Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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GUS gene expression and plant regeneration via co-culturing with Agrobacterium in grapevine (Vitis vinifera)

Agrobacterium 공동배양을 이용한 포도 재분화율 향상과 GUS 유전자의 발현

  • Kim, Se-Hee (National Institute of Horticultural & Herbal Science, RDA) ;
  • Kim, Jeong-Hee (National Institute of Horticultural & Herbal Science, RDA) ;
  • Kim, Ki-Ok (National Institute of Horticultural & Herbal Science, RDA) ;
  • Do, Gyeong-Ran (National Institute of Horticultural & Herbal Science, RDA) ;
  • Shin, Il-Sheob (National Institute of Horticultural & Herbal Science, RDA) ;
  • Cho, Kang-Hee (National Institute of Horticultural & Herbal Science, RDA) ;
  • Hwang, Hae-Seong (National Institute of Horticultural & Herbal Science, RDA)
  • 김세희 (농촌진흥청 국립원예특작과학원) ;
  • 김정희 (농촌진흥청 국립원예특작과학원) ;
  • 김기옥 (농촌진흥청 국립원예특작과학원) ;
  • 도경란 (농촌진흥청 국립원예특작과학원) ;
  • 신일섭 (농촌진흥청 국립원예특작과학원) ;
  • 조강희 (농촌진흥청 국립원예특작과학원) ;
  • 황해성 (농촌진흥청 국립원예특작과학원)
  • Received : 2011.12.05
  • Accepted : 2011.12.12
  • Published : 2011.12.31
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Abstract

Efficient transformation and regeneration methods are a priority for successful application of genetic engineering to vegetative propagated plants such as grape. In this study, methods for Agrobacterium tumefaciens-mediated transformation and plant regeneration of grapevine (Vitis vinifera) were evaluated. Tamnara, Heukgoosul, Heukbosek, Rizamat were co-cultivated with Agrobacterium strains, LBA4404 containing the vector pBI121 carrying with CaMV 35S promoter, GUS gene as reporter gene and resistance to kanamycin as selective agent. Seven percent of the maximum regeneration frequency was obtained from co-cultivated with explants from Rizamat with LBA4404 strain on selection medium with kanamycin. The addition of acetosyringone, 200 ${\mu}m$ in virulence induction step was a key factor for successful GUS reporter gene expression in grapevine transformation. Transgenic plants showed resistance to kanamycin and the GUS positive response in leaf ($T_0$) stem ($T_0$) and petiole ($T_0$).

Keywords

References

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