
  • 이희섭 (부산대학교 식품영양학과) ;
  • 황대연 (부산대학교 생명자원과학대학) ;
  • 이종섭 (밀양 산동농협) ;
  • 정성윤 (대구가톨릭대 의생명과학과) ;
  • 손홍주 (부산대학교 생명자원과학대학)
  • Received : 2011.07.26
  • Accepted : 2011.09.04
  • Published : 2011.09.28
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Abstract

Plant lactic acid bacteria were isolated from plant-associated fermentative foods and crops, and their probiotic properties were investigated. Isolates K27 and O2 were isolated from Kimchi and onion, and identified as Lactobacillus plantarum on the basis of 16S rRNA gene analysis. The two strains were highly resistant to acid (an MRS broth at pH 2.5), where the survival rates of L. plantarum K27 and L. plantarum O2 were 90.2% and 97.3%, respectively. L. plantarum K27 and L. plantarum O2 also showed high bile resistance to 0.5% oxgall, with a more than 70% survival rate. They showed an inhibitory effect against pathogenic strains of Escherichia coli KCCM 40880 and Pseudomonas aeruginosa ATCC 10145. The antibacterial effect of the two strains was probably due to the presence of lactic acid. ACE inhibitory activities of the two strains ranged from 72.8% to 80.6% in MRS broth. Notably, the two strains showed high ACE inhibitory activity (89.2~98.2%) in MRS broth containing 10% skim milk. Antioxidant activity was tested by DPPH radical scavenging activity, with antioxidant activities of the strains being in the range of 56.8~61.5%. The results obtained in this study suggest that L. plantarum K27 and L. plantarum O2 may be potential probiotic starter cultures with applications with fermentative products.

Keywords

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