Journal of Microbiology and Biotechnology

  • 제21권5호
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  • Pages.509-514
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  • 2011
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  • 1017-7825(pISSN)
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  • 1738-8872(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
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The Phenotypic and Genotypic Characterization of Korean Isolates of Cronobacter spp. (Enterobacter sakazakii)

  • Kim, Jung-Beom (Division of Health Research and Planning, Gyeonggi-do Research Institute of Health and Environment) ;
  • Kang, Suk-Ho (Division of Health Research and Planning, Gyeonggi-do Research Institute of Health and Environment) ;
  • Park, Yong-Bae (Division of Health Research and Planning, Gyeonggi-do Research Institute of Health and Environment) ;
  • Choi, Jae-Ho (School of Bioscience and Biotechnology, Kangwon National University) ;
  • Park, Sung-Jin (School of Bioscience and Biotechnology, Kangwon National University) ;
  • Cho, Seung-Hak (Division of Enteric Bacterial Infections, Center for Infectious Diseases, National Institute of Health) ;
  • Park, Mi-Sun (Division of Enteric Bacterial Infections, Center for Infectious Diseases, National Institute of Health) ;
  • Lee, Hae-Kyung (Department of Laboratory Medicine, Catholic University College of Medicine) ;
  • Choi, Na-Jung (School of Bioscience and Biotechnology, Kangwon National University) ;
  • Kim, Ha-Na (School of Bioscience and Biotechnology, Kangwon National University) ;
  • Oh, Deog-Hwan (School of Bioscience and Biotechnology, Kangwon National University)
  • 투고 : 2010.07.30
  • 심사 : 2011.02.11
  • 발행 : 2011.05.28
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초록

This study was conducted to investigate the phenotypic and genotypic characteristics of Korean isolates of Cronobacter spp. (Enterobacter sakazakii). A total of 43 Cronobacter spp., including 5 clinical isolates, 34 food isolates, 2 environmental isolates, and 2 reference strains (C. sakazakii ATCC 29004 and C. muytjensii ATCC51329) were used in this study. Korean isolates of Cronobacter spp. were divided into 11 biogroups according to their biochemical profiles and 3 genomic groups based on the analysis of their 16S rRNA gene sequences. Biogroups 1 and 2 contained the majority of isolates (n=26), most of which were contained in 16S rRNA cluster 1 (n=34). Korean isolates of Cronobacter spp. showed diverse biochemical profiles. Biogroup 1 contained C. sakazakii GIHE (Gyeonggido Research Institute of Health and Environment) 1 and 2, which were isolated from babies that exhibited symptoms of Cronobacter spp. infection such as gastroenteritis, sepsis, and meningitis. Our finding revealed that Biogroup 1, C. sakazakii, is more prevalent and may be a more pathogenic biogroup than other biogroups, but the pathogenic biogroup was not represented clearly among the 11 biogroups tested in this study. Thus, all biogroups of Cronobacter spp. were recognized as pathogenic bacteria, and the absence of Cronobacter spp. in infant foods should be constantly regulated to prevent food poisoning and infection caused by Cronobacter spp.

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참고문헌

  1. Barker, D. 2004. LVB: Parsimony and simulated annealing in the search for phylogenetic trees. Bioinformatics 20: 274-275. https://doi.org/10.1093/bioinformatics/btg402
  2. Biering, G., S. Karlsson, N. C. Clark, K. E. Jonsdottir, and P. Ludvigsson. 1989. Three cases of neonatal meningitis caused by Enterobacter sakazakii in powdered milk. J. Clin. Microbiol. 27: 2054-2056.
  3. Breeuwer, P., A. Lardeau, M. Peterz, and H. M. Joosten. 2003. Desiccation and heat tolerance of Enterobacter sakazakii. J. Appl. Microbiol. 95: 967-973.
  4. Drudy, D., M. O'Rourke, M. Murphy, N. R. Mullane, R. O'Mahony, L. Kelly, et al. 2006. Characterization of a collection of Enterobacter sakazakii isolates from environment and food sources. Int. J. Food Microbiol. 110: 127-134. https://doi.org/10.1016/j.ijfoodmicro.2006.02.008
  5. Farmer III, J. J., M. A. Asbury, F. W. Hickman, Don J. Brenner, and The Enterobacteriaceae Study Group. 1980. Enterobacter sakazakii: A new species of "Enterobacteriaceae" isolated from clinical specimens. Int. J. Syst. Bacteriol. 30: 569-584. https://doi.org/10.1099/00207713-30-3-569
  6. Iversen, C. and S. Forsythe. 2003. Risk profile of Enterobacter sakazakii, an emergent pathogen associated with infant milk formula. Trends Food Sci. Technol. 14: 443-454. https://doi.org/10.1016/S0924-2244(03)00155-9
  7. Iversen, C., M. Waddington, S. L. On, and S. Forsythe. 2004. Identification and phylogeny of Enterobacter sakazakii relative to Enterobacter and Citrobacter species. J. Clin. Microbiol. 42: 5368-5370. https://doi.org/10.1128/JCM.42.11.5368-5370.2004
  8. Iversen, C., P. Druggan, and S. Forsythe. 2004. A selective differential medium for Enterobacter sakazakii, a preliminary study. Int. J. Food Microbiol.96: 133-139. https://doi.org/10.1016/j.ijfoodmicro.2004.01.024
  9. Iversen, C., M. Waddington, J. J. Farmer III, and S. J. Forsythe. 2006. The biochemical differentiation of Enterobacter sakazakii genotypes. BMC Microbiol. 6: 94-100. https://doi.org/10.1186/1471-2180-6-94
  10. Iversen, C., A. Lehner, N. Mullane, E. Bidlas, I. Cleenwerck, J. Marugg, S. Fanning, R. Stephan, and H. Joosten. 2007. The taxonomy of Enterobacter sakazakii: Proposal of a new genus Cronobacter gen. nov. and descriptions of Cronobacter sakazakii comb. nov. Cronobacter sakazakii subsp. sakazakii, comb. nov., Cronobacter sakazakii subsp. malonaticus subsp. nov., Cronobacter turicensis sp. nov., Cronobacter muytjensii sp. nov., Cronobacter dublinensis sp. nov. and Cronobacter 1. BMC Evol. Biol. 7: 64-74. https://doi.org/10.1186/1471-2148-7-64
  11. Iversen, C., N. Mullane, B. McCardell, B. D. Tall, E. Bidlas, A. Lehner, S. Fanning, R. Stephan, and H. Joosten. 2008. Cronobacter gen. nov., a new genus to accommodate the biogroups of Enterobacter sakazakii, and proposal of Cronobacter sakazakii gen. nov., comb. nov., Cronobacter malonaticus sp. nov., Cronobacter turicensis sp. nov., Cronobacter muytjensii sp. nov., Cronobacter dublinensis sp. nov., Cronobacter genomospecies 1, and of three subspecies, Cronobacter dublinensis subsp. dublinensis subsp. nov., Cronobacter dublinensis subsp. lausannensis subsp. nov. and Cronobacter dublinensis subsp. lactaridi subsp. nov. Int. J. Syst. Evol. Microbiol 58: 1442-1447. https://doi.org/10.1099/ijs.0.65577-0
  12. Jung, M. K. and J. H. Park. 2006. Prevalence and thermal stability of Enterobacter sakazakii from unprocessed ready-toeat agricultural products and powdered infant formulas. Food Sci. Biotechnol. 15: 152-157.
  13. Kim, J. B., S. H. Cho, Y. B. Park, J. B. Lee, J. C. Kim, B. K. Lee, H. K. Lee, and H. S. Chae. 2008. Surveillance of stool samples for the presence of Enterobacter sakazakii among Korean people. Yonsei Med. J. 49: 1017-1022. https://doi.org/10.3349/ymj.2008.49.6.1017
  14. Kolbert, C. P. and D. H. Persing. 1999. Ribosomal DNA sequencing as a tool for identification of bacterial pathogens. Curr. Opin. Microbiol. 2: 299-305. https://doi.org/10.1016/S1369-5274(99)80052-6
  15. Korea Food and Drug Administration. 2008. KFDA. Korea Food Code. P. 10-3-43. Seoul, Korea.
  16. Leclercq, A., C. Wanegue, and P. Baylac. 2002. Comparison of fecal coliform agar and violet red bile lactose agar for fecal coliform enumeration in foods. Appl. Environ. Microbiol. 68: 1631-1638. https://doi.org/10.1128/AEM.68.4.1631-1638.2002
  17. Lehner, A. and R. Stephan. 2004. Microbiological, epidemical, and food safety aspects of Enterobacter sakazakii. J. Food Prot. 67: 2850-2857.
  18. Nazarowec-White, M. and J. M. Farber. 1997. Enterobacter sakazakii: a Review. Int. J. Food Microbiol. 34: 103-113. https://doi.org/10.1016/S0168-1605(96)01172-5
  19. Sanger, F., S. Nicklen, and A. R. Coulson. 1992. DNA sequencing with chain-terminating inhibitors. 1977. Biotechnology 24: 104- 108.
  20. Simmons, B. P., M. S. Gelfand, M. Haas, L. Metts, and J. Ferguson. 1989. Enterobacter sakazakii infections in neonates associated with intrinsic contamination of a powdered infant formula. Infect. Cont. Hosp. Epidemiol. 10: 398-401. https://doi.org/10.1086/646060
  21. US Food and Drug Administration. 2002. Isolation and enumeration of Enterobacter sakazakii from dehydrated powdered infant formula. Available at http://www.cfsan.fda.gov/-comm/mmesakaz. html. Accessed 10 December 2009.
  22. Van Acker, J., F. de Smet, G. Muyldermans, A. Bougatef, A. Naessens, and S. Lauwers. 2001. Outbreak of necrotizing enterocolitis associated with Enterobacter sakazakii in powdered milk formula. J. Clin. Microbiol. 39: 293-297. https://doi.org/10.1128/JCM.39.1.293-297.2001
  23. Yoo, M. K., S. S. Kim, and S. S. Oh. 2005. Isolation and genotyping of Enterobacter sakazakii from powdered infant formula manufactured in Korea. Food Sci. Biotechnol. 14: 875- 877.

피인용 문헌

  1. The Genotypic Characterization of Cronobacter spp. Isolated in China vol.9, pp.7, 2011, https://doi.org/10.1371/journal.pone.0102179
  2. Occurrence and Characterization of Cronobacter spp. in Dehydrated Rice Powder from Chinese Supermarket vol.10, pp.7, 2011, https://doi.org/10.1371/journal.pone.0131053
  3. Surveillance and molecular typing of Cronobacter spp. in commercial powdered infant formula and follow‐up formula from 2011 to 2013 in Shandong Province, China vol.97, pp.7, 2011, https://doi.org/10.1002/jsfa.8021