Manufacturing and Characteristics of Fruit Wine from Acanthapanax sessiliflorus

오가피 열매 발효주의 제조 및 특성

  • Choi, Jae-Myoung (Department of Food Science and Technology, Chungbuk National University) ;
  • Kim, Kwang-Yup (Department of Food Science and Technology, Chungbuk National University) ;
  • Lee, Sang-Hwa (Department of Food and Nutrition, Seowon University) ;
  • Ahn, Jun-Bae (Department of Food Service Industry, Seowon University)
  • Received : 2009.11.09
  • Accepted : 2009.11.16
  • Published : 2010.02.28

Abstract

Fruit wine from Acanthopanax sessiliflorus(A. sessiliflorus) including many pharmacological components was manufactured and its functional properties were investigated. The fruit part of A. sessiliflorus contained 75.74${\pm}$0.49%(w/w) moisture, 12.51${\pm}$1.23%(w/w) crude protein, 4.20${\pm}$0.51.%(w/w) crude fat and 5.21${\pm}$1.64%(w/w) crude ash. Minerals of fruit were potassium(12.94${\pm}$0.08 mg/g), calcium(1.53${\pm}$0.06 mg/g) and magnesium(1.12${\pm}$0.05 mg/g). Initial soluble solid and fermentation temperature were 24-30$^{\circ}$Brix and 20${^{\circ}C}$ for manufacturing fruit wine from A. sessiliflorus. When initial soluble solid of a must was adjusted to more than 30$^{\circ}$Brix, ethanol production was suppressed slightly. The polyphenol content of the fruit wine fermented at 20${^{\circ}C}$(125.24${\pm}$1.86 mg/mL) was higher than those at 25${^{\circ}C}$(99.69${\pm}$2.11 mg/mL) and 30${^{\circ}C}$(95.55${\pm}$1.54 mg/mL). Electron donating activities of wines fermented at 20, 25 and 30${^{\circ}C}$ were 85.9${\pm}$2.3, 55.7${\pm}$2.5 and 55.2${\pm}$3.4%, respectively. The content of eleutheroside B increased up to 146.58${\pm}$4.10 $\mu$g/mL during fermentation. There was no significant effect of fermentation temperature on eleutheroside B content. The fruit part of A. sessiliflorus can be used as a valuable resource for development of nutraceutical foods.

본 연구에서는 오가피(Acanthopanax sessiliflorus)의 열매 발효주를 제조하였고 발효 중 생리활성 성분의 함량 변화를 측정함으로써 그 특징을 규명하였다. 열매의 일반성분 조성은 수분75.74${\pm}$0.49%(w/w), 조단백질 12.51${\pm}$1.23%(w/w), 조지방 4.20${\pm}$0.51.%(w/w), 조회분 5.21${\pm}$1.64%(w/w)이었으며 주된 미네랄 성분은 칼륨(12.94${\pm}$0.08 mg/g), 칼슘 (1.53${\pm}$0.06 mg/g), 마그네슘(1.12${\pm}$0.05 mg/g) 등 이었다. 발효주 제조를 위해 설탕으로 24, 26, 28, 30, 32$^{\circ}$Brix가 되도록 가당하고 발효 한 결과 30$^{\circ}$Brix 이상 가당 하였을 경우 알콜 발효가 저해되었으므로 목적하는 최종 알콜 농도와 잔당에 따라 24-30$^{\circ}$Brix가 되도록 조절하는 것이 바람직하였다. 발효가 진행되면서 총 폴리페놀의 함량은 증가하는 경향을 보였고 20${^{\circ}C}$로 발효한 경우가 125.24${\pm}$1.86 mg/mL로 25${^{\circ}C}$(99.69${\pm}$2.11 mg/mL), 30${^{\circ}C}$(95.55${\pm}$1.54 mg/mL)에서 발효한 경우보다 더 많은 총 폴리페놀 함량을 보였다. 또한 전자공여능을 측정 한 결과 20, 25, 30${^{\circ}C}$에서 발효시 각각 85.9${\pm}$2.3, 55.7${\pm}$2.5, 55.2${\pm}$3.4%로 20${^{\circ}C}$에서 발효하였을 경우 가장 높은 전자공여능을 보였는데 이는 1%(w/v) $\alpha$-tocopherol의 전자공여능과 비슷한 수준이었다. 오가피 열매의 주된 생리활성 물질인 eleutheroside B 함량은 발효가 진행됨에 따라 증가하여 최대 146.58${\pm}$4.10 $\mu$g/mL에 이르렀다. 그러나 발효 온도에 따라 eleutheroside B 함량은 차이가 없음을 알 수 있었다. 본 연구를 통해 착색료나 동물사료로 사용되거나 폐기되는 오가피 열매가 생리활성물질을 다량 함유한 고부가가치 기능성 식품 소재로 활용될 수 있는 가능성을 제시하였다.

Keywords

Acknowledgement

Supported by : 서원대학교

References

  1. AOAC. 1985. Official Method of Analysis. 15th ed. Association Official Analytical Chemists, Washingtong D.C, USA. pp.914-915
  2. Blois, MS. 1958. Antioxidant determination by the use of a stable free radical. Nature 181: 1199-1200 https://doi.org/10.1038/1811199a0
  3. Brekhman II, Dardmov IV. 1969. New substances of plant origin which increase nonspecific resistance. Annu. Rev. Pharmacolog. 9: 419-430 https://doi.org/10.1146/annurev.pa.09.040169.002223
  4. Brekhman.I.I 1963. Eleutherococcus senticosus a new medicinal herb of the araliaceae family. Second International Pharmachological Meeting. pp.97-102
  5. Elyakova LA, Kzizenko AK, Sova VV, Elyakov GB. 1996. (-)- Sessamin and (-)-savinin from Acanthopanax sessiliflorus and their Naur spectra. Khimiya Prirodnykh soedineii 2: 149-152
  6. Han YS. 2002. A Study on the effect of antiinflammatory plant extracts on melanogenesis. Ph.D. thesis. Ajou University. Suwon, Korea
  7. Hahn, DR., Kim CJ, and Kim JH . 1985. A study on chemical constituents of Acanthopanax koreanum nakai and its pharmacobiological activities. Yakhak Hoeji. 29: 357-361
  8. Heinemann T, Axtmann G, Von Bergmann K. 1993. Comparison of intestinal absorption of cholesterol with different plant sterols in man. Eur. J. Clin. Invest. 23: 827-831 https://doi.org/10.1111/j.1365-2362.1993.tb00737.x
  9. Hirata F, Fujita K, Ishikura Y, Hosoda K, Ishikawa H. 1996. Hypocholesterolemic effect of sesame lignan in humans. Atherosclerosis 122: 135-136 https://doi.org/10.1016/0021-9150(95)05769-2
  10. Jin SK. 1995. Effects of mugwort and coffee mixture on the growth and alcohol fermentation of Saccharomyces cerevisiae, Ph.D. thesis, Konkuk University, Seoul, Korea
  11. Jung KW, 1981. Studies on pharmacological activity of root bark of Acanthopanax chiisanensis Nakai. Bull. Kyung Hee. Pharma. Sci. 9: 21-29
  12. Kim MK, Jin YS, Heo SI, Shim TH, Sa JH, Wang MH. 2006. Studies for component analysis and antioxidant effect, antimicrobial activity in Acanthopanax senticosus harms. Kor. J. Phamacogn. 37: 151-156
  13. Korea Food & Drug Administration. 2008. Food Standards Codex. Korean Foods Industry Association, Seoul, Korea. pp. 599-637
  14. Lee SO, Park MY. 1980. Immobilization of Leuconostoc oenos cells for wine deacidification. Korean J. Food Sci. Technol. 12: 299-304
  15. Nishiyama N, Kahiko T, Iwai A, Saito H, Sanada S, Ida Y, Shoji J. 1985. Effect of eleutherococcus senticosus and its component on sex and learning behaviour and tyrosine hydroxylase activities of adrenal gloud and Hypothlamine in chronic stressed mice. Shoyagak zasshi 39: 238-242
  16. Shin WT, Lee KS .2004. The effect of Ogapi's ingestion exercise performance SOD, MDA for 12 weeks. Korean Sport Res. 15: 1309-1320
  17. Szolomecki S, Samochowiec I., Wojcicki J. Drozdzik M. 2000. The influence of active components of Eleutherococcus senticosus on cellular defence and physical fitness in man. Phytotherapy Res. 14: 30-35 https://doi.org/10.1002/(SICI)1099-1573(200002)14:1<30::AID-PTR543>3.0.CO;2-V
  18. Yook CS, Rho YS, Seo SH, Leem JY, Han DR. 1996. Chemical components of Acanthopanax divaricatus and anticancer effect in leaves. Yakhak Hoeji 40: 251-261
  19. Yoon JT, Seol IC, Kim SH, Kim HS. 2001. An experimental study of antiallergic activity of medicinal acupunture solution of Acanthopanax senticosus. J. Korean Acup. Moxi. Soc. 18: 136-149