Korean Journal of Radiology

  • 제11권6호
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  • Pages.673-682
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  • 2010
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  • 1229-6929(pISSN)
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  • 2005-8330(eISSN)
대한영상의학회 (The Korean Society of Radiology)
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Evaluation of Porcine Pancreatic Islets Transplanted in the Kidney Capsules of Diabetic Mice Using a Clinically Approved Superparamagnetic Iron Oxide (SPIO) and a 1.5T MR Scanner

  • Kim, Hoe-Suk (Department of Radiology, Seoul National University Hospital) ;
  • Kim, Hyoung-Su (Department of Radiology, Seoul National University Hospital) ;
  • Park, Kyong-Soo (Department of Internal Medicine, Seoul National University Hospital) ;
  • Moon, Woo-Kyung (Department of Radiology, Seoul National University Hospital)
  • 투고 : 2009.12.01
  • 심사 : 2010.07.22
  • 발행 : 2010.12.01
⟨ 이전 논문 다음 논문 ⟩

초록

Objective: To evaluate transplanted porcine pancreatic islets in the kidney capsules of diabetic mice using a clinically approved superparamagnetic iron oxide (SPIO) and a 1.5T MR scanner. Materials and Methods: Various numbers of porcine pancreatic islets labeled with Resovist, a carboxydextran-coated SPIO, were transplanted into the kidney capsules of normal mice and imaged with a 3D FIESTA sequence using a 1.5T clinical MR scanner. Labeled (n = 3) and unlabeled (n = 2) islets were transplanted into the kidney capsules of streptozotocin-induced diabetic mice. Blood glucose levels and MR signal intensities were monitored for 30 days post-transplantation. Results: There were no significant differences in viability or insulin secretion between labeled and unlabeled islets. A strong correlation ($r^2$ > 0.94) was evident between the number of transplanted islets and $T_2$ relaxation times quantified by MRI. Transplantation with labeled or unlabeled islets helped restore normal sustained glucose levels in diabetic mice, and nephrectomies induced the recurrence of diabetes. The MR signal intensity of labeled pancreatic islets decreased by 80% over 30 days. Conclusion: The transplantation of SPIO-labeled porcine islets into the kidney capsule of diabetic mice allows to restore normal glucose levels, and these islets can be visualized and quantified using a 1.5T clinical MR scanner.

키워드

과제정보

연구 과제 주관 기관 : NRF

참고문헌

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피인용 문헌

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  2. Magnetic separation of encapsulated islet cells labeled with superparamagnetic iron oxide nano particles vol.20, pp.4, 2013, https://doi.org/10.1111/xen.12042
  3. Imaging of transplanted islets by positron emission tomography, magnetic resonance imaging, and ultrasonography vol.5, pp.5, 2013, https://doi.org/10.4161/isl.26980
  4. Insulin-producing cells from embryonic stem cells rescues hyperglycemia via intra-spleen migration vol.4, pp.None, 2010, https://doi.org/10.1038/srep07586
  5. Transplantation of human mobilized mononuclear cells improved diabetic neuropathy vol.239, pp.3, 2010, https://doi.org/10.1530/joe-18-0516
  6. Transplantation of human mobilized mononuclear cells improved diabetic neuropathy vol.239, pp.3, 2010, https://doi.org/10.1530/joe-18-0516