Reproductive and Developmental Biology

  • 제35권3호
  • /
  • Pages.239-245
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  • 2011
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  • 1738-2432(pISSN)
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  • 2288-0151(eISSN)
한국동물번식학회 (The Korean Society of Animal Reproduction)
권호 표지

Phosphoprotein Enriched in Astrocytes 15 (PEA15)가 과발현하는 형질전환 돼지의 생산

Production of a Transgenic Enriched in Pig Overexpressing Phosphoprotein Astrocytes 15 (PEA 15)

  • 이휘철 (농촌진흥청 국립축산과학원) ;
  • 김현미 (농촌진흥청 국립축산과학원) ;
  • 이승훈 (농촌진흥청 국립축산과학원) ;
  • 오건봉 (농촌진흥청 국립축산과학원) ;
  • 정학재 (농촌진흥청 국립축산과학원) ;
  • 양병철 (농촌진흥청 국립축산과학원) ;
  • 김경운 (농촌진흥청 국립축산과학원) ;
  • 이풍연 (농촌진흥청 국립축산과학원) ;
  • 박진기 (농촌진흥청 국립축산과학원) ;
  • 장원경 (농촌진흥청 국립축산과학원)
  • 투고 : 2011.09.02
  • 심사 : 2011.09.09
  • 발행 : 2011.09.30
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초록

The overexpression of Phosphoprotein Enriched in Astrocytes (PEA15) gene is commonly found in human diabetic patients. The overexpression of this gene in skeletal muscle and fat tissues have been reported to cause insulin resistance, thereby impairing insulin stimulated glucose uptake. We introduced a gene of mouse PEA15 (mPEA15) and enhanced green fluorescent protein (EGFP) into fertilized one cell pig zygotes using microinjection, and produced a piglet that showed overexpression of mPEA15 in the muscle tissues and expression of EGFP in the ear tissues and hooves. RT-PCR RFLP, southern blot and FISH analysis showed that the tissues carried the transgene. Real-time RT-PCR and western blots demonstrated that PEA15 gene was overexpressed in the various tissues and muscle tissues, respectively. These fads suggest that expression vector system is normally expressed in the transgenic (TG) pigs. To use as animal diseases model for type 2 diabetes, further study is necessary to confirm whether diabetes occur in these TG pigs, especially insulin resistance.

키워드

참고문헌

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