Animal cells and systems

  • 제15권4호
  • /
  • Pages.310-314
  • /
  • 2011
  • /
  • 1976-8354(pISSN)
  • /
  • 2151-2485(eISSN)
한국통합생물학회 (The Korean Society for Integrative Biology)
권호 표지
DOI QR코드

DOI QR Code

Growth hormone and receptor gene mutations in Chinese Banna miniature pig

  • Deng, J.Z. (College of Animal Science and Veterinary Medicine, Jilin University) ;
  • Hao, L.L. (College of Animal Science and Veterinary Medicine, Jilin University) ;
  • Li, M.T. (College of Resources and Environment, Jilin Agricultural University) ;
  • Lang, S. (College of Animal Science and Veterinary Medicine, Jilin University) ;
  • Zeng, Y.Z. (College of Animal Science and Technology, Yunnan Agricultural University) ;
  • Liu, S.C. (College of Animal Science and Veterinary Medicine, Jilin University) ;
  • Zhang, Y.L. (College of Animal Science, Southern China Agricultural University)
  • 투고 : 2011.01.09
  • 심사 : 2011.02.15
  • 발행 : 2011.12.31
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

The Banna miniature pig (BNMP) is a representative miniature pig breed in China. Even though BNMP dwarfism is obvious, its underlying causative mutations remain unknown. In this study, the BNMP and Large White pig (LWP) serum growth hormone (GH) and insulin-like growth factor (IGF-1) levels were detected by ELISA and compared. BNMP serum IGF-1 levels were significantly lower than LWP levels (P<0.05). The miniature condition may arise from mutations in the GH and GH receptor (GHR) genes. Therefore, GH and GHR cDNA from the BNMP were cloned into a pMD18-T vector by RT-PCR using the total RNA obtained from the BNMP's pituitary and liver tissues. Sequencing results indicated that the open reading frame of the BNMP GH gene is composed of a 26-residue signal peptide and a 191-residue mature peptide. The coding sequence of the BNMP GHR gene contained 639 amino acids, including a signal peptide that is 18 amino acids long. Two amino acid substitutions, A09V and R22Q, were found in the signal peptide of the GH gene. Additionally, the S104P mutation was found in the BNMP's mature GH protein. Four mutations in the cytoplasmic domain of GHR may influence the downstream signal transduction of GHR, which needs further experimental evidence.

키워드

참고문헌

  1. Amselem S, Duquesnoy P, Attree O, Novelli G, Bousnina S, Postel-Vinay MC, Goossens M. 1989. Laron dwarfism and mutations of the growth hormone receptor gene. N Engl J Med. 321(15):989-995. https://doi.org/10.1056/NEJM198910123211501
  2. Burnside J, Liou SS, Cobburn LA. 1991. Molecular cloning of the chicken growth hormone receptor complementary deoxyribonucleic acid:mutation of the gene in sex-linked dwarf chickens. Endocrinology. 128(6):3183-3192. https://doi.org/10.1210/endo-128-6-3183
  3. Burnside J, Liou SS, Zhong C, Cogburn LA. 1992. Abnormal growth hormone receptor gene expression in the sexlinked dwarf chicken. Gen Comp Endocrinol. 88(1): 20-28. https://doi.org/10.1016/0016-6480(92)90190-U
  4. Cormack BL, Chase CC, Olson TA, Elsasser TH, Hammond AC,Welsh TH, Jiang H, Randel RD, Okamura CA, Lucy MC. 2009. A miniature condition in Brahman cattle is associated with a single nucleotide mutation within the growth hormone gene. Domest Anim Endocrinol. 37(2):104-111 https://doi.org/10.1016/j.domaniend.2009.04.001
  5. Eleswarapu S, Gu Z, Jiang H. 2008. Growth hormone regulation of insulin-like growth factor-I gene expression may be mediated by multiple distal signal transducer and activator of transcription 5 binding sites. Endocrinology. 149(5):2230-2240. https://doi.org/10.1210/en.2007-1344
  6. Enright WJ, Prendiville DJ, Spicer LJ, Stricker PR, Moloney AP, Mowles TF, Campbell RM. 1993. Effects of growth hormone-releasing factor and (or) thyrotropinreleasing hormone on growth, feed efficiency, carcass characteristics, and blood hormones and metabolites in beef heifers. J Anim Sci. 71(9):2395-2405. https://doi.org/10.2527/1993.7192395x
  7. Etherton TD, Bauman DE. 1998. Biology of somatotrophin in growth and lactation of domestic animals. Physiol Rev. 78(3):745-761. https://doi.org/10.1152/physrev.1998.78.3.745
  8. Huang N, Cogburn LA, Agarwal SK, Marks HL, Burnside J. 1993. Over expression of a truncated growth hormone receptor in the sex-linked dwarf chicken: evidence for a splice mutation. Mol Endocrinol. 7(11):1391-1398. https://doi.org/10.1210/me.7.11.1391
  9. Kelly PA, Djiane J, Postel-Vinay MC, Edery M. 1991. The prolactin/growth hormone receptor family. Endocr Rev. 12(3):235-251. https://doi.org/10.1210/edrv-12-3-235
  10. Laron ZVI, Pertzelan A, Mannheimer S. 1966. Genetic pituitary dwarfism with high serum concentration of growth hormone; a new inborn error of metabolism. Isr J Med Sci. 2:152-155.
  11. Laron Z, Klinger B. 1994. Laron syndrome: clinical features, molecular pathology and treatment. Horm Res. 42(4-5): 198-202. https://doi.org/10.1159/000184193
  12. Lian L, Wang H, Xu J, Zeng C, Hu W. 1993. Biological characteristics of Banna minipig. Shanghai Lab Anim Sci. 13(4):185-191.
  13. Liu J, Boyd CK, Kobayashi Y, Chase CC, Hammond AC, Olson TA, Elsasser TH, Lucy MC. 1999. A novel phenotype for Laron dwarfism in miniature Bos indicus cattle suggests that the expression of growth hormone receptor 1A in liver is required for normal growth. Domest Anim Endocrinol. 17(4):421-437. https://doi.org/10.1016/S0739-7240(99)00051-X
  14. Lucy MC. 2008. Functional differences in the growth hormone and insulin-like growth factor axis in cattle and pigs: implications for post-partum nutrition and reproduction. Reprod Domest Anim. 43(2):31-39. https://doi.org/10.1111/j.1439-0531.2008.01140.x
  15. Renaville R, Hammadi M, Portetelle D. 2002. Role of the somatotropic axis in the mammalian metabolism. Domest Anim Endocrinol. 23(1-2):351-360. https://doi.org/10.1016/S0739-7240(02)00170-4
  16. Wells JA. 1996. Binding in the growth hormone receptor complex. Proceedings of the National Academy of Sciences of the USA. 93(1):1-6. https://doi.org/10.1073/pnas.93.1.1

피인용 문헌

  1. PRKG2 Splice Site Variant in Dogo Argentino Dogs with Disproportionate Dwarfism vol.12, pp.10, 2011, https://doi.org/10.3390/genes12101489