BMB Reports

생화학분자생물학회 (Korean Society for Biochemistry and Molecular Biology)
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Regulatory roles of ganglioside GQ1b in neuronal cell differentiation of mouse embryonic stem cells

  • Kwak, Dong-Hoon (Center for Herbal Medicine Improvement Research, Korea Institute of Oriental Medicine) ;
  • Jin, Jung-Woo (Department of Biological Science, College of Natural Sciences, Wonkwang University) ;
  • Ryu, Jae-Sung (Department of Biological Science, College of Natural Sciences, Wonkwang University) ;
  • Ko, Kinram (Department of Neuroscience, School of Medicine, Konkuk University) ;
  • Lee, So-Dam (Department of Biological Science, College of Natural Sciences, Wonkwang University) ;
  • Lee, Jeong-Woong (Center for Development and Differentiation, Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Kim, Ji-Su (National Primate Research Center, Korea Institute of Bioscience and Biotechnology) ;
  • Jung, Kyu-Yong (Department of Pharmacology, Wonkwang University School of Medicine) ;
  • Ko, Ki-Sung (Department of Biological Science, College of Natural Sciences, Wonkwang University) ;
  • Ma, Jin-Yeul (Center for Herbal Medicine Improvement Research, Korea Institute of Oriental Medicine) ;
  • Hwang, Kyung-A (Department of Agrofood Resources, National Academy of Agricultural Science, RDA) ;
  • Chang, Kyu-Tae (National Primate Research Center, Korea Institute of Bioscience and Biotechnology) ;
  • Choo, Young-Kug (Department of Biological Science, College of Natural Sciences, Wonkwang University)
  • 투고 : 2011.08.11
  • 심사 : 2011.10.04
  • 발행 : 2011.12.31
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초록

Gangliosides play an important role in neuronal differentiation processes. The regulation of ganglioside levels is related to the induction of neuronal cell differentiation. In this study, the ST8Sia5 gene was transfected into mESCs and then differentiated into neuronal cells. Interestingly, ST8Sia5 gene transfected mESCs expressed GQ1b by HPTLC and immunofluorescence analysis. To investigate the effects of GQ1b over-expression in neurogenesis, neuronal cells were differentiated from GQ1b expressing mESCs in the presence of retinoic acid. In GQ1b expressing mESCs, increased EBs formation was observed. After 4 days, EBs were co-localized with GQ1b and nestin, and GFAP. Moreover, GQ1b co-localized with MAP-2 expressing cells in GQ1b expressing mESCs in 7-day-old EBs. Furthermore, GQ1b expressing mESCs increased the ERK1/2 MAP kinase pathway. These results suggest that the ST8Sia5 gene increases ganglioside GQ1b and improves neuronal differentiation via the ERK1/2 MAP kinase pathway.

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참고문헌

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피인용 문헌

  1. Ganglioside GM1 influences the proliferation rate of mouse induced pluripotent stem cells vol.45, pp.12, 2012, https://doi.org/10.5483/BMBRep.2012.45.12.138
  2. Roles of gangliosides in the differentiation of mouse pluripotent stem cells to neural stem cells and neural cells vol.16, pp.2, 2017, https://doi.org/10.3892/mmr.2017.6719