Journal of Korean Society of Occupational and Environmental Hygiene (한국산업보건학회지)

Korean Industrial Hygiene Association (한국산업보건학회)
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A study on biomarker for biomonitoring of 1,3-butadiene inhalation exposure

1,3-부타디엔 호흡기 노출의 생체지표 대사물질에 대한 연구

  • Lee, Jin-Heon (Dept. of Environmental Education, Kongju National University)
  • 이진헌 (공주대학교 환경교육과)
  • Received : 2009.12.14
  • Accepted : 2010.03.23
  • Published : 2010.03.30
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Abstract

The purpose of this study is to investigate the appropriate metabolite as biomarker for the biomonitoring of 1,3-butadiene(BD) inhalation exposure. We measured the hemoglobin adducts which were extracted from the blood of the ICR mice inhalation exposure with 100ppm and 500ppm 1,3-butadiene for 2 weeks(5 hr/day ${\times}$ 5 days/week). Hemoglobin adducts were the (N-2-hydroxy-3 -butenyl) valine (HB Val) and (N-2,3,4-trihydroxy-butyl)valine (THB Val). Body weights of the exposure groups were significantly lower from 11 exposure post-day in 100ppm BD inhalation mice and from 7 exposure post-day in 500ppm BD inhalation mice than in control. The levels of HB Val are 0.8~1.7pmol/mg globin for 100ppm BD inhalation exposure, and 2.1~4.4 pmol/mg globin for 500ppm BD inhalation exposure. The levels of THB Val are 15.0~22.0 pmol/mg globin in 100ppm BD inhalation exposure, and 34.8~45.7 pmol/mg globin for 500ppm BD inhalation exposure. So the levels of THB Val and HB Val are proportional relationship with BD exposure level. THB Val is 12.9~18.8 times higher level that HB Val in 100ppm BD exposure group and 10.4~16.6 times higher level than HB Val in 500ppm BD exposure group. We concluded that THB Val is an appropriate metabolite as biomarker for the biomonitoring for BD inhalation exposure.

Keywords

References

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