Korean Journal of Breeding Science (한국육종학회지)

The Korean Society of Breeding Science (한국육종학회)
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Identification of Leaf Blast Resistance Genes Derived from a Korean Weedy Rice, Ganghwaaengmi 11

잡초성벼인 강화앵미11 유래 잎도열병 저항성 유전자 탐색

  • 서정필 (농촌진흥청 국립식량과학원) ;
  • 조영찬 (농촌진흥청 국립식량과학원) ;
  • 김정주 (농촌진흥청 국립식량과학원) ;
  • 신영섭 (농촌진흥청 국립식량과학원) ;
  • 양창인 (농촌진흥청 국립식량과학원) ;
  • 노재환 (농촌진흥청 국립식량과학원) ;
  • 김연규 (농촌진흥청 국립식량과학원)
  • Received : 2010.09.01
  • Published : 2010.09.30
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Abstract

A weedy rice, Ganghwaaengmi 11, shows high level of leaf blast resistance. The chromosomal number and locations of genes conferring the leaf blast resistance were detected by QTL (quantitative trait loci) analysis using SSR markers in the 120 RILs (recombinant inbred lines) derived from the cross between Nagdongbyeo and Ganghwaaengmi 11. Ganghwaaengmi 11 expressed compatibility with 20 of the 45 inoculated blast isolates, in contrast to Nagdongbyeo with 44 compatible isolates. To identify QTLs affecting partial resistance, RILs were assessed in upland blast nursery in three regions and inoculated with selected nine blast isolates. QTLs for resistance to blast isolates were identified on chromosomes 7, 11 and 12. Three QTLs associated with blast resistance in nursery test at three regions were also detected on chromosomes 7, 11 and 12. The QTL commonly detected on chromosome 12 was only increased blast resistance by Ganghwaaengmi 11 allele. This QTL accounted for 60.3~78.6% of the phenotypic variation in the blast nursery test. OSR32 and RM101 markers tightly linked to QTL for blast resistance on chromosome 12 might be useful for marker-assisted selection (MAS) and gene pyramiding to improve the blast resistance of japonica rice.

본 연구는 국내 수집 잡초성벼에 존재하는 잎도열병 저항성 관련 유전자를 탐색하고, 이 저항성유전자와 연관된 분자 마커를 탐색하는 것이다. 도열병에 감수성인 자포니카 품종인 낙동벼와 도열병에 강한 잡초성벼인 강화앵미11을 교잡하여 120개 RILs를 육성하여, 도열병 균주반응과 잎도열병 밭못자리검정을 통한 저항성 유전자 탐색에 이용하였다. 1. 총 45개 도열병 균주를 이용하여 양친들을 검정한 결과, 잡초성벼 강화앵미11은 25개 균주에 대하여 저항성 반응을 보였고, 낙동벼는 1개의 균주에만 저항성 반응을 보였다. 2. 강화앵미11이 저항성반응을 보이는 25개 균주 중에서 90-008등 9개 균주를 선발하여 120개 RILs에 대한 도열병 균주에 대한 저항성반응을 조사한 결과, 대부분의 RILs이 저항성반응으로 치우친 경향을 보였으며, 수원, 철원 및 남양 잎도열병 밭못자리 검정포장에서 실시한 120 RILs의 저항성반응 분포도 도열병 균주반응에서 나타난 결과와 유사한 경향을 보였다. 3. 12번 염색체 OSR32-RM101 영역에서 90-008, 97-268, 93-456, 04-226, 03-177 및 87-138 도열병 균주와 수원, 철원, 남양 지역 잎도열병 밭못자리 검정에 대한 저항성 유전자를 탐색하였으며, 이 저항성 유전자는 강화앵미11에서 유래되었고, 표현형변이를 60%이상 설명하는 주동유전자인 것으로 추정된다. 4. 90-008균주와 남양 잎도열병 밭못자리 검정에 대한 저항성 유전자는 12번 염색체 외에 11번 염색체에서도 탐색되었으며, 93-456 균주와 철원, 수원지역 잎도열병 밭못자리 검정에 대한 저항성유전자는 7번과 11번 염색체에서도 탐색되었고, 이들 저항성유전자는 낙동벼 대립유전자에 의해 저항성이 증가되었다.

Keywords

Acknowledgement

Supported by : 농촌진흥청

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