Journal of Pharmaceutical Investigation

The Korean Society of Pharmaceutical Sciences and Technology (한국약제학회)
권호 표지
DOI QR코드

DOI QR Code

Protective Effects of Acorn (Quercus acutissima CARR.) against IgE-mediated Allergic and Ovalbumin (OVA)-Induced Asthmatic Responses via Inhibition of Oxidative Stress

  • Chung, Mi-Ja (Department of Biotechnology, The Catholic University of Korea) ;
  • Jo, Hang-Soo (Department of Biotechnology, The Catholic University of Korea) ;
  • Choi, Ha-Na (Department of Biotechnology, The Catholic University of Korea) ;
  • Cho, Soo-Muk (Department of Korean Food Research for Globalization, National Academy of Agricultural Science, Rural Development Administration (RDA)) ;
  • Park, Yong-Il (Department of Biotechnology, The Catholic University of Korea)
  • Received : 2011.11.30
  • Accepted : 2011.12.03
  • Published : 2011.12.20
Download PDF
⟨ Previous Next ⟩

Abstract

This work was performed to investigate the protective effect of ethanol extract (AEx) from acorn (Quercus acutissima CARR.) against allergic mediated responses in asthma model cells and mice. The AEx inhibited antigen-stimulated cytokine production such as interleukin (IL)-4, IL-13 and tumor necrosis factor-${\alpha}$ (TNF-${\alpha}$) and AEx also inhibited intracellular reactive oxygen species (ROS) generation against IgE-mediated allergic response in rat basophilic leukaemia RBL-2H3 cells. The ovalbumin (OVA)-sensitized mice were orally administered with AEx (100 or 300 mg/kg) and authentic tannic acid (75 mg/kg) every day for 15 days. Increased TNF-${\alpha}$ production by OVA-sensitization/challenge was significantly reduced by administration of AEx. The serum triglyceride levels of asthma mice were significantly reduced after feeding for 15 days with tannic acid or AEx. The mice fed with tannic acid or AEx also exhibited a significant reduction in body weights compared to those of asthma control group. The AEx increased the heme oxygenase (HO)-1 mRNA expression in the asthma model mice and showed DPPH radical scavenging activity. These results indicate that AEx protects against IgEmediated allergic and OVA-induced asthmatic responses via direct and indirect antioxidant activities. Reduced triglyceride and body weights may provide additional protective benefits of AEx on allergic asthma.

Keywords

References

  1. Busse, W.W., Rosenwasser, L.J., 2003. Mechanisms of asthma. J. Allergy Clin. Immun. 111, S799-S804. https://doi.org/10.1067/mai.2003.158
  2. Choi, Y.A., Kim, D.K., Chun, M.K., Choi, H.K., Lee, Y.M., 2009. Comparison of oral versus rectal administration of processedscutellaria baicalesis on colonic inflammation in mice. J. Pharm. Invest. 39, 381-386. https://doi.org/10.4333/KPS.2009.39.5.381
  3. Choi, H.J., Chung, M.J., Ham, S.S., 2010. Antiobese and hypocholesterolaemic effects of an Adenophora triphylla extract in HepG2 cells and high fat diet-induced obese mice. Food Chem. 119, 437-444. https://doi.org/10.1016/j.foodchem.2009.06.039
  4. Chung, M.J., Walker, P.A., Brown, R.W., Hogstrand, C., 2005. ZINC-mediated gene expression offers protection against $H_{2}O_{2}$-induced cytotoxicity. Toxicol. Appl. Pharm. 205, 225- 236. https://doi.org/10.1016/j.taap.2004.10.008
  5. Foster, P.S., Hogan, S.T., Ramsay, A.J., Matthaei, K.I., Young, I.G., 1996. Interleukin-5 deficiency abolishes eosinophilia, airways hyperreactivity, and lung damage in a mouse asthma model. J. Exp. Med. 183, 195-201. https://doi.org/10.1084/jem.183.1.195
  6. Figueroa-Munoz, J.I., Chinn, S., Rona, R.J., 2001. Association between obesity and asthma in 4-11 year old children in the UK. Thorax 56, 133-137. https://doi.org/10.1136/thorax.56.2.133
  7. Gulcin, I., Huyut, Z., Elmastas, M., Aboul-Enein, H.Y., 2010. Radical scavenging and antioxidant activity of tannic acid. Arab. J. Chem. 3, 43-53. https://doi.org/10.1016/j.arabjc.2009.12.008
  8. Huang, F., Yamaki, K., Tong, X., Fu, L., Zhang, R., Cai, Y., Yanagisawa, R., Inone, K.I., Takano, H., Yoshino, S., 2008. Inhibition of the antigen-induced activation of RBL-2H3 cells by sinomenine. Int. Immunopharmacol. 8, 502-507. https://doi.org/10.1016/j.intimp.2007.12.009
  9. Han, E.H., Hwang, Y.P., Kim, H.G., Park, J.H., Choi, J.H., Im, J.H., Khanal, T., Park, B.H., Yang, J.H., Choi, J.M., Chun, S.S., Seo, J.K., Chung, Y.C., Jeong, H.G., 2011. Ethyl acetate extract of Psidium guajava inhibits IgE-mediated allergic responses by blocking FcRI signaling. Food Chem. Toxicol. 49, 100-108. https://doi.org/10.1016/j.fct.2010.10.003
  10. Itoh, T., Ohguchi, K., Nakajima, C., Oyama, M., Iinuma, M., Nozawa, Y., Akao, Y., Ito, M., 2011. Inhibitory effects of flavonoid glycosides isolated from the peel of Japanese persimmon (Diospyros kaki Fuyu) on antigen-stimulated degranulation in rat basophilic leukaemia RBL-2H3 cells. Food Chem. 126, 289-294. https://doi.org/10.1016/j.foodchem.2010.10.058
  11. Itoh, T., Ohguchi, K., Iinuma, M., Nozawa, Y., Akao, Y., 2008. Inhibitory effect of xanthones isolated from the pericarp of Garcinia mangostana L. on rat basophilic leukemia RBL-2H3 cell degranulation. Bioorgan. Med. Chem. 16, 4500-4508. https://doi.org/10.1016/j.bmc.2008.02.054
  12. Kang, M.H., Lee, J.H., Lee, J.S., Kim, J.H., Chung, H.K., 2004. Effects of acorn supplementation on lipid profile and antioxidant enzyme activities in high fat diet-induced obese rats. Korean J. Nutr. 37, 169-175.
  13. Lee, M.Y., Seo, C.S., Ha, H.K., Jung, D.Y., Lee, H.Y., Lee, N.H., Lee, J.A., Kim, J.H., Lee, Y.K., Son, J.K., Shin, H.K., 2010. Protective effects of Ulmus davidiana var. japonica against OVA-induced murine asthma model via upregulation of heme oxygenase-1. J Ethnopharmacol. 130, 61-69. https://doi.org/10.1016/j.jep.2010.04.011
  14. Lee, S.H., Kim, D.I., Cho, S.Y., Jung, H.J., Cho, S.M., Park, H.J., Lillehoj, H.S., 2005. Effects of Acorn (Quercus acutissima CARR.) supplementation on the level of acethylcholine and its related enzyme activities in the brain of dementia mouse model. J. Korean Soc. Food Sci. Nutr. 34, 738-742. https://doi.org/10.3746/jkfn.2005.34.5.738
  15. Lee, S.H., Bae, E.A., Park, E.K., Shin, Y.W., Baek, N.I., Han, E.J., Chung, H.G., Kim, D.H., 2007. Inhibitory effect of eupatilin and jaceosidin isolated from Artemisia princeps in IgEinduced hypersensitivity. Int. Immunopharmacol. 7, 1678- 1684. https://doi.org/10.1016/j.intimp.2007.08.028
  16. Lee, M.H., Jeong, J.H., Oh, M.J., 1992. Antioxidative activity of gallic acid in Acorn extract. J. Korean Soc. Food Sci. Nutr. 21, 693-700.
  17. Mosmann, T.R., Sad, S., 1996. The expanding universe of T-cell subsets: Th1, Th2 and more. Immunol. Today 17, 138-146. https://doi.org/10.1016/0167-5699(96)80606-2
  18. Pae, H.O., Oh, G.S., Choi, B.M., Chae, S.C., Chung, H.T., 2003. Differential expressions of heme oxygenase-1 gene in CD25- and $CD25^{+}$ subsets of human $CD4^{+}$ T cells. Biochem. Bioph. Res.Co. 306, 701-705. https://doi.org/10.1016/S0006-291X(03)01037-4
  19. Park, S.J., Shin, W.H., Seo, J.W., Kim, E.J., 2007. Anthocyanins inhibit airway inflammation and hyperresponsiveness in a murine asthma model. Food Chem. Toxicol. 45, 1459-1467. https://doi.org/10.1016/j.fct.2007.02.013
  20. Springer, J., Groneberg, D.A., Dinh, Q.T., Quarcoo, D., Hamelmann, E., Braun-Dullaeus, R.C., Geppetti, P., Anker, S.D., Fischer A., 2007. Neurokinin-1 receptor activation induces reactive oxygen species and epithelial damage in allergic airway inflammation. Clin. Exp. Allergy 37, 1788-1797. https://doi.org/10.1111/j.1365-2222.2007.02851.x
  21. Szabo, S.J., Sullivan, B.M., Stemmann, C., Satoskar, A.R., Sleckman, B.P., Glimcher, L.H., 2002. Distinct effects of T-bet in TH1 lineage commitment and INF-gamma production in CD4 and CD8 T cells. Science 295, 338-342. https://doi.org/10.1126/science.1065543
  22. Shore, S.A., 2007. Obesity and asthma: lessons from animal models. J. Appl. Physiol. 102, 516-528. https://doi.org/10.1152/japplphysiol.00847.2006
  23. Shim, T.H., Jin, Y.S., Sa, J.H., Shin, I.C., Heo, S.I., Wang, M.H., 2004. Studies for component analysis and antioxidant evaluation in Acorn Powders. Korean J. Food Sci. Technol. 36, 800-803.
  24. Tejerina, D., Garcia-Torres, S., Cabeza De Vaca, M., Vazquez, F.M., Cava, R., 2011. Acorns (Quercus rotundifolia Lam.) and grass as natural source of antioxidants and fatty acids in the "montanera" feeding of Iberian pig: Intra- and inter-annual variations. Food Chem. 124, 997-1004. https://doi.org/10.1016/j.foodchem.2010.07.058
  25. Williams, C.M., Galli, S.J., 2000. Mast cells can amplify airway reactivity and features of chronic inflammation in an asthma model in mice. J. Exp. Med. 192, 455-462. https://doi.org/10.1084/jem.192.3.455