Anatomy and Cell Biology

  • 제43권1호
  • /
  • Pages.72-77
  • /
  • 2010
  • /
  • 2093-3665(pISSN)
  • /
  • 2093-3673(eISSN)
대한해부학회 (Korean Association of Anatomists)
권호 표지
DOI QR코드

DOI QR Code

TBR2-immunopsitive unipolar brush cells are associated with ectopic zebrin II-immunoreactive Purkinje cell clusters in the cerebellum of scrambler mice

  • Chung, Seung-Hyuk (Department of Cell Biology and Human Anatomy, School of Medicine, University of California) ;
  • Kim, Chul-Tae (Department of Anatomy, College of Medicine, Konyang University) ;
  • Jeong, Young-Gil (Department of Anatomy, College of Medicine, Konyang University) ;
  • Lee, Nam-Seob (Department of Anatomy, College of Medicine, Konyang University)
  • 투고 : 2010.01.26
  • 심사 : 2010.03.03
  • 발행 : 2010.03.30
⟨ 이전 논문 다음 논문 ⟩

초록

Unipolar brush cells (UBCs) are excitatory interneurons with their somata located in the granular layer. Recently, T-brain factor 2 (Tbr2) was shown to be expressed in a subset of UBCs in mouse cerebellum. Scrambler mice exhibit severe cerebellum abnormalities, including the failure of embryonic Purkinje cell dispersal and a complete absence of foliation due to a mutation in the disabled-1 adaptor protein. Since most UBC markers are expressed postnatally, it has proven diffi cult to identify the relationship between developing Purkinje cell clusters and migrating UBCs. Because scrambler mice closely mimic normal embryonic day 18 cerebellum, we examined whether Tbr2-positive UBCs are associated with Purkinje cell cluster markers such as zebrin II, which is the most studied compartmentation marker in the cerebellum. We investigated the distribution of Tbr2-positive UBCs in this mutant by using anti-Tbr2 immunocytochemistry. Th e data revealed that Tbr2 immunoreactivity was exclusively present in the nucleus of UBCs in scrambler cerebellum. Based on expression data, a Tbr2-positive UBC map was constructed. In addition, Tbr2-positive UBCs are found associated with ectopic zebrin II-immunoreactive Purkinje cell clusters in scrambler cerebellum. Th ese data suggest that UBCs use Purkinje cell compartmentation to migrate into their final position through interactions with the embryonic array of specific Purkinje cell subtypes.

키워드

과제정보

연구 과제 주관 기관 : Konyang University Myunggok Research Fund

참고문헌

  1. Abbott LC, Jacobowitz DM. (1995). Development of calretinin-immunoreactive unipolar brush-like cells and an afferent pathway to the embryonic and early postnatal mouse cerebellum. Anat Embryol Berl 191: 541-559
  2. Ahn AH, Dziennis S, Hawkes R, Herrup K. (1994). The cloning of zebrin II reveals its identity with aldolase C. Development 120: 2081-2090
  3. Altman J, Bayer SA. (1977). Time of origin and distribution of a new cell type in the rat cerebellar cortex. Exp Brain Res 29: 265-274
  4. Armstrong CL, Hawkes R. (2000). Pattern formation in the cerebellar cortex. Biochem Cell Biol 78: 551-562 https://doi.org/10.1139/o00-071
  5. Brochu G, Maler L, Hawkes R. (1990). Zebrin II: a polypeptide antigen expressed selectively by Purkinje cells reveals compartments in rat and fi sh cerebellum. J Comp Neurol 291: 538-552, 199. https://doi.org/10.1002/cne.902910405
  6. Chung SH, Sillitoe RV, Croci L, Badaloni A, Consalez G, Hawkes R. (2009). Purkinje cell phenotype restricts the distribution of unipolar brush cells. Neuroscience 164: 1496-1508 https://doi.org/10.1016/j.neuroscience.2009.09.080
  7. Eisenman LM. (2000). Antero-posterior boundaries and compartments in the cerebellum: evidence from selected neurological mutants. Prog Brain Res 124: 23-30 https://doi.org/10.1016/S0079-6123(00)24005-3
  8. Englund C, Kowalczyk T, Daza RA, et al. (2006). Unipolar brush cells of the cerebellum are produced in the rhombic lip and migrate through developing white matter. J Neurosci 26: 9184-9195 https://doi.org/10.1523/JNEUROSCI.1610-06.2006
  9. Floris A, Dino M, Jacobowitz DM, et al. (1994). Th e unipolar brush cells of the rat cerebellar cortex and cochlear nucleus are calretinin-positive: a study by light and electron microscopic immunocytochemistry. Anat Embryol (Berl) 189: 495-520
  10. Goldowitz D, Cushing RC, Laywell E, et al. (1997). Cerebellar disorganization characteristic of reeler in scrambler mutant mice despite presence of reelin. J Neurosci 17: 8767-8777
  11. Hawkes R. (1997). An anatomical model of cerebellar modules. Prog Brain Res 114: 39-52 https://doi.org/10.1016/S0079-6123(08)63357-9
  12. Hawkes R, Eisenman LM. (1997). Stripes and zones: the origins of regionalization of the adult cerebellum. Perspect Dev Neurobiol 5: 95-105
  13. Hawkes R, Gravel C. (1991). Th e modular cerebellum. Prog Neurobiol 36: 309-327 https://doi.org/10.1016/0301-0082(91)90004-K
  14. Howell BW, Hawkes R, Soriano P, Cooper JA. (1997). Neuronal position in the developing brain is regulated by mouse disabled-1. Nature 389: 733-737 https://doi.org/10.1038/39607
  15. Mugnaini E, Floris A. (1994). The unipolar brush cell: a neglected neuron of the mammalian cerebellar cortex. J Comp Neurol 339: 174-180 https://doi.org/10.1002/cne.903390203
  16. Nunzi MG, Birnstiel S, Bhattacharyya BJ, Slater NT, Mugnaini E. (2001). Unipolar brush cells form a glutamatergic projection system within the mouse cerebellar cortex. J Comp Neurol 434: 329-341 https://doi.org/10.1002/cne.1180
  17. Nunzi MG, Mugnaini E. (2000). Unipolar brush cell axons form a large system of intrinsic mossy fibers in the postnatal vestibulocerebellum. J Comp Neurol 422: 55-65 https://doi.org/10.1002/(SICI)1096-9861(20000619)422:1<55::AID-CNE4>3.0.CO;2-9
  18. Nunzi MG, Shigemoto R, Mugnaini E. (2002). Differential expression of calretinin and metabotropic glutamate receptor mGluR1 alpha defi nes subsets of unipolar brush cells in mouse cerebellum. J Comp Neurol 451: 189-199 https://doi.org/10.1002/cne.10344
  19. Ozol KO, Hawkes R. (1997). compartmentation of the granular layer of the cerebellum. Histol Histopathol 12: 171-184
  20. Ozol K, Hayden JM, Oberdick J, Hawkes R. (1999). Transverse zones in the vermis of the mouse cerebellum. J Comp Neurol 412: 95-111 https://doi.org/10.1002/(SICI)1096-9861(19990913)412:1<95::AID-CNE7>3.0.CO;2-Y
  21. Sheldon M, Rice DS, D'Arcangelo G, et al. (1997). Scrambler and yotari disrupt the disabled gene and produce a reelerlike phenotype in mice. Nature 389: 730-733 https://doi.org/10.1038/39601
  22. Sotelo C, Chedotal A. (2005). Development of the olivocerebellar system: migration and formation of cerebellar maps. Prog Brain Res 148: 1-20 https://doi.org/10.1016/S0079-6123(04)48001-7
  23. Sweet HO, Bronson RT, Johnson KR, Cook SA, Davisson MT. (1996). Scrambler, a new neurological mutation of the mouse with abnormalities of neuronal migration. Mamm Genome 7: 798-802 https://doi.org/10.1007/s003359900240

피인용 문헌

  1. Intrinsic and Synaptic Properties Shaping Diverse Behaviors of Neural Dynamics vol.14, pp.None, 2010, https://doi.org/10.3389/fncom.2020.00026