Bulletin of the Korean Chemical Society

Korean Chemical Society (대한화학회)
권호 표지
DOI QR코드

DOI QR Code

Quantitative and Pattern Recognition Analyses for the Quality Evaluation of Magnoliae Flos by HPLC

  • Fang, Zhe (College of Pharmacy, Catholic University of Daegu) ;
  • Shen, Chang Min (College of Pharmacy, Catholic University of Daegu) ;
  • Moon, Dong-Cheul (College of Pharmacy, Chungbuk National University) ;
  • Son, Kun-Ho (College of Life Science, Andong National University) ;
  • Son, Jong-Keun (College of Pharmacy, Yeungnam University) ;
  • Woo, Mi-Hee (College of Pharmacy, Catholic University of Daegu)
  • Received : 2010.03.10
  • Accepted : 2010.09.15
  • Published : 2010.11.20
Download PDF
⟨ Previous Next ⟩

Abstract

In this study, quantitative and pattern recognition analysis for the quality evaluation of Magnoliae Flos using HPLC/UV was developed. For quantitative analysis, eleven major bioactive lignan compounds were determined. The separation conditions employed for HPLC/UV were optimized using ODS $C_{18}$ column ($250{\times}4.6\;mm$, $5\;{\mu}m$) with isocratic elution of acetonitrile and water with 1% acetic acid as the mobile phase at a flow rate of 1.0 mL/min and a detection wavelength of 278 nm. These methods were fully validated with respect to the linearity, accuracy, precision, recovery, and robustness. The HPLC/UV method was applied successfully to the quantification of eleven major compounds in the extract of Magnoliae Flos. The HPLC analytical method for pattern recognition analysis was validated by repeated analysis of twenty one reference samples corresponding to seven different species of Magnoliae Flos and nine samples purchased from market. The results indicate that the established HPLC/UV method is suitable for the quantitative analysis and quality control of multi-components in Magnoliae Flos.

Keywords

References

  1. Islam, M. N.; Lee, S. K.; Jeong, S. Y.; Kim, D. H.; Jin, C. B.; Yoo, H. H. Bull. Korean Chem. Soc. 2009, 30, 137. https://doi.org/10.5012/bkcs.2009.30.1.137
  2. Kimura, I.; Kimura, M.; Yoshizaki, M.; Yanada, K.; Kadata, S.; Kikuchi, T. Planta Med. 1983, 48, 43. https://doi.org/10.1055/s-2007-969876
  3. Watanabe, H.; Shimizu, H. Japan. J. Pharmacol. 1989, 50, 75. https://doi.org/10.1254/jjp.50.75
  4. Kimura, M.; Suzuki, J.; Yamada, T.; Yoshizaki, M.; Kikuchi, T.; Kadota, S.; Matsuda, S. Planta Med. 1985, 50, 291.
  5. Wang, W. W.; Shen, Y. J.; Qi, Y. Shanxi Yiyao Zazhi 2000, 29, 206.
  6. Wang, W. W.; Shen, Y. J.; Qi, Y.; Liu, P. P. Zhonggo Shouyi Xuebao 2005, 25, 301.
  7. Lim, H. B.; Sohn, H. O.; Lee, D. W. Korean J. Medicinal Crop Sci. 2005, 13, 245.
  8. Tsuruga, T.; Ebizuka, Y.; Nakajima, J.; Chun, Y. T.; Noguchi, H.; Iitaka, Y.; Sankawa, U. Chem. Pharm. Bull. 1991, 39, 3265. https://doi.org/10.1248/cpb.39.3265
  9. Kobayash, S.; Kimura, I.; Kimura, M. Biol. Pharm. Bull. 1996, 19, 1304. https://doi.org/10.1248/bpb.19.1304
  10. Pan, J. X.; Hensens, O.; Zink, D.; Chang, M. N.; Hwang, S. B. Phytochemistry 1987, 26, 1377. https://doi.org/10.1016/S0031-9422(00)81816-6
  11. Yin, M. H.; Kang, D. G.; Choi, D. H.; Kwon, T. O.; Lee, H. S. J. Ethnopharmacol. 2005, 99, 113. https://doi.org/10.1016/j.jep.2005.02.013
  12. Yu, Z. Y.; Sun, Z. F.; Su, B. Z.; Liu, Q. Zhongcaoyao 2004, 35, 574.
  13. Fang, H.; Guo, Q.; Su, W.; Deng, F.; Wang, K. Q. Yaowu Fenxi Zazhi 2002, 22, 342.
  14. Xu, L. H.; Cui, B. G.; Yu, Z. Y. Yaowu Fenxi Zazhi 2003, 23, 426.
  15. Choi, J. Y.; Choi, E. H.; Oh, J. S.; Jung, H. W.; Kim, D. C.; Lee, H. S.; Jong, G.; Kim, J. A.; Son, J. K.; Lee, S. H. Saengyak Hakhoechi 2007, 38, 281.
  16. Chen, G. F.; Feng, Y. F.; Meng, Q. Guangdong Yaoxueyuan Xuebao 2003, 19, 99.
  17. Ma, Y. L.; Han, G. Q. Zhongguo Zhongyao Zazhi 1995, 20, 102.
  18. Kuroyanagi, M.; Yoshida, K.; Yamamoto, A.; Miwa, M. Chem. Pharm. Bull. 2000, 48, 832. https://doi.org/10.1248/cpb.48.832
  19. Miyauchi, T.; Ozawa, S. Phytochemistry 1998, 47, 665. https://doi.org/10.1016/S0031-9422(97)00458-5
  20. Miyazawa, M.; Kasahara, H.; Kameoka, H. Phytochemistry 1995, 39, 1027. https://doi.org/10.1016/0031-9422(95)00168-7
  21. Fish, F.; Waterman, P. G. Phytochemistry 1972, 11, 1527. https://doi.org/10.1016/S0031-9422(00)90138-9
  22. Pelter, A.; Ward, R. S.; Nishono, C. Tetrahedron Lett. 1977, 18, 4137. https://doi.org/10.1016/S0040-4039(01)83447-X
  23. Lida, T.; Nakano, M.; Ito, K. Phytochemistry 1983, 22, 211. https://doi.org/10.1016/S0031-9422(00)80091-6
  24. Yoshida, S.; Yamanaka, T.; Miyake, T.; Moritani, Y.; Ohmizu, H.; Iwasaki, T. Tetrahedron 1997, 53, 9585. https://doi.org/10.1016/S0040-4020(97)00643-1
  25. Lima, O. A.; Gottlieb, O. R.; Magalhaes, M. T. Phytochemistry 1972, 11, 2031. https://doi.org/10.1016/S0031-9422(00)90169-9
  26. Li, J.; Tanaka, M.; Kurasawa, K.; Ikeda, T.; Nohara, T. Chem. Pharm. Bull. 2005, 53, 235. https://doi.org/10.1248/cpb.53.235

Cited by

  1. Quantitative and Pattern Recognition Analyses of Five Marker Compounds in Raphani Semen using High-Performance Liquid Chromatography vol.36, pp.9, 2015, https://doi.org/10.1002/bkcs.10450
  2. Paenibacillus kyungheensis sp. nov., isolated from flowers of magnolia vol.65, pp.11, 2015, https://doi.org/10.1099/ijsem.0.000521
  3. Analysis of lignans in Magnoliae Flos by turbulent flow chromatography with online solid-phase extraction and high-performance liquid chromatography with tandem mass spectrometry vol.39, pp.7, 2016, https://doi.org/10.1002/jssc.201501167
  4. HPLC를 이용한 낙지다리 추출물의 (-)-­Epicatechin gallate 분석법 개발 vol.45, pp.1, 2010, https://doi.org/10.15230/scsk.2019.45.1.87
  5. HPLC를 이용한 노루오줌 추출물의 Avicularin 및 Quercitrin 분석법 개발 vol.45, pp.4, 2010, https://doi.org/10.15230/scsk.2019.45.4.333