Anatomy and Cell Biology

Korean Association of Anatomists (대한해부학회)
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Systemic injection of recombinant human erythropoietin after focal cerebral ischemia enhances oligodendroglial and endothelial progenitor cells in rat brain

  • Kim, Young-Jae (Department of Laboratory Medicine, Masansamsung Medical Center, School of Medicine, Sungkyunkwan University) ;
  • Jung, Yong-Wook (Department of Anatomy, College of Medicine, Dongguk University)
  • Received : 2010.02.05
  • Accepted : 2010.05.03
  • Published : 2010.06.30
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Abstract

Erythropoietin (EPO) has been demonstrated the ability of recombinant human erythropoietin (r-Hu-EPO), when administered intracerebro-ventricularly, to improve stroke outcome through the reduction of stroke damage. In a brain ischemic model, however, systemic administration of r-Hu-EPO has not been intensely investigated given that in general, large glycosylated molecules have been deemed incapable of crossing the blood-brain barrier. In this study, administration of r-Hu-EPO for 4 days, intraperitoneally aft er ischemia-reperfusion (I-R) increased the number of bromodeoxyuridine (BrdU)-positive cells in the penumbra ($10.1{\pm}1.4$, n=5, P<0.05) and in the subventricular zone (SVZ) of the lateral ventricle (LV) ($25{\pm}2.7$, n=5, P<0.05) as compared with those of I-R (penumbra: $2.5{\pm}0.7$; SVZ of LV: $3.8{\pm}1.5$). A significant increase of BrdU-positive cells in these areas was coincident with a strong immunoreactivity of oligodendrocyte progenitor cell marker (2', 3'-cyclic nucleotide 3'-phosphodiesterase). Furthermore, r-Hu-EPO administration increased the number of BrdU-positive cells in the choroid plexus ($7.8{\pm}2.3$, n=5, P<0.05) and in cerebral blood vessels ($3.5{\pm}1.3$, n=5, P<0.05) when compared with those of I-R (choroid plexus: $1.2{\pm}0.5$; cerebral blood vessels: $0.6{\pm}0.1$). These results suggest that, even when systemically administered, r-Hu-EPO may have therapeutic potential for stroke via the proliferation of oligodendroglial and endothelial progenitor cells.

Keywords

Acknowledgement

Supported by : Dongguk Research Fund, Dongguk University

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