Journal of Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Anti-obesity Effects of Tae-Um-Jo-Wee-Tang and Do-Dam-Tang in Female Rats with Diet-induced Obesity

고지방식이로 비만을 유도한 암컷 백서에서 태음조위탕과 도담탕의 항비만 효과 및 기전

  • Park, Sun-Min (Department of Food and Nutrition, College of Natural Science, Basic Science Institutes, Hoseo University) ;
  • Ahn, Il-Sung (Department of Food and Nutrition, College of Natural Science, Basic Science Institutes, Hoseo University) ;
  • Kim, Da-Sol (Department of Food and Nutrition, College of Natural Science, Basic Science Institutes, Hoseo University) ;
  • Kang, Sun-A (Department of Food and Nutrition, College of Natural Science, Basic Science Institutes, Hoseo University) ;
  • Kwon, Dae-Young (Emerging Innovative Technology Research Division, Korean Food Research Institutes) ;
  • Yang, Hye-Jeong (Emerging Innovative Technology Research Division, Korean Food Research Institutes)
  • 박선민 (호서대학교 자연과학대학 기초과학연구소 식품영양학과) ;
  • 안일성 (호서대학교 자연과학대학 기초과학연구소 식품영양학과) ;
  • 김다솔 (호서대학교 자연과학대학 기초과학연구소 식품영양학과) ;
  • 강선아 (호서대학교 자연과학대학 기초과학연구소 식품영양학과) ;
  • 권대영 (한국식품연구원, 미래전략가술연구본부, 바이오제론연구단) ;
  • 양혜정 (한국식품연구원, 미래전략가술연구본부, 바이오제론연구단)
  • Received : 2009.12.28
  • Accepted : 2010.01.13
  • Published : 2010.03.31
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Abstract

Tae-Um-Jo-wee-Tang (TUJWT) and Do-Dam-Tang (DDT) have been used as an anti-obesity herbal medicine but their effect and mechanism of action have not been studied. We investigated the effects of TUJWT and DDT on energy and glucose homeostasis using Sprague Dawley female rats with diet-induced obesity. The mechanisms of action of TUJWT and DDT were studied whether they had anti-obesity effects. Rats fed a high-fat diet were divided into 3 groups: rats in each group received 2 g water extracts of modified TUJWT and DDT, or 2 g cellulose per kg body weight (a negative control) on a daily basis. A further group was fed a low-fat diet as a positive control. We found that DDT significantly decreased body weight and body fat (mesenteric fat and retroperitoneal fat) more than the control. This decrease was due to the reduction in energy intake but no changes of energy expenditure. However, DDT increased fat oxidation as a major energy source than the control. In addition, modified TUJWT and DDT improved glucose tolerance without changing serum insulin levels during an oral glucose tolerance test. In conclusion, DDT have a better anti-obesity effect than TUJWT by decreasing energy intake in female rats with diet-induced obesity. It also improves glucose tolerance.

본 연구에서는 고지방식이로 비만을 유도한 암컷 백서에게 태음조위탕과 도담탕을 8주 동안 경구 투여하였을 때 에너지와 포도당 대사에 미치는 영향을 조사하였다. 태음조위탕은 체중과 복부지방에 지방 축적을 나타내는 mesenteric fat과 retroperitoneal fat 양을 감소시키는 경향은 있었지만 통계적으로 유의한 차이를 나타내지는 않았다. 태음조위탕은 대조군에 비해 식이섭취량을 감소시키는 경향을 나타내었다. 반면에 도담탕은 대조군에 비해 체중과 복부지방을 통계적으로 유의하게 감소시켰으며 이것은 식이 섭취량의 감소에 기인하였다. 에너지 소모량은 고지방식이군과 저지방식이군에 차이가 없었고 태음조위탕과 도담탕도 에너지 소모량에 영향을 미치지 않았다. 그러나 에너지원으로 지방을 사용하는 것은 도담탕이 대조군에 비해 높았다. 고지방식이에서는 체내 인슐린 저항성을 나타내는 $HOMA_{IR}$의 값이 높았고, 도담탕은 이것을 통계적으로 유의적으로 낮추었다. 또한, 간에 저장된 글리코겐양도 도담탕을 섭취한 백서에서 가장 많았고, 반면에 간에 저장된 중성 지방 함량은 가장 낮았다. 결론적으로 고지방을 장기간 투여한 암컷 백서에서 체내 에너지와 포도당 대사에 장애가 나타났으며, 태음조위탕과 도담탕이 모두 이러한 장애를 해소하는 경향이 있었으나 도담탕이 효과적으로 에너지와 포도당 대사를 정상화시켰다. 그러므로 비만인 사람에게서 효과적으로 에너지와 포도당 대사를 정상화시킬 수 있는 지에 대한 인체 실험을 하는 것이 필요할 것으로 사료된다.

Keywords

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