Korean Journal of Breeding Science (한국육종학회지)

The Korean Society of Breeding Science (한국육종학회)
권호 표지

Phylogenetic analysis and association of markers and traits related to starch contents in Korean potato cultivars using SSRs

  • Received : 2010.03.15
  • Published : 20100300
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Abstract

Diversity of 30 Korean potato cultivars was evaluated using 14 microsatellite markers. Twelve microsatellite markers representing 12 loci in the potato genome detected 84 polymorphisms among 30 cultivars and revealed alleles with a mean of 7.00 alleles per primer. The polymorphism information content (PIC) value ranged from 0.57 to 0.93 with average of 0.82. Based on polymorphism, cluster analysis was conducted by the unweighted pair-group method with arithmetic average (UPGMA) methods. Thirty potato varieties were distinctly separated into 2 groups and similarity coefficient of cluster ranged from 0.58 to 0.95. Thirty tetraploid cultivars were evaluated for six important agronomic traits. One-way analysis of variance was done to look for the degree of relationships between individual markers and traits. K1 and K2 markers showed a significant association with amylose contents, starch contents, and specific gravity.

Keywords

References

  1. Anderson JA, Churchill GA, Autriguc JE, Tanksley SD, 1993. Optimizing parental selection for genetic linkage maps. Genome 36:181-186.
  2. Ashkenazi V, Chani E, Lavi U, Levy D, Hillel J, Veilleux RE. 2001. Development of microsatellite markers in potato and their use in phylogenetic fingerprinting analyses. Genome 44:50-62. https://doi.org/10.1139/gen-44-1-50
  3. Bachem CW, van der Hoeven RS, de Bruijn SM, Vreugdenhil D, Zabeau M, Visser RG. 1996. Visualization of differential gene expression using a novel method of RNA fingerprinting based on AFLP: analysis of gene expression during potato tuber development. Plant J 9:745-753.
  4. Ghislain M, Spooner DM, Rodriguez, Villamon F, Nunez J, Vasquez, Waugh R, Bonierbale M. 2004. Selection of highly informative and user friendly microsatellite (SSRs) for genotyping of cultivated potato. Theor Appl Genet 108:881-890. https://doi.org/10.1007/s00122-003-1494-7
  5. Hutchinson C, White J, Gergcla D, Sohmo P. Haynes K, Wenrich R, Lipp C. 2003. Performance of chip processing potato clones in Northeast Florida. Hort Tech 13:706-711.
  6. Jackson SD. 1999. Multiple signaling pathways control tuber induction in potato. Plant Physiol 119:1-8. https://doi.org/10.1104/pp.119.1.1
  7. Kawchuk LM, Lynch DR, Thomas J. Penner B, Sillito D, Kulcsar F. 1996. Characterization of Solanum tuberosum simple sequence repeats and application to potato cultivar identification. American Potato Journal 73:325-335. https://doi.org/10.1007/BF02849164
  8. Kim KS, Park YE, Cho HM, Yi JY, Hahm YI, Shin KY, Ahn YK, Son DM, Noh JH. 2003. A new early-maturing potato cultivar for chip processing 'Gawon'. Kor J Breed 35:343-344.
  9. Kim SY, Park YE, Seo HW, Yi JY, Cho JH, Park CS, Jang DC, Yoon YH, Ryu KY, Kwon M, Kim JS, Lee YG, Chean JO 2003. Cultivation technique. In: Kim YO (ed.) Potato Cultivation. Sammi, Suwon, pp. 150-186.
  10. McGregor CE, Lambert CA, Greyling MM, Louw JH, Wamich L. 2000. A comparative assessment of DNA fingerprinting techniques (RAPD, ISSR, AFLP. and SSR) in tetfl.lploid potato (Solanum tuberosum L.) germplasm. Euphytica 113:135-144. https://doi.org/10.1023/A:1003925620546
  11. Mitchell SE, Kresovich S, Jester CA, Hemandez CJ, Szewe-McFadden AK. 1997. Application of multiplex PCR and fluorescence-based. semi-automated allele sizing technology for genotyping plant genetic resources. Crop Sci 37:617-624. https://doi.org/10.2135/cropsci1997.0011183X003700020051x
  12. Milbourne D, Meyer R, Bradshaw JE, Baird E, Bonar N, Provan J, Powell W, Waugh R. 1997. Comparison of PCR based marker systems for the analysis of genetic relationships in cultivated potato. Mol Breed 3:127-136. https://doi.org/10.1023/A:1009633005390
  13. Provan J, Powell W, Waugh R. 1996. Microsatellite analysis of relationships within cultivated potato. Theor Appl Genet 92:1078-1084. https://doi.org/10.1007/BF00224052
  14. Riesmeier JW, Wilimitzer L, Frommer WB. 1994. Evidence for an essential role of the sucrose transporter in phloem loading and assimilate partitioning. EMBO J 13:1-7.
  15. Raker C. Spooner DM. 2002. The Chilean tetraploid cultivated potato. Solanum tuberosum, is distinct from the Andean populations; microsatellite data. Crop Sci 42:1451-1458. https://doi.org/10.2135/cropsci2002.1451
  16. Roessner U, Wagner C, Kopka J, Tretheway RN, Willmitzer L. 2000. Technical advance:simultin대us analysis of metabolites in potato tuber by gas chromatography-mass spectrometry. Plant J 23:131-142. https://doi.org/10.1046/j.1365-313x.2000.00774.x
  17. Schneider K, Douches DS. 1997. Assessment of PCR-based simple sequence repeats to fingerprint North America potato cultivars. Am Potato J 74:149-164. https://doi.org/10.1007/BF02851594
  18. Taylor MA, Arif SA, Kumar A, Davies HV, Scobie LA, Pearce SR, Flavell AJ. 1992. Expression and sequence analysis of cDNAs induced during the early stages of tuberization in different organs of the potato plant. Plant Mol Biol 20:641-651. https://doi.org/10.1007/BF00046449
  19. Veilleux RE, Shen LY, Paz MM. 1995. Analysis of the genetic composition of anther-derived potato by randomly amplified polymorphic DNA and simple sequence repeats. Genome 38:1153-1162. https://doi.org/10.1139/g95-153
  20. Visser RG, Somhorst I, Kuipers GJ, Ruys NJ, Feenstra WJ, Jacobsen E. 1991. Inhibition of the expression of the gene for granule-bound starch synthase in potato by antisense constructs. Mol Gen Genet 225:289-296. https://doi.org/10.1007/BF00269861
  21. Westman AL, Kresovich S. 1998. The potential of cross-taxa simple-sequence repeat (SSR) amplification between Arabidopsis thaliana L. and crop brassicas. Theor Appl Genet 96:272-281. https://doi.org/10.1007/s001220050736