Reproductive and Developmental Biology

The Korean Society of Animal Reproduction (한국동물번식학회)
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The Change of Protein Patterns in Follicular Fluid on Ovarian Response Following Controlled Ovarian Hyperstimulation (COH) of Human

사람의 과배란 유도 후 난소 반응별 난포액 내 단백질 변화

  • 이채식 (세화병원 불임연구소) ;
  • 이상찬 (세화병원 불임연구소) ;
  • 노용호 (세화병원 불임연구소) ;
  • 오대식 (세화병원 불임연구소) ;
  • 이용승 (강원대학교 동물생명과학대학) ;
  • 송은지 (강원대학교 동물생명과학대학) ;
  • 정희태 (강원대학교 수의과대학) ;
  • 양부근 (강원대학교 동물생명과학대학) ;
  • 박춘근 (강원대학교 동물생명과학대학)
  • Received : 2011.08.17
  • Accepted : 2011.08.24
  • Published : 2011.09.30
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Abstract

It was conducted the experiment, divided into three groups as normal, poor and polycystic ovary syndrome, to detect the change of protein patterns in follicular fluid on ovarian response following controlled ovarian hyperstimulation for human IVF outcome. In the normal group, it was confirmed reproducible 57 spots in the detected total 81 spots. Then 1 spot was not found in the other groups. In the poor responder group, it was found reproducible 53 spots in the detected total 98 spots. 6 spots were down-regulation and 7 spots were up-regulation comparable with normal group. There were not 5 spots in poor responder group comparable with other groups. In the polycystic ovary syndrome group, it was expressed reproducible 53 spots in the detected total 80 spots and 3 spots were just expressed in this group. However, 4 spots were not found in polycystic ovary syndrome. 9 spots were up-regulation comparable with normal group. Significant up and down-regulation spots among the each groups were identified. The results were a cytosolic carboxypeptidase, a signal-induced proliferation-associated protein 1, a ceruloplasmin, a keratin(type II cytoskeletal 1), a polypeptide N-acetylgalactosantinyltransferase 2, a serine/threonine-protein phosphatase 4 regulatory subunit 4. It was identified that 8 spots, 6 kinds of protein are corresponded with NCBInr database research, but 10 spots were failed in the identification. In conclusion, it has been confirmed change and expression of protein on the ovarian response following COH of human.

Keywords

References

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