Journal of the Korean Magnetic Resonance Society (한국자기공명학회논문지)

Korean Magnetic Resonance Society (한국자기공명학회)
권호 표지
DOI QR코드

DOI QR Code

The Status of Guanine Nucleotides in Taxol-Stabilized Microtubules Probed by 31P CPMAS NMR Spectroscopy

  • Received : 2011.11.05
  • Accepted : 2011.12.13
  • Published : 2011.12.20
Download PDF
⟨ Previous Next ⟩

Abstract

Rapid exchange and hydrolysis of the tubulin-bound guanine nucleotides have been known to govern the dynamics of microtubules. However, the instability and low concentration have made it difficult for the microtubule-bound GTP to be observed directly. In this study, we circumvent these problems by lyophilization and using cross-polarization techniques. $^{31}P$ NMR signals were detected from the tubulin-bound GTP in microtubules for the first time. Analysis of the $^{31}P$ CPMAS NMR spectrum indicates that GTP hydrolysis was delayed by the presence of taxol.

Keywords

References

  1. A. Desai, T. J. Michison, Annu. Rev. Cell Dev. Biol. 13, 83, (1997). https://doi.org/10.1146/annurev.cellbio.13.1.83
  2. R. D. Vale, R. A. Milligan, Science 288, 88, (2000). https://doi.org/10.1126/science.288.5463.88
  3. C. A. Athale, A. Dinarina, M. Mora-Coral, C. Pugieux, F. Nedelec, E. Karsenti, Science 322, 1243, (2008). https://doi.org/10.1126/science.1161820
  4. A. Akhmanova, C. C. Hoogenraad, Curr. Opin. Cell Biol. 17, 47, (2005). https://doi.org/10.1016/j.ceb.2004.11.001
  5. R. Dixit, J. L. Ross, Y. E. Goldman, L. F. Holzbaur, Science 319, 1086, (2008). https://doi.org/10.1126/science.1152993
  6. S. S. Goodwin, R. D. Vale, Cell. 143, 263, (2010). https://doi.org/10.1016/j.cell.2010.09.022
  7. L. A. Amos, T. S. Baker, Nature 279, 607, (1979). https://doi.org/10.1038/279607a0
  8. E. Nogales, S. G. Wolf, K. H. Downing, Nature 391, 199, (1998). https://doi.org/10.1038/34465
  9. E. Nogales, M. Whittaker, R. A. Milligan, K. H. Downing, Cell. 96, 79, (1999). https://doi.org/10.1016/S0092-8674(00)80961-7
  10. H. P. Erickson, E. T. O'Brien, Annu. Rev. Biophys. Biomol. Struct. 21, 145, (1992). https://doi.org/10.1146/annurev.bb.21.060192.001045
  11. A. Dimitrov, M. Quesnoit, S. Moutel, I. Cantaloube, C. Pous, F. Perez, Science 322, 1353, (2008). https://doi.org/10.1126/science.1165401
  12. H. Y. Kueh, T. J. Mitchison, Science 325, 960, (2009). https://doi.org/10.1126/science.1168823
  13. M. Geyer, T. Schweins, C. Hermann, T. Prisner, A. Wittinghofer, H. R. Kalbitzer, Biochem. 35, 10308, (1996). https://doi.org/10.1021/bi952858k
  14. M. Spoemer, T. Prisner, A. E. Bennett, M. M. Hertel, N. Weiden, T. Schweins, H. R. Kalbitzer, Magn. Reson. Chem. 43, S74, (2005). https://doi.org/10.1002/mrc.1693
  15. P. Rosch, A. Wittinghofer, J. Tucker, G. Sczakiel, R. Leberman, I. Schlichting, Biochem. Biophys. Res. Commun. 135, 549, (1986). https://doi.org/10.1016/0006-291X(86)90029-X
  16. A. Nawrotek, M. Knossow, B. Gigant, J. Mol. Biol. 412, 35, (2011). https://doi.org/10.1016/j.jmb.2011.07.029
  17. G.-A. Gang, S.-S. Choi, T.-J. Park, Y. Kim, J. Kor. Magn. Reson. Soc. 14, 88, (2010). https://doi.org/10.6564/JKMRS.2010.14.2.088
  18. T.-J. Park, M.-H. Lee, S.-S. Choi, Y. Kim, J. Kor. Magn. Reson. Soc. 15, 25, (2011). https://doi.org/10.6564/JKMRS.2011.15.1.025
  19. C. Elie-Caille, F. Severin, J. Helenius, J. Howard, D. J. Muller, A. A. Hyman, Curr. Biol. 17, 1765, (2007). https://doi.org/10.1016/j.cub.2007.08.063

Cited by

  1. Solid-state31P NMR investigation on the status of guanine nucleotides in paclitaxel-stabilized microtubules vol.53, pp.5, 2015, https://doi.org/10.1002/mrc.4183