Research in Plant Disease (식물병연구)

The Korean Society of Plant Pathology (한국식물병리학회)
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Development and Evaluation of PCR-Based Detection for Pseudomonas syrinage pv. tomato in Tomato Seeds

토마토 종자로부터 PCR을 이용한 Pseudomonas syringae pv. tomato의 검출

  • Cho, Jung-Hee (Department of Plant Medicine, Chungbuk National University) ;
  • Yim, Kyu-Ock (Animal, Plant and Fisheries Quarantine and Inspection Agency) ;
  • Lee, Hyok-In (Animal, Plant and Fisheries Quarantine and Inspection Agency) ;
  • Yea, Mi-Chi (Animal, Plant and Fisheries Quarantine and Inspection Agency) ;
  • Cha, Jae-Soon (Department of Plant Medicine, Chungbuk National University)
  • 조정희 (충북대학교 식물의학과) ;
  • 임규옥 (농림수산검역검사본부 식물검역부) ;
  • 이혁인 (농림수산검역검사본부 식물검역부) ;
  • 예미지 (농림수산검역검사본부 식물검역부) ;
  • 차재순 (충북대학교 식물의학과)
  • Received : 2011.11.04
  • Accepted : 2011.11.28
  • Published : 2011.12.31
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Abstract

The bacterial speck of tomato caused by Pseudomonas syringae pv. tomato leads to serious economic losses especially on fruits of susceptible genotype. Thus, Pseudomonas syringae pv. tomato is a plant quarantine bacterium in many countries including Korea. In this study, we developed specific PCR assays for detection of the bacterium from tomato seeds. A specific primer set is designed from the hrpZ gene for specific detection of Pseudomonas syringae pv. tomato. A 501 bp PCR product corresponding to hrpZ gene was amplified only form Pseudomonas syringae pv. tomato strains, but no PCR product was amplified from other tomato bacterial pathogens, such as Pseudomonas syringae pv. glycinea, P. syringae pv. maculicola, P. syringae pv. atropurpurea, P. syringae pv. morsprunorum, and from other P. syringae pathovar strains. The nested-PCR primer set corresponding to an internal fragment of the 501 bp sequence (hrpZ) gine was used to specific detection of Pseudomonas syringae pv. tomato in tomato seed. A 119 bp PCR product using nested PCR primer was highly specific and sensitive to detect low level of Pseudomonas syrigae pv. tomato in tomato seeds. We believe that the PCR assays developed in this study is very useful to detect Pseudomonas syringae pv. tomato from the tomato seeds.

P. syringae pv. tomato는 토마토에서 bacterial speck병을 일으키는 종자전염 세균으로, 감수성 품종에서 주로 발병하여 경제적으로 큰 손실을 입힌다. 따라서 P. syringae pv. tomato는 한국을 비롯한 많은 나라에서 식물 검역대상 세균으로 지정하여 관리되고 있다. 본 연구에서 우리는 토마토 종자로부터 PCR을 이용하여 Pst를 검출할 수 있는 방법을 개발하였다. P. syringae pv. tomato의 hrpZ 유전자에서 특이적인 프라이머를 개발하였다. 개발된 프라이머는 P. syringae pv. tomato에서만 501 bp 크기의 특이적 DNA를 증폭하였으며, P. syringae pv. glycinea, P. syringae pv. maculicola, P. syringae pv. atropurpurea, P. syringae pv. morsprunorum와 같은 다른 토마토 세균병원균과 P. syringae pathovar 균주들에서는 증폭되지 않았다. Nested PCR 프라이머를 이용한 PCR에서도 오직 P. syringae pv. tomato에서만 119 bp 크기의 특이적 DNA가 증폭되었고, 토마토 종자에서 P. syringae pv. tomato을 정확하고 민감하게 검출하였다. 본 연구는 현재까지 사용되고 있는 Pst의 검출방법의 민감도를 비교한 최초의 보고로 본 연구에서 개발된 PCR방법들은 토마토 종자에서 Pst을 검출하는 매우 유용한 방법으로 생각된다.

Keywords

References

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