Anatomy and Cell Biology

Korean Association of Anatomists (대한해부학회)
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TBR2-immunopsitive unipolar brush cells are associated with ectopic zebrin II-immunoreactive Purkinje cell clusters in the cerebellum of scrambler mice

  • Chung, Seung-Hyuk (Department of Cell Biology and Human Anatomy, School of Medicine, University of California) ;
  • Kim, Chul-Tae (Department of Anatomy, College of Medicine, Konyang University) ;
  • Jeong, Young-Gil (Department of Anatomy, College of Medicine, Konyang University) ;
  • Lee, Nam-Seob (Department of Anatomy, College of Medicine, Konyang University)
  • Received : 2010.01.26
  • Accepted : 2010.03.03
  • Published : 2010.03.30
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Abstract

Unipolar brush cells (UBCs) are excitatory interneurons with their somata located in the granular layer. Recently, T-brain factor 2 (Tbr2) was shown to be expressed in a subset of UBCs in mouse cerebellum. Scrambler mice exhibit severe cerebellum abnormalities, including the failure of embryonic Purkinje cell dispersal and a complete absence of foliation due to a mutation in the disabled-1 adaptor protein. Since most UBC markers are expressed postnatally, it has proven diffi cult to identify the relationship between developing Purkinje cell clusters and migrating UBCs. Because scrambler mice closely mimic normal embryonic day 18 cerebellum, we examined whether Tbr2-positive UBCs are associated with Purkinje cell cluster markers such as zebrin II, which is the most studied compartmentation marker in the cerebellum. We investigated the distribution of Tbr2-positive UBCs in this mutant by using anti-Tbr2 immunocytochemistry. Th e data revealed that Tbr2 immunoreactivity was exclusively present in the nucleus of UBCs in scrambler cerebellum. Based on expression data, a Tbr2-positive UBC map was constructed. In addition, Tbr2-positive UBCs are found associated with ectopic zebrin II-immunoreactive Purkinje cell clusters in scrambler cerebellum. Th ese data suggest that UBCs use Purkinje cell compartmentation to migrate into their final position through interactions with the embryonic array of specific Purkinje cell subtypes.

Keywords

Acknowledgement

Supported by : Konyang University Myunggok Research Fund

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