Journal of the Korean Association of Oral and Maxillofacial Surgeons

  • 제37권5호
  • /
  • Pages.415-420
  • /
  • 2011
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  • 2234-7550(pISSN)
  • /
  • 2234-5930(eISSN)
대한구강악안면외과학회 (The Korean Association of Oral and Maxillofacial Surgeons)
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치아모세포와 키토산으로 강화된 생체 적합 칼슘인산시멘트와의 상호작용

Interaction between odontoblast and bio-calcium phosphate cement reinforced with chitosan

  • 전병도 (부산대학교 치의학전문대학원 구강악안면외과) ;
  • 김성원 (부산대학교 치의학전문대학원 구강악안면외과) ;
  • 이성탁 (부산대학교 치의학전문대학원 구강악안면외과) ;
  • 김태훈 (부산대학교 치의학전문대학원 구강악안면외과) ;
  • ;
  • 김규천 ;
  • 김용덕 (부산대학교 치의학전문대학원 구강악안면외과) ;
  • 김욱규 (부산대학교 치의학전문대학원 구강악안면외과)
  • Chun, Byung-Do (Department of Oral and Maxillofacial Surgery, School of Dentistry, Pusan National University) ;
  • Kim, Sung-Won (Department of Oral and Maxillofacial Surgery, School of Dentistry, Pusan National University) ;
  • Lee, Sung-Tak (Department of Oral and Maxillofacial Surgery, School of Dentistry, Pusan National University) ;
  • Kim, Tae-Hoon (Department of Oral and Maxillofacial Surgery, School of Dentistry, Pusan National University) ;
  • Lee, Jung-Han (Department of Oral and Maxillofacial Surgery, School of Dentistry, Pusan National University) ;
  • Kim, Gyoo-Cheon (Department of Oral Anatomy, School of Dentistry, Pusan National University) ;
  • Kim, Yong-Deok (Department of Oral and Maxillofacial Surgery, School of Dentistry, Pusan National University) ;
  • Kim, Uk-Kyu (Department of Oral and Maxillofacial Surgery, School of Dentistry, Pusan National University)
  • 투고 : 2011.03.03
  • 심사 : 2011.09.29
  • 발행 : 2011.10.31
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초록

Purpose: Calcium phosphate cement (CPC) is one of many useful materials for restoring tooth defects, periodontium and maxillofacial area. Chitosan is a biodegradable material that has been shown to promote the growth and differentiation of osteoblasts in culture. This study examined the interaction between odontoblasts and bio-calcium phosphate cement reinforced with chitosan. Materials and Methods: $5{\times}10^3$ odontoblastic cells were seeded into each well. Various concentrations of bio-calcium phosphate cement reinforced with chitosan (10, 20, 50, 100, 200, 500 ${\mu}g$/ml, 1, 2, 4 mg/ml) were diluted and added to the wells. The well was incubated for 24 h, 48 h and 72 h. After incubation, the number of cells was assessed to determine the cell viability. A cytokinesis-block micronucleus assay and chromosomal aberration test were carried out to estimate the extent of chromosomal abnormalities. Microscopic photographs and RT-PCR were performed to examine the adhesion potential of bio-calcium phosphate cement reinforced with chitosan. Results: Bio-CPC-reinforced chitosan did not show significant cytotoxicity. The number of damaged chromosomes in the cells treated with Bio-CPC-reinforced chitosan was similar to that in the control cells. There was no significant increase in the number of chromosomal aberrations in the Bio-CPC reinforced chitosan exposed cells. Microscopic photographs and RT-PCR confirmed the adhesive potential of bio-CPC reinforced chitosan to odontoblasts. Conclusion: Bio-CPC-reinforced chitosan did not affect the odontoblastic cell viability, and had no significant cytotoxic effect. Bio-CPC-reinforced chitosan showed adhesive potential to odontoblasts. These results are expected form the basis of future studies on the effectiveness of dental restorative materials in Bio-CPC reinforced with chitosan.

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참고문헌

  1. Pereira JC, Segala AD, Costa CAS. Human pulpal response to direct pulp capping with an adhesive system. Am J Dent 2000; 13:139-47.
  2. Cox CF, Hafez AA, Akimoto N, et al. Biocompatibility of primer, adhesive and resin composite systems on non-exposed and exposed pulps of non-human primate teeth. Am J Dent 1998; 11:S55-63.
  3. Machida Y, Nagai T, Abe M, Sannan T. Use of chitosan and hydroxypropylchitosan in drug formulations to effect sustained release. Drug Dis Deliv 1986;1:119-30.
  4. Muzzarelli RAA, Biagini G, Bellardini M, Simonelli L, Castaldini C, Fratto G. Osteoconduction exerted by methylpyrrolidinone chitosan used in dental surgery. Biomaterials 1993; 14(1):39-43 https://doi.org/10.1016/0142-9612(93)90073-B
  5. Muzzarelli RAA. Amphoteric derivatives of chitosan and their biological significance. In: Skjak-Brak G, Anthonsen T, Sandford P, editors. Chitin and chitosan. New York: Elsevier; 1989. p. 87-99.
  6. Yamaguchi I, Tokuchi K, Fukuzaki H, Koyama Y, Takakuda K, Monma H, Tanaka J. Precipitation and microstructure analysis of chitosan/hydroxyapatite nanocomposites. J Biomed Mater Res 2001;55:20-7. https://doi.org/10.1002/1097-4636(200104)55:1<20::AID-JBM30>3.0.CO;2-F
  7. LeGeros RZ. Biodegradation and bioresorption of calcium phosphate ceramics. Clin Mater 1993;14:65-88. https://doi.org/10.1016/0267-6605(93)90049-D
  8. Chang MC, Ko CC, Douglas WH. Preparation of hydroxyapatitegelatin nanocomposites. Biomaterials 2003;24:2853-62. https://doi.org/10.1016/S0142-9612(03)00115-7
  9. Brown WE, Chow LC. A new calcium phosphate water setting cement. In: Brown PW, editor. Cements research progress. Westerville, OH: Am. Ceram. Soc.; 1986. p. 352-79.
  10. Shindo ML, Contantino PD, Friedman CD, Chow LC. Facial skeletal augmentation using hydroxyapatite cement. Arch Otolaryngol Head Neck Surg 1993;119:185-90 https://doi.org/10.1001/archotol.1993.01880140069012
  11. Friedman CD, Costantino PD, Takagi S, Chow LC. BoneSourceTM hydroxyapatite cement: a novel biomaterial for craniofacial skeletal tissue engineering and reconstruction. J Biomed Mater Res 1998;43B:428-32
  12. Sugawara A, Kusama K, Nishimura S, Nishiyama M, Moro I, Kudo I, et al. Histopathological reaction of a calcium phosphate cement root canal filler. J Hard Tissue Biol 1995;4:1-7.
  13. Cherng AM, Chow LC, Takagi S. In vitro evaluation of a calcium phosphate cement root canal filler/sealer. J Endodontics 2001;27:613-5. https://doi.org/10.1097/00004770-200110000-00003
  14. Dickens-Venz SH, Takagi S, Chow LC, Bowen RL, Johnston AD, Dickens B. Physical and chemical properties of resin-reinforced calcium phosphate cements. Dent Mater 1994;10:100-6. https://doi.org/10.1016/0109-5641(94)90048-5
  15. Dickens SH, Flaim GM, Takagi S. Mechanical properties and biochemical activity of remineralizing resin-based a-PO4 cements. Dent Mater 2003;19:558-66. https://doi.org/10.1016/S0109-5641(02)00105-7
  16. Takagi S, Chow LC, Hirayama S, Eichmiller FC. Properties of novel resorbable chitosan-calcium phosphate composites. Dent Mater 2003;19:797-804. https://doi.org/10.1016/S0109-5641(03)00028-9
  17. Xu HHK, Quinn JB, Takagi S, Chow LC. Synergistic reinforcement of in situ hardening calcium phosphate composite scaffold for bone tissue engineering. Biomaterials 2004;25:1029-37. https://doi.org/10.1016/S0142-9612(03)00608-2
  18. Xu HHK, Simon Jr CG. Fast setting calcium phosphate-chitosan scaffold: mechanical properties and biocompatibility. Biomaterials 2005;26:1337-48. https://doi.org/10.1016/j.biomaterials.2004.04.043
  19. Di Martino A, Sittinger M, Risbud MV. Chitosan: a versatile biopolymer for orthopaedic tissue-engineering. Biomaterials 2005;26:5983-90. https://doi.org/10.1016/j.biomaterials.2005.03.016
  20. Prabaharan M. Review paper: chitosan derivatives as promising materials for controlled drug delivery. J Biomater Appl 2008;23: 5-36. https://doi.org/10.1177/0885328208091562
  21. Kim IY, Seo SJ, Moon HS, Yoo MK, Park IY, Kim BC, et al. Chitosan and its derivatives for tissue engineering applications. Biotechnol Adv 2008;26:1-21. https://doi.org/10.1016/j.biotechadv.2007.07.009
  22. Li X, Feng Q, Liu X, DongW, Cui F. Collagen-based implants reinforced by chitin fibres in a goat shank bone defect model. Biomaterials 2006;27:1917-23. https://doi.org/10.1016/j.biomaterials.2005.11.013
  23. Padois K, Rodriguez F. Effects of chitosan addition to self-setting bone cement. Biomed Mater Eng 2007;17:309-20.
  24. Khashaba RM, Chutkan NB, Borke JL. Comparative study of biocompatibility of newly developed calcium phosphate-based root canal sealers on fibroblasts derived from primary human gingiva and a mouse L929 cell line. Int Endod J 2009;42:711-8. https://doi.org/10.1111/j.1365-2591.2009.01572.x
  25. Dagang G, Kewei X, Haoliang S, et al. Physicochemical properties of TTCP/DCPA system cement formed in physiological saline solution and its cytotoxicity. J Biomed Mater Res A 2006; 77:313-23.
  26. Matsuya S, Takagi S, Chow LC. Effect of mixing ratio and pH on the reaction between $Ca_{4}(PO_{4})_{2}O$ and $CaHPO_{4}$. J Mater Sci Mater Med 2000;11:305-11. https://doi.org/10.1023/A:1008961314500
  27. Sun-Kyung Lee, Sang-Kwang Lee, Sang-Im Lee, Jeong-Hui Park, Jun-Hyeog Jang, Hae-Won Kim, Eun-Cheol Kim. Effect of Calcium Phosphate Cements on Growth and Odontoblastic Differentiation in Human Dental Pulp Cell. Journal of Endodontics, 2010;36(9):1537-42. https://doi.org/10.1016/j.joen.2010.04.027

피인용 문헌

  1. Injectable β -TCP/MCPM cement associated with mesoporous silica for bone regeneration: characterization and toxicity evaluation vol.13, pp.2, 2018, https://doi.org/10.1088/1748-605x/aa9085
  2. Complications after craniofacial reconstruction with calcium phosphate cements: a case report and review of the literature vol.44, pp.5, 2011, https://doi.org/10.5125/jkaoms.2018.44.5.207