DOI QR코드

DOI QR Code

The Proliferative and Apoptotic Properties of Nile Tilapia (Oreochromis niloticus) sIg+ Lymphocytes by Cortisol Treatment

  • Park, Kwan-Ha (Department of Aquatic Life Medicine, Kunsan National University) ;
  • Choi, Sang-Hoon (Department of Aquatic Life Medicine, Kunsan National University)
  • Received : 2011.10.14
  • Accepted : 2011.12.15
  • Published : 2011.12.31

Abstract

The effects of cortisol on proliferation and apoptosis of tilapia surface immunoglobulin positive ($sIg^+$) lymphocytes isolated from different tissues were investigated. $sIg^+$ lymphocytes from the tilapia head kidney (HK) and spleen showed a higher proliferation and lower intracellular calcium ($Ca^{2+}{_i}$) level to Ig-crosslinking compared with peripheral blood $sIg^+$ lymphocytes. Peripheral blood $sIg^+$ lymphocytes stimulated with lipopolysaccharide (LPS) showed high levels of apoptosis in the presence of cortisol. HK and to a lesser extent spleen $sIg^+$ lymphocytes, although less sensitive than their equivalent in peripheral blood, showed cortisol-induced apoptosis irrespective of LPS stimulation of control levels. Compared to plasma values measured during stress conditions, proliferation regardless of LPS stimulation was apparently suppressed by cortisol that is effective in inducing a significant increase in apoptosis in all three different cell populations of $sIg^+$ cells, suggesting the immunoregulatory effect of cortisol in both LPS stimulated and non-stimulated conditions. Different sensitivity of $sIg^+$ cells to the cortisol, in regard to developmental stage and activity, could be related in inhibiting excessive and continuing depletion of $sIg^+$ lymphocytes.

Keywords

References

  1. Aluru, N. and Vijayan MM. 2009. Stress transcriptomics in fish: a role for genomic cortisol signaling. Gen Comp Endocrinol 164:142-150. https://doi.org/10.1016/j.ygcen.2009.03.020
  2. Ashwell, JD., King, LB. and Vacchio, MS. 1996. Cross-talk between the T cell antigen recetor and the glucocorticoid receptor regulated thymocyte development. Stem Cells 14: 490-500. https://doi.org/10.1002/stem.140490
  3. Barton, BA. and Iwama, GK. 1991. Physiological changes in fish from stress in aquaculture with emphasis on the response and effects of corticosteroids. Ann Rev of Fish Disease 1:3-26. https://doi.org/10.1016/0959-8030(91)90019-G
  4. Bury, NR., Sturm, A., Le Rouzic, P., Lethimonier, C., Ducouret, B. and Guiguen, Y. 2003. Evidence for two distinct functional glucocorticoid receptors in teleost fish. J Mol Endocrinol 31:141-156. https://doi.org/10.1677/jme.0.0310141
  5. Carlson, RE., Anderson, DP., and Bodammer, JE. 1993. In vivo cortisol administration suppresses the in vitro primary immune response of winter tilapia lymphocytes. Fish Shellfish Immunol 3:299-312. https://doi.org/10.1006/fsim.1993.1029
  6. Castro, R., Zou, J., Secombes, CJ. and Martin, SAM. 2011. Cortisol modulates the induction of inflammatory gene expression in a rainbow trout macrophage cell line. Fish and Shellfish Immunol 30:215-223. https://doi.org/10.1016/j.fsi.2010.10.010
  7. Ellis, AE. 1981. Stress and the modulation of defense mechanism in fish. In: Pickering AD (ed), Stress in Fish, Academic Press, London, pp 45-71.
  8. Ellsaesser, CF. and Clem, LW. 1987. Cortisol-induced hematologic and immunologic changes in channel catfish (Ictalurus punctatus). Comp Biochem Physiol A. 87:405-408. https://doi.org/10.1016/0300-9629(87)90143-5
  9. Espelid, S., Lokken, GB., Steiro, K., and Bogwald, J. 1996. Effects of cortisol and stress on the immune system in Atlantic salmon (Salmo salar L.). Fish Shellfish Immunol 6:95-110. https://doi.org/10.1006/fsim.1996.0011
  10. Grimm, AS. 1985. Suppression by cortisol of the mitogen-induced proliferation of peripheral blood leukocytes from plaice, Pleuronectes platessa L. In: Manning MJ and Tatner, MF (ed), Fish Immunology, Academic Press, London, pp 263-271.
  11. Jang, HN., Woo, JK., Cho, YH., Kyong, SB. and Choi, SH. 2004. Characterization of monoclonal antibodies against heavy and light chains of flounder (Paralichthys olivaceus) immunoglobulin. J Biochem Mol Biol 37:314-319. https://doi.org/10.5483/BMBRep.2004.37.3.314
  12. Koumans-van Diepen, JCE., Harmsen, EGM. and Rombout, JHWM. 1994. Immunocytochemical analysis of mitogen responses of carp (Cyprinus carpio L.) peripheral blood leukocytes. Vet Immunol Immunopathol 42:209-219. https://doi.org/10.1016/0165-2427(94)90009-4
  13. Laing, KJ., Holland, J., Bonilla, S., Cunningham, C. and Secombes, CJ. 2001. Cloning and sequencing of caspase 6 in rainbow trout, Oncorhynchus mykiss, and analysis of its expression under conditions known to induce apoptosis. Dev Comp Immunol 25:303-312. https://doi.org/10.1016/S0145-305X(00)00061-6
  14. Lenardo, MJ. 1997. Introduction: The molecular regulation of lymphocyte apoptosis. Seminars in Immunol 9:1-5. https://doi.org/10.1006/smim.1996.0050
  15. Le Morvan-Rocher, C., Troutead, D. and Deschaux, P. 1995 Effects of temperature on carp leukocyte mitogen-induced proliferation and nonspecific cytotoxic activity. Dev Comp Immunol 19:87-95. https://doi.org/10.1016/0145-305X(94)00057-M
  16. Mazur, CF. and Iwama, GK. 1993. Handling and crowding stress reduces number of plaque-forming cells in Atlantic salmon. J Aquatic Animal Health 5:98-101. https://doi.org/10.1577/1548-8667(1993)005<0098:HACSRN>2.3.CO;2
  17. Rombout, JHWM., Egberts, E., Taverne, N., and van Diepen, JCE. 1990. Analysis of the immune system of fish with monoclonal antibodies. In: Schots A (ed), Monoclonal Antibodies in Agriculture, Wageningen, Pudoc, pp 81-86.
  18. Saha, NR., Usami, T. and Suzuki, Y. 2003. A double staining flow cytometric assay for the detection of steroid induced apoptotic leucocytes in common carp (Cyprinus carpio). Dev Comp Immunol 27:351-363. https://doi.org/10.1016/S0145-305X(02)00116-7
  19. Salonius, K. and Iwama, GK. 1993 Effect of early rearing environment on stress response, immune function, and disease resistance in juvenile coho (Oncorhynchus Kisutch) and chinook salmon (O. tshawytscha). Canadian J Aquatic Science 50:759- 766. https://doi.org/10.1139/f93-087
  20. Secombes, CJ., van Groningen, JJM. and Egberts, E. 1983. Separation of lymphocyte subpopulations in carp by monoclonal antibodies: immunocytochemical studies. Immunology 48:165-175.
  21. Tripp, RA., Maule, AG., Schreck, CB. and Kaattari, SL. 1987. Cortisol mediated suppression of salmonid lymphocyte responses in vitro. Devel Comp Immunol 11:565-576. https://doi.org/10.1016/0145-305X(87)90045-0
  22. Verburg-van Kemenade, BML., Saeij, JPJ., Flik, G. and Willems, PHGM. 1998. $Ca^{2+}$ signals during early lymphocyte activation in carp Cyprinus carpio L. J Exp Biol 201:591-598.
  23. Wechsler, SJ., McAllister, PE., Hetrick, FM. and Anderson, DP. 1986. Effect of exogenous corticosteroids on circulating virus and neutralizing antibodies in striped bass (Morone saxatilis) infected with infectious pancreatic necrosis virus. Vet Immunol Immunopathol 12:305-311. https://doi.org/10.1016/0165-2427(86)90134-0
  24. Wendelaar Bonga, SE. 1997. The stress response in fish. Physiol Rev 77:591-625. https://doi.org/10.1152/physrev.1997.77.3.591
  25. Weyts, FAA., Verburg-van Kemenade, BML., Flik, G., Lambert, JGD. and Wendelaar Bonga, SE. 1997. Conservation of apoptosis as an immune regulatory mechanism: effects of cortisol and cortisone on carp lymphocytes. Brain Behav Imm 11:95-105. https://doi.org/10.1006/brbi.1997.0484
  26. Weyts, FAA., Verburg-van Kemenade, BML. and Flik G. 1998a. Characterization of corticoid receptors in peripheral blood leukocytes of carp, Cyprinus carpio L. General Comp Endocrin 111:1-8. https://doi.org/10.1006/gcen.1998.7080
  27. Weyts, FAA., Flik, G., Rombout, JHWM. and Verburg-van Kemenade, BML. 1998b. Cortisol induces apoptosis in activated B cells, but not in thrombocytes or T cells of common carp, Cyprinus carpio L. Dev Comp Immunol 22: 551-562. https://doi.org/10.1016/S0145-305X(98)00033-0
  28. Weyts, FAA., Flik, G., Rombout, JHWM. and Verburg-van Kemenade, BML. 1998c. Cortisol inhibits apoptosis in carp neutrophilic granulocytes. Dev Comp Immunol 22:563-572. https://doi.org/10.1016/S0145-305X(98)00027-5
  29. Weyts, FAA., Cohen, N., Flik, G. and Verburg-van Kemenade, BML. 1999. Interactions between the immune system and the hypothalamo-pituitary-interrenal axis in fish. Fish Shellfish Immunol 9:1-20. https://doi.org/10.1006/fsim.1998.0170
  30. Yada, T. and Nakanishi, T. 2002. Interaction between endocrine and immune systems in fish. Int Rev Cytol 220:35-92. https://doi.org/10.1016/S0074-7696(02)20003-0