Korean Journal of Veterinary Service (한국동물위생학회지)

The Korean Society of Veterinary Service (한국동물위생학회)
권호 표지

Investigation of infection rate and genetic sequence analysis of chicken infectious anemia virus

닭 전염성빈혈 감염률 및 유전자 분석

  • Chu, Keum-Suk (Jeongeup-Branch, Jeonbuk Institute of Livestock & Veterinary Research) ;
  • Kang, Mi-Seon (Jeongeup-Branch, Jeonbuk Institute of Livestock & Veterinary Research) ;
  • Song, Hee-Jong (College of Veterinary Medicine and Korea Zoonoses Research Institute, Chonbuk National University) ;
  • Lee, Jeong-Won (Jeongeup-Branch, Jeonbuk Institute of Livestock & Veterinary Research)
  • 추금숙 (전라북도축산위생연구소 정읍지소) ;
  • 강미선 (전라북도축산위생연구소 정읍지소) ;
  • 송희종 (전북대학교 수의과대학) ;
  • 이정원 (전라북도축산위생연구소 정읍지소)
  • Received : 2010.02.10
  • Accepted : 2010.03.21
  • Published : 2010.03.30
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Abstract

Chicken anemia virus (CAV) has been recognized as an immunosuppressive agent and plays role as an etiological agent of multifactorial diseases in chicken. In this study, we investigated distribution of CAV antibody by ELISA and the virus gene by PCR in poultry farms in Jeongeup, Jeonbuk province. In the test using ELISA kit, 41 (95.3%) of 43 flocks and 88.6% of the individual chickens were positive, respectively. By PCR, 90.9% of the broiler breeders and 75.0% of White-semi breeders were found positive, respectively. All hatchery was negative by PCR. Of the clinical cases from 49 poultry flocks, 87.5% of flocks and 54.7% for each samples were found positive by ELISA, respectively. By PCR test, 21 (42.9%) of 49 flocks were positive. Major clinical signs of the infected flocks were growth retardation, femoral subcutaneous bleeding, depression, limping, and continuing selection. The genetic analysis of separate N genes of CAV showed highly homologous each other. The nucleotide sequence of field isolates had homology ranged from 99.9% to 97.5% with Chinese strains, and 99.9% to 99.6% with Japanese strain. Phylogenetic analysis based on the N gene of CAV isolates showed the closely relation with Chinese strains. The results of this survey could be used as basic data for development of vaccine.

Keywords

References

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