Anatomy and Cell Biology

Korean Association of Anatomists (대한해부학회)
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Ethanol down regulates the expression of myelin proteolipid protein in the rat hippocampus

  • Lee, Dong-Hoon (Department of Anatomy & Neurobiology, Institute of Health Sciences, Medical Research Center for Neural Dysfunction, Gyeongsang National University School of Medicine) ;
  • Jeong, Jin-Young (Department of Anatomy & Neurobiology, Institute of Health Sciences, Medical Research Center for Neural Dysfunction, Gyeongsang National University School of Medicine) ;
  • Kim, Yoon-Sook (Department of Anatomy & Neurobiology, Institute of Health Sciences, Medical Research Center for Neural Dysfunction, Gyeongsang National University School of Medicine) ;
  • Kim, Joon-Soo (Department of Neurosurgery, Samsung Changwon Hospital, Sungkyunkwan University School of Medicine) ;
  • Cho, Yong-Woon (Department of Neurosurgery, Samsung Changwon Hospital, Sungkyunkwan University School of Medicine) ;
  • Roh, Gu-Seob (Department of Anatomy & Neurobiology, Institute of Health Sciences, Medical Research Center for Neural Dysfunction, Gyeongsang National University School of Medicine) ;
  • Kim, Hyun-Joon (Department of Anatomy & Neurobiology, Institute of Health Sciences, Medical Research Center for Neural Dysfunction, Gyeongsang National University School of Medicine) ;
  • Kang, Sang-Soo (Department of Anatomy & Neurobiology, Institute of Health Sciences, Medical Research Center for Neural Dysfunction, Gyeongsang National University School of Medicine) ;
  • Cho, Gyeong-Jae (Department of Anatomy & Neurobiology, Institute of Health Sciences, Medical Research Center for Neural Dysfunction, Gyeongsang National University School of Medicine) ;
  • Choi, Wan-Sung (Department of Anatomy & Neurobiology, Institute of Health Sciences, Medical Research Center for Neural Dysfunction, Gyeongsang National University School of Medicine)
  • Received : 2010.07.28
  • Accepted : 2010.09.06
  • Published : 2010.09.30
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Abstract

It is well known that chronic ethanol treatment affects the synthesis of RNA and protein in the brain and the maintenance and function of nervous system. The changes in myelination-related genes are most prominent in human alcoholics. Previously, our cDNA microarray study showed altered Proteolipid protein (PLP), a major protein of central myelin. The present study aimed to gain more understanding of the expression of PLP after chronic ethanol treatment. Male Sprague-Dawley rats were daily treated with ethanol (15% in saline, 3 g/kg, i.p.) or saline for 14 days. Messenger RNAs from hippocampus of each group were subjected to cDNA expression array hybridization to determine the differential gene expressions. Among many ethanol responsive genes, PLP was negatively regulated by ethanol treatment, which is one of the most abundant proteins in the CNS and has an important role in the stabilization of myelin sheath. Using northern blot and immunohistochemical analysis, we showed the change in expression level of PLP mRNA and protein after ethanol treatment. PLP mRNA and protein were decreased in hippocampus of rat with chronic ethanol exposure, suggesting that ethanol may affect the stabilization of myelin sheath through the modulation of PLP expression and induce the pathophysiology of alcoholic brain.

Keywords

Acknowledgement

Supported by : National Research Foundation of Korea (NRF)

References

  1. Boison D, Bussow H, D'Urso D, Muller HW, Stoffel W. (1995). Adhesive properties of proteolipid protein are responsible for the compaction of CNS myelin sheaths. J Neurosci 15: 5502-5513
  2. Boison D, Stoffel W. (1994). Disruption of the compacted myelin sheath of axons of the central nervous system in proteolipid protein-deficient mice. Proc Natl Acad Sci U S A 91: 11709-11713 https://doi.org/10.1073/pnas.91.24.11709
  3. Bowers BJ, Radcliffe RA, Smith AM, Miyamoto-Ditmon J, Wehner JM. (2006). Microarray analysis identifies cerebellar genes sensitive to chronic ethanol treatment in PKCgamma mice. Alcohol 40: 19-33 https://doi.org/10.1016/j.alcohol.2006.09.004
  4. Daigo M, Arai Y, Oshida K, et al. (2008). Effect of hypoxic-ischemic injury on serine palmitoyltransferase activity in the developing rat brain. Pathobiology 75: 330-334 https://doi.org/10.1159/000164217
  5. Deitrich RA, Dunwiddie TV, Harris RA, Erwin VG. (1989). Mechanism of action of ethanol: initial central nervous system actions. Pharmacol Rev 41: 489-537
  6. Duggan DJ, Bittner M, Chen Y, Meltzer P, Trent JM. (1999). Expression profiling using cDNA microarrays. Nat Genet 21(1 Suppl): 10-14 https://doi.org/10.1038/4434
  7. Duncan ID, Hammang JP, Trapp BD. (1987). Abnormal compact myelin in the myelin-deficient rat: absence of proteolipid protein correlates with a defect in the intraperiod line. Proc Natl Acad Sci U S A 84: 6287-6291 https://doi.org/10.1073/pnas.84.17.6287
  8. Griffiths I, Klugmann M, Anderson T, et al. (1998). Axonal swellings and degeneration in mice lacking the major proteolipid of myelin. Science 280: 1610-1613 https://doi.org/10.1126/science.280.5369.1610
  9. Inoue Y, Sugihara Y, Nakagawa S, Shimai K. (1973). The morphological changes of oligodendroglia during the formation of myelin sheaths--Golgi study and electron microscopy. Okajimas Folia Anat Jpn 50: 327-343 https://doi.org/10.2535/ofaj1936.50.5_327
  10. Klugmann M, Schwab MH, Puhlhofer A, et al. (1997). Assembly of CNS myelin in the absence of proteolipid protein. Neuron 18: 59-70 https://doi.org/10.1016/S0896-6273(01)80046-5
  11. Kril JJ, Halliday GM. (1999). Brain shrinkage in alcoholics: a decade on and what have we learned? Prog Neurobiol 58: 381-387 https://doi.org/10.1016/S0301-0082(98)00091-4
  12. Lewohl JM, Dodd PR, Mayfield RD, Harris RA. (2001). Application of DNA microarrays to study human alcoholism. J Biomed Sci 8: 28-36 https://doi.org/10.1007/BF02255968
  13. Liu J, Lewohl JM, Dodd PR, Randall PK, Harris RA, Mayfield RD. (2004). Gene expression profiling of individual cases reveals consistent transcriptional changes in alcoholic human brain. J Neurochem 90: 1050-1058 https://doi.org/10.1111/j.1471-4159.2004.02570.x
  14. Mobius W, Patzig J, Nave KA, Werner HB. (2008). Phylogeny of proteolipid proteins: divergence, constraints, and the evolution of novel functions in myelination and neuroprotection. Neuron Glia Biol 4: 111-127 https://doi.org/10.1017/S1740925X0900009X
  15. Ollat H, Sebban C. (1983). Histological lesions and changes in neurotransmitter systems in the aged brain. Discussion of their pathogenic role. Presse Med 12: 809-814
  16. Peters A, Josephson K, Vincent SL. (1991). Effects of aging on the neuroglial cells and pericytes within area 17 of the rhesus monkey cerebral cortex. Anat Rec 229: 384-398 https://doi.org/10.1002/ar.1092290311
  17. Rosenbluth J, Nave KA, Mierzwa A, Schiff R. (2006). Subtle myelin defects in PLP-null mice. Glia 54: 172-182 https://doi.org/10.1002/glia.20370
  18. Rosenbluth J, Stoffel W, Schiff R. (1996). Myelin structure in proteolipid protein (PLP)-null mouse spinal cord. J Comp Neurol 371: 336-344 https://doi.org/10.1002/(SICI)1096-9861(19960722)371:2<336::AID-CNE12>3.0.CO;2-Q
  19. Ryabinin AE. (1998). Role of hippocampus in alcohol-induced memory impairment: implications from behavioral and immediate early gene studies. Psychopharmacology (Berl) 139: 34-43 https://doi.org/10.1007/s002130050687
  20. Saito M, Smiley J, Toth R, Vadasz C. (2002). Microarray analysis of gene expression in rat hippocampus after chronic ethanol treatment. Neurochem Res 27: 1221-1229 https://doi.org/10.1023/A:1020937728506
  21. Samson HH, Harris RA. (1992). Neurobiology of alcohol abuse. Trends Pharmacol Sci 13: 206-211 https://doi.org/10.1016/0165-6147(92)90065-E
  22. Sarret C, Combes P, Micheau P, Gelot A, Boespflug-Tanguy O, Vaurs-Barriere C. (2010). Novel neuronal proteolipid protein isoforms encoded by the human myelin proteolipid protein 1 gene. Neuroscience 166: 522-538 https://doi.org/10.1016/j.neuroscience.2009.12.047
  23. Tanaka H, Ma J, Tanaka KF, et al. (2009). Mice with altered myelin proteolipid protein gene expression display cognitive deficits accompanied by abnormal neuron-glia interactions and decreased conduction velocities. J Neurosci 29: 8363-8371 https://doi.org/10.1523/JNEUROSCI.3216-08.2009
  24. Walker DW, Barnes DE, Zornetzer SF, Hunter BE, Kubanis P. (1980). Neuronal loss in hippocampus induced by prolonged ethanol consumption in rats. Science 209: 711-713 https://doi.org/10.1126/science.7394532

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