Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Isolation, Purification, and Enzymatic Characterization of Extracellular Chitosanase from Marine Bacterium Bacillus subtilis CH2

  • Oh, Chul-Hong (Korea Ocean Research and Development Institute) ;
  • Zoysa, Mahanama De (College of Veterinary Medicine, Chungnam National University) ;
  • Kang, Do-Hyung (Korea Ocean Research and Development Institute) ;
  • Lee, Young-Deuk (Department of Marine Life Sciences, School of Marine Biomedical Sciences, Jeju National University) ;
  • Whang, Il-Son (Department of Marine Life Sciences, School of Marine Biomedical Sciences, Jeju National University) ;
  • Nikapitiya, Chamilani (Department of Fisheries, Animal and Veterinary Science, University of Rhode Island) ;
  • Heo, Soo-Jin (Korea Ocean Research and Development Institute) ;
  • Yoon, Kon-Tak (Korea Ocean Research and Development Institute) ;
  • Affan, Abu (Korea Ocean Research and Development Institute) ;
  • Lee, Je-Hee (Department of Marine Life Sciences, School of Marine Biomedical Sciences, Jeju National University)
  • Received : 2011.04.20
  • Accepted : 2011.06.15
  • Published : 2011.10.28
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Abstract

A Bacillus subtilis strain was isolated from the intestine of Sebastiscus marmoratus (scorpion fish) that was identified as Bacillus subtilis CH2 by morphological, biochemical, and genetic analyses. The chitosanase of Bacillus subtilis CH2 was best induced by fructose and not induced with chitosan, unlike other chitosanases. The strain was incubated in LB broth, and the chitosanase secreted into the medium was concentrated with ammonium sulfate precipitation and purified by gel permeation chromatography. The molecular mass of the purified chitosanase was detected as 29 kDa. The optimum pH and temperature of the purified chitosanase were 5.5 and $60^{\circ}C$, respectively. The purified chitosanase was continuously thermostable at $40^{\circ}C$. The specific acitivity of the purified chitosanase was 161 units/mg. The N-terminal amino acid sequence was analyzed for future study.

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References

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