Food Science and Preservation (한국식품저장유통학회지)

The Korean Society of Food Preservation (한국식품저장유통학회)
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Optimization of Extraction Conditions for Ethanol Extracts from Citrus unshiu Peel by Response Surface Methodology

반응표면분석에 의한 진피 에탄올 추출물의 추출조건 최적화

  • Received : 2011.05.06
  • Accepted : 2011.09.30
  • Published : 2011.10.30
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Abstract

This study was conducted to monitor the quality characteristics Citrus unshiu ethanolic extracts by a response surface methodology. In extraction conditions based on the central composite design with variations in ethanol concentration (0~100%), extraction temperature($35\sim95^{\circ}C$), and ratio of solvent to sample(20 mL/g). The maximum value of yield was 36.31% at $93.18^{\circ}C$, 67.13% of ethanol concentration. The extraction yield and total polyphenol content were improved with the increase of ethanol concentration than extraction temperature. Total flavonoid content was improved with the increase of extraction temperature than ethanol concentration. The coefficients of determinations($R^2$) were 0.8646(p<0.1) and 0.9153(p<0.05) in electron donating ability and hesperidin content, respectively. Estimated conditions for the maximized extraction including yield, total polyphenol content, total flavonoid content, electron donating ability, and hesperidin content were 55~73% in ethanol concentration, $89\sim95^{\circ}C$ in extraction temperature, and 20 mL/g in ratio of solvent to sample.

본 실험은 반응표면분석법을 이용하여 진피 에탄올 추출물의 이화학적 특성을 모니터링 하였다. 진피의 에탄올 추출조건의 최적화를 위하여 예비실험 결과에 따라 요인변수들 중 시료대 용매비를 20 mL/g로 고정하여 반응표면을 나타내었다. 추출조건에 따른 수율 및 총 폴리페놀함량의 최대값은 예측된 정상점에서 능선분석을 실시하여 본 결과, 36.31%와 13.86 mg/mL이었다. 추출조건별 총 플라보노이드함량은 3.19~6.67 mg/mL의 범위였으며, 결과에 대한 반응표면 회귀식의 R2는 0.8986로 5%이내의 유의수준에서 인정되었는데 에탄올농도보다 추출온도에 더 큰 영향을 받는 것으로 나타났다. 전자공여능에 대한 회귀식의 R2는 0.8646로 10%이내의 유의수준에서 유의성이 인정되었으며, 추출조건에 따른 hesperidin함량은 0.4282~3.823 mg/mL의 범위였으며, 추출온도보다는 에탄올농도에 많은 영향을 받음을 알 수 있었다. 진피 추출물의 특성인 수율, 총 폴리페놀함량, 총 플라보노이드함량, 전자공여능, hesperidin함량에 대해 contour map을 superimposing하여 얻은 최적 추출조건의 범위는 시료대 용매비 20 mL/g, 에탄올 농도 55~73%, 추출온도 $89\sim95^{\circ}C$로 예측되었다.

Keywords

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