Annals of Laboratory Medicine

Korean Society for Laboratory Medicine (대한진단검사의학회)
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Usefulness of Frozen-thawed-deglycerolized Red Blood Cells as Quality Control Materials for Red Blood Cell Deformability Test

  • Kim, Yu-Kyung (Department of Clinical Pathology, Kyungpook National University School of Medicine) ;
  • Won, Dong-Il (Department of Clinical Pathology, Kyungpook National University School of Medicine) ;
  • Kim, Hyun-Ok (Department of Laboratory Medicine, Yonsei University College of Medicine) ;
  • Shin, Se-Hyun (Department of Mechanical Engineering, Korea University School of Engineering) ;
  • Suh, Jang-Soo (Department of Clinical Pathology, Kyungpook National University School of Medicine)
  • Received : 2010.04.29
  • Accepted : 2010.11.02
  • Published : 2010.12.01
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Abstract

Background : The red blood cell (RBC) deformability test is a useful method for measuring the ability of RBCs to adapt their shape to the flow conditions. Using this test, several investigators have shown the relationship between RBC deformability and numerous clinical conditions. For the quality control (QC) of RBC deformability test, we evaluated whether frozen-thawed-deglycerolized RBCs can be used as QC materials. Methods : Packed RBCs were frozen with 40% (wt/vol) glycerol and stored at $-80^{\circ}C$ for 3 months. For 10 different frozen RBC panels, RBCs were thawed, deglycerolized and stored at $4^{\circ}C$ for 4 weeks. Using microfluidic ektacytometer, we measured RBC deformability of the thawed RBCs. The stability of thawed RBCs was tested once a day for 28 days of storage time and was analyzed by simple regression analysis. The precision of the test using thawed RBCs was analyzed for 7 days of storage time by calculation of CV values of intra-assay (10 measurements/assay) and between-day measurements. Results : Frozen-thawed-deglycerolized RBCs were stable for 1 week. Within-run and between-day precisions of the RBC deformability test during 7 days of storage of thawed RBCs were 1.4-2.9%, and 1.9-2.8%, respectively. Conclusions : Frozen-thawed-deglycerolized RBCs used in RBC deformability test showed satisfactory within-run and between-run precisions and stability for one week after thawing, and may be used as QC materials for this test.

배경 : 적혈구 변형능 검사는 유체흐름에서의 적혈구의 형태변형 정도를 측정하는 유용한 방법이다. 다양한 연구자들이 이 검사를 이용하여 적혈구 변형능과 여러 임상 질환과의 관련성을 밝히고 있다. 적혈구 변형능 검사의 질 관리 측면에서, 저자들은 동결해동적혈구의 정도관리물질로서의 유용성을 평가해 보았다. 방법 : 적혈구 농축액에 40% 글리세롤을 첨가하여 냉동시킨 후 $-80^{\circ}C$에서 3개월간 보관하였다. 10개의 냉동적혈구 표준품을 대상으로 해동과 글리세롤 제거 후 $4^{\circ}C$에서 4주간 보관하며, microfluidic ektacytometer를 이용하여 적혈구 변형능을 측정하였다. 해동적혈구의 안정성 평가를 위해서 1일 1회씩 28일간 측정한 후 단순회귀분석을 실시하였다. 해동 후 7일간 검사 내 정밀도(10회 중복 측정) 및 검사일간 정밀도는 변이계수로 평가하였다. 결과 : 동결해동적혈구는 일주일간 안정한 것으로 나타났다. 해동 후 7일 동안 적혈구 변형능 측정 시 검사 내 정밀도는 1.4-2.9%였고, 검사일간 정밀도는 1.9-2.8%로 나타났다. 결론 : 동결해동적혈구는 해동 후 일주일간 안정적이면서, 검사 내 및 검사일간 정밀도가 모두 우수했으므로 적혈구 변형 능 검사의 안정적인 정도관리물질로 이용할 수 있을 것으로 사료된다.

Keywords

Acknowledgement

Supported by : Kyungpook National University

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