Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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The Phenotypic and Genotypic Characterization of Korean Isolates of Cronobacter spp. (Enterobacter sakazakii)

  • Kim, Jung-Beom (Division of Health Research and Planning, Gyeonggi-do Research Institute of Health and Environment) ;
  • Kang, Suk-Ho (Division of Health Research and Planning, Gyeonggi-do Research Institute of Health and Environment) ;
  • Park, Yong-Bae (Division of Health Research and Planning, Gyeonggi-do Research Institute of Health and Environment) ;
  • Choi, Jae-Ho (School of Bioscience and Biotechnology, Kangwon National University) ;
  • Park, Sung-Jin (School of Bioscience and Biotechnology, Kangwon National University) ;
  • Cho, Seung-Hak (Division of Enteric Bacterial Infections, Center for Infectious Diseases, National Institute of Health) ;
  • Park, Mi-Sun (Division of Enteric Bacterial Infections, Center for Infectious Diseases, National Institute of Health) ;
  • Lee, Hae-Kyung (Department of Laboratory Medicine, Catholic University College of Medicine) ;
  • Choi, Na-Jung (School of Bioscience and Biotechnology, Kangwon National University) ;
  • Kim, Ha-Na (School of Bioscience and Biotechnology, Kangwon National University) ;
  • Oh, Deog-Hwan (School of Bioscience and Biotechnology, Kangwon National University)
  • Received : 2010.07.30
  • Accepted : 2011.02.11
  • Published : 2011.05.28
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Abstract

This study was conducted to investigate the phenotypic and genotypic characteristics of Korean isolates of Cronobacter spp. (Enterobacter sakazakii). A total of 43 Cronobacter spp., including 5 clinical isolates, 34 food isolates, 2 environmental isolates, and 2 reference strains (C. sakazakii ATCC 29004 and C. muytjensii ATCC51329) were used in this study. Korean isolates of Cronobacter spp. were divided into 11 biogroups according to their biochemical profiles and 3 genomic groups based on the analysis of their 16S rRNA gene sequences. Biogroups 1 and 2 contained the majority of isolates (n=26), most of which were contained in 16S rRNA cluster 1 (n=34). Korean isolates of Cronobacter spp. showed diverse biochemical profiles. Biogroup 1 contained C. sakazakii GIHE (Gyeonggido Research Institute of Health and Environment) 1 and 2, which were isolated from babies that exhibited symptoms of Cronobacter spp. infection such as gastroenteritis, sepsis, and meningitis. Our finding revealed that Biogroup 1, C. sakazakii, is more prevalent and may be a more pathogenic biogroup than other biogroups, but the pathogenic biogroup was not represented clearly among the 11 biogroups tested in this study. Thus, all biogroups of Cronobacter spp. were recognized as pathogenic bacteria, and the absence of Cronobacter spp. in infant foods should be constantly regulated to prevent food poisoning and infection caused by Cronobacter spp.

Keywords

References

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