DOI QR코드

DOI QR Code

Curative Effect of Selenium Against Indomethacin-Induced Gastric Ulcers in Rats

  • Kim, Jeong-Hwan (Department of Biomaterial Control, Dong-Eui University) ;
  • Kim, Byung-Woo (Department of Biomaterial Control, Dong-Eui University) ;
  • Kwon, Hyun-Ju (Department of Biomaterial Control, Dong-Eui University) ;
  • Nam, Soo-Wan (Department of Biomaterial Control, Dong-Eui University)
  • Received : 2010.12.16
  • Accepted : 2010.12.31
  • Published : 2011.04.28

Abstract

Indomethacin is a nonsteroid anti-inflammatory agent that is known to induce severe gastric mucosal lesions. In this study, we investigated the effect of selenium on gastric mucosal lesions in rats. To confirm the curative effect of selenium against indomethacin-induced gastric ulcers, gastric ulcers were induced by oral administration of 25 mg/kg indomethacin, and then different doses (10, 50, and 100 ${\mu}g$/kg of body weight) of selenium or vehicle were treated by oral gavage for 3 days. Oral administration of indomethacin clearly increased the gastric ulcer area in the stomach, whereas selenium applied for 3 days significantly decreased the gastric ulcer area in a dose-dependent manner. In addition, selenium markedly reduced the increase of lipid peroxidation induced by indomethacin in the gastric mucosa and increased activities of radical scavenging enzymes such as superoxide dismutase, catalase, and glutathione peroxidase in a dose-dependent manner. These results reveal that selenium can heal indomethacin-induced gastric ulcers through elimination of the lipid peroxides and activation of radical scavenging enzymes.

Keywords

References

  1. Aebi, H. 1974. In H. U. Bergmeyer (ed.). Methods of Enzymatic Analysis. Academic Press, New York, pp. 674-678.
  2. Beck, W. S., H. T. Schneider, K. Dietzel, B. Nuernberg, and K. Brune. 1990. Gastrointestinal ulcerations induced by antiinflammatory drugs in rats. Arch. Toxicol. 64: 210-217. https://doi.org/10.1007/BF02010727
  3. Bjarnason, I., J. Hayllar, A. J. MacPherson, and A. Russell. 1993. Side effects of nonsteroidal anti-inflammatory drugs on the small and large intestine in humans. Gastroenterology 104: 1832-1847.
  4. Bradford, M. M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72: 248-254. https://doi.org/10.1016/0003-2697(76)90527-3
  5. Cooke, C. E. 1996. Disease management: Prevention of NSAIDinduced gastropathy. Drug Benefit Trends 8: 14-22.
  6. Del Soldato, P., D. Foschi, G. Benoni, and C. Scarpignato. 1985. Oxygen free radicals interact with indomethacin to cause gastrointestinal injury. Agents Actions 17: 484-488.
  7. Djahanguiri, B. 1969. The production of acute gastric ulceration by indomethacin in the rat. Scand. J. Gastroenterol. 4: 265-267.
  8. Ehsanullah, R. B., M. C. Page, G. Tildesley, and J. R. Wood. 1988. Prevention of gastroduodenal damage induced by nonsteroidal anti-inflammatory drugs: Controlled trial of ranitidine. Br. Med. J. 297: 1017-1021.
  9. Halliwell, B. and J. M. Gutteridge. 1992. Biologically relevant metal ion-dependent hydroxyl radical generation: An update. FEBS Lett. 307: 108-112. https://doi.org/10.1016/0014-5793(92)80911-Y
  10. Hart, F. D. and P. L. Boardman. 1963. Indomethacin: A new non-steroid anti-inflammatory agent. Br. Med. J. 2: 965-970. https://doi.org/10.1136/bmj.2.5363.965
  11. Hogeboom, G. H. 1955. In Colowick, S. P. and N. O. Kaplan (eds.). Methods in Enzymology. Academic Press, New York, pp. 16-19.
  12. Imam, S. Z., G. D. Newport, F. Islam, W. J. Slikker, and S. F. Ali. 1999. Selenium, an antioxidant, protects against methamphetamineinduced dopaminergic neurotoxicity. Brain Res. 818: 575-578. https://doi.org/10.1016/S0006-8993(98)01311-0
  13. Kim, J. H., S. K. Choi, W. J. Lim, and H. I. Chang. 2004. Protective effect of astaxanthin produced by Xanthophyllomyces dendrorhous mutant on indomethacin-induced gastric mucosal injury in rats. J. Microbiol. Biotechnol. 14: 996-1003.
  14. Lanza, F. L. 1984. Endoscopic studies of gastric and duodenal injury after the use of ibuprofen, aspirin, and other nonsteroidal anti-inflammatory agents. Am. J. Med. 13: 19-24.
  15. Lawrence, R. A. and R. F. Burk. 1976. Glutathione peroxidase activity in selenium-deficient rat liver. Biochem. Biophys. Res. Commun. 71: 952-958. https://doi.org/10.1016/0006-291X(76)90747-6
  16. Lee, S. R., S. Bar-Noy, S. Kwon, R. L. Levine, T. C. Stadtman, and S. G. Rhee. 2000. Mammalian thioredoxin reductase: Oxidation of the C-terminal cysteine/selenocysteine active site forms a thioselenide, and replacement of selenium with sulfur markedly reduces catalytic activity. Proc. Natl. Acad. Sci. USA 97: 2521- 2526. https://doi.org/10.1073/pnas.050579797
  17. Marcocci, L., L. Floche, and L. Packer. 1997. Evidence for a functional role of the selenocysteine residue in mammalian thioredoxin reductase. Biofactors 6: 351-358. https://doi.org/10.1002/biof.5520060305
  18. McCarthy, D. M. 1995. Mechanisms of mucosal injury and healing: The role of nonsteroidal anti-inflammatory drugs. Scand. J. Gastroenterol. 208: 24-29.
  19. McCord, J. M. and I. Fridovich. 1967. Superoxide dismutase, an enzymatic function for erythrocuprein (hemocuprein). J. Biol. Chem. 244: 6049-6055.
  20. Mihara, M. and M. Uchiyama. 1978. Determination of malonaldehyde precursor in tissues by thiobarbituric acid test. Anal. Biochem. 86: 271-278. https://doi.org/10.1016/0003-2697(78)90342-1
  21. Ohkawa, H., N. Ohishi, and K. Yagi. 1979. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal. Biochem. 95: 351-358. https://doi.org/10.1016/0003-2697(79)90738-3
  22. Parks, D. A. 1989. Oxygen radicals: Mediators of gastrointestinal pathophysiology. Gut 30: 293-298. https://doi.org/10.1136/gut.30.3.293
  23. Rotruck, J. T., A. L. Pope, H. E. Ganther, A. B. Swanson, D. G. Hafeman, and W. G. Hoekstra. 1973. Selenium: Biochemical role as a component of glutathione peroxidase. Science 179: 588-590. https://doi.org/10.1126/science.179.4073.588
  24. Singh, G. 1998. Recent considerations in nonsteroidal antiinflammatory drug gastropathy. Am. J. Med. 1.05(Suppl. 1B): 31-38.
  25. Takeuchi, K., K. Ueshima, Y. Hironaka, Y. Fujioka, J. Matsumoto, and S. Okabe. 1991. Oxygen free radicals and lipid peroxidation in the pathogenesis of gastric mucosal lesions induced by indomethacin in rats. Digestion 49: 175-184. https://doi.org/10.1159/000200718
  26. Tanaka, J. and Y. Yuda. 1996. Lipid peroxidation in gastric mucosal lesions induced by indomethacin in rats. Biol. Pharm. Bull. 19: 716-720. https://doi.org/10.1248/bpb.19.716
  27. Tenenbaum, J. 1999. The epidemiology of nonsteroidal antiinflammatory drugs. Can. J. Gastroenterol. 13: 119-122.
  28. Vaananen, P. M., J. B. Meddings, and J. L. Wallace. 1991. Role of oxygen-derived free radicals in indomethacin-induced gastric injury. Am. J. Physiol. 261: G470-G475.
  29. Yoshikawa, T., Y. Naito, S. Ueda, H. Oyamada, T. Takemura, N. Yoshida, S. Sugino, and M. Kondo. 1990. Role of oxygenderived free radicals in the pathogenesis of gastric mucosal lesions in rats. J. Clin. Gastroenterol. 12: 65-71.

Cited by

  1. Papel de los antioxidantes en la prevención del cáncer vol.16, pp.1, 2011, https://doi.org/10.1016/s2173-1292(12)70067-4
  2. Therapeutic potential of selenium and tellurium compounds: Opportunities yet unrealised vol.41, pp.21, 2011, https://doi.org/10.1039/c2dt12225a
  3. The suppression of thymic stromal lymphopoietin expression by selenium vol.43, pp.2, 2011, https://doi.org/10.1007/s00726-011-1156-z
  4. Effect of selenium and grape seed extract on indomethacin-induced gastric ulcers in rats vol.69, pp.3, 2011, https://doi.org/10.1007/s13105-013-0241-z
  5. Selenium: a brief review and a case report of selenium responsive cardiomyopathy vol.13, pp.1, 2013, https://doi.org/10.1186/1471-2431-13-39
  6. The Protective Effect of Aged Garlic Extract on Nonsteroidal Anti-Inflammatory Drug-Induced Gastric Inflammations in Male Albino Rats vol.2014, pp.None, 2011, https://doi.org/10.1155/2014/759642
  7. Effect of glucocorticoids on indomethacin-induced gastric ulcer in the adult male albino rat – histological, morphometric and electron microscopy study vol.10, pp.2, 2014, https://doi.org/10.5114/aoms.2012.28807
  8. Centella asiaticaLeaf Extract Protects Against Indomethacin-Induced Gastric Mucosal Injury in Rats vol.19, pp.1, 2011, https://doi.org/10.1089/jmf.2015.3464
  9. Antiulcer and hepatoprotective effects of aqueous extract of Plantago ovata seed on indomethacin-ulcerated rats vol.41, pp.1, 2011, https://doi.org/10.1016/j.bj.2018.01.001
  10. Indomethacin-induced gastric damage in rats and the protective effect of donkey milk vol.14, pp.3, 2011, https://doi.org/10.5114/aoms.2016.59645
  11. The gastroprotective effect ofN-acetylcysteine and genistein in indomethacin-induced gastric injury in rats vol.96, pp.11, 2011, https://doi.org/10.1139/cjpp-2017-0730
  12. Chemical composition and protective role of Pulicaria undulata (L.) C.A. Mey. subsp. undulata against gastric ulcer induced by ethanol in rats vol.5, pp.3, 2011, https://doi.org/10.1016/j.heliyon.2019.e01359
  13. Gastro-Protective and Anti-Oxidant Potential of Althaea officinalis and Solanum nigrum on Pyloric Ligation/Indomethacin-Induced Ulceration in Rats vol.8, pp.11, 2011, https://doi.org/10.3390/antiox8110512
  14. Selenium Nanoparticles-Embedded Chitosan Microspheres and Their Effects Upon Alcohol-Induced Gastric Mucosal Injury in Rats: Rapid Preparation, Oral Delivery, and Gastroprotective Potential of Seleniu vol.15, pp.None, 2011, https://doi.org/10.2147/ijn.s237089
  15. Effect of Cuttlebone on Healing of Indomethacin-Induced Acute Gastric Mucosal Lesions in Rats vol.2020, pp.None, 2020, https://doi.org/10.1155/2020/9592608
  16. Tetramethylpyrazine ameliorates indomethacin-induced gastric ulcer in rats: Impact on oxidative, inflammatory, and angiogenic machineries vol.28, pp.8, 2020, https://doi.org/10.1016/j.jsps.2020.06.012
  17. Anti-Inflammatory Effect of Phytoncide in an Animal Model of Gastrointestinal Inflammation vol.26, pp.7, 2021, https://doi.org/10.3390/molecules26071895
  18. Protective and therapeutic effects of the flavonoid “pinocembrin” in indomethacin-induced acute gastric ulcer in rats: impact of anti-oxidant, anti-inflammatory, and anti-apoptotic mechani vol.394, pp.7, 2011, https://doi.org/10.1007/s00210-021-02067-5
  19. Preclinical Pharmacokinetics and Acute Toxicity in Rats of 5-{[(2E)-3-Bromo-3-carboxyprop-2-enoyl]amino}-2-hydroxybenzoic Acid: A Novel 5-Aminosalicylic Acid Derivative with Potent Anti-Inflammatory A vol.26, pp.22, 2011, https://doi.org/10.3390/molecules26226801