Journal of Microbiology and Biotechnology

  • 제21권10호
  • /
  • Pages.1073-1080
  • /
  • 2011
  • /
  • 1017-7825(pISSN)
  • /
  • 1738-8872(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
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Effects of Carbon and Nitrogen Sources on Fatty Acid Contents and Composition in the Green Microalga, Chlorella sp. 227

  • Cho, Sun-Ja (Department of Civil and Environmental Engineering, Pusan National University) ;
  • Lee, Duk-Haeng (Department of Civil and Environmental Engineering, Pusan National University) ;
  • Luong, Thao Thanh (Department of Civil and Environmental Engineering, Pusan National University) ;
  • Park, So-Ra (Department of Civil and Environmental Engineering, Pusan National University) ;
  • Oh, You-Kwan (Bioenergy Research Center, Korea Institute of Energy Research) ;
  • Lee, Tae-Ho (Department of Civil and Environmental Engineering, Pusan National University)
  • 투고 : 2011.03.28
  • 심사 : 2011.07.08
  • 발행 : 2011.10.28
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초록

In order to investigate and generalize the effects of carbon and nitrogen sources on the growth of and lipid production in Chlorella sp. 227, several nutritional combinations consisting of different carbon and nitrogen sources and concentrations were given to the media for cultivation of Chlorella sp. 227, respectively. The growth rate and lipid content were affected largely by concentration rather than by sources. The maximum specific growth was negatively affected by low concentrations of carbon and nitrogen. There is a maximum allowable inorganic carbon concentration (less than 500~1,000 mM bicarbonate) in autotrophic culture, but the maximum lipid content per gram dry cell weight (g DCW) was little affected by the concentration of inorganic carbon within the concentration. The lipid content per g DCW was increased when the microalga was cultured with the addition of glucose and bicarbonate (mixotrophic) at a fixed nitrogen concentration and with the lowest nitrogen concentration (0.2 mM), relatively. Considering that lipid contents per g DCW increased in those conditions, it suggests that a high ratio of carbon to nitrogen in culture media promotes lipid accumulation in the cells. Interestingly, a significant increase of the oleic acid amount to total fatty acids was observed in those conditions. These results showed the possibility to induce lipid production of high quality and content per g DCW by modifying the cultivation conditions.

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