DOI QR코드

DOI QR Code

PCR Detection and Molecular Characterization of Pentatrichomonas hominis from Feces of Dogs with Diarrhea in the Republic of Korea

  • Kim, Yun-Ah (Division of Malaria and Parasitic Disease, National Institute of Health, Korea Centers for Disease Control and Prevention) ;
  • Kim, Hye-Youn (Division of Malaria and Parasitic Disease, National Institute of Health, Korea Centers for Disease Control and Prevention) ;
  • Cho, Shin-Hyeong (Division of Malaria and Parasitic Disease, National Institute of Health, Korea Centers for Disease Control and Prevention) ;
  • Cheun, Hyeong-Il (Division of Malaria and Parasitic Disease, National Institute of Health, Korea Centers for Disease Control and Prevention) ;
  • Yu, Jae-Ran (Division of Malaria and Parasitic Disease, National Institute of Health, Korea Centers for Disease Control and Prevention) ;
  • Lee, Sang-Eun (Division of Malaria and Parasitic Disease, National Institute of Health, Korea Centers for Disease Control and Prevention)
  • Received : 2009.07.17
  • Accepted : 2009.11.13
  • Published : 2010.03.15

Abstract

Pentatrichomonas hominis is considered a commensal protozoan in the large intestine of a number of mammalian hosts, such as cats, dogs, and non-human primates. The resulting infections, which can induce diarrhea, have been attributed to opportunistic overgrowth of P. hominis. This study was performed to confirm the P. hominis infection and its molecular characterization from the feces of puppies with diarrhea. Fecal samples were obtained from 14 German shepherd puppies with diarrhea over 1 week (7 females and 7 males, 2-9 months of age) residing on a dog farm in August 2007. Species-specific PCR assay identified P. hominis 18S rRNA genes in 3 of the 14 puppies (1 female and 2 males; 1 aged 2 months and 2 aged 9 months). This phylogenetic analysis established that P. hominis belonged to the 1st clade, which is comprised of Bos taurus and Felines.

Keywords

References

  1. Felleisen RS. Host-parasite interaction in bovine infection with Tritrichomonas foetus. Microbes Infect 1999; 1: 807-816. https://doi.org/10.1016/S1286-4579(99)80083-5
  2. Culberson DE, Pindak FF, Gardner WA, Honiqberq BM. Tritrichomonas mobilensis n. sp. (Zoomastigophorea: trichomonadida) from the Bolivian squirrel monkey Saimiri boliviensis boliviensis. J Protozool 1986; 33: 301-304. https://doi.org/10.1111/j.1550-7408.1986.tb05611.x
  3. Doran DJ. Studies on trichomonads. I. The metabolism of Tritrichomonas foetus and trichomonads from the nasal cavity and cecum of swine. J Protozool 1957; 4: 182-190. https://doi.org/10.1111/j.1550-7408.1957.tb02506.x
  4. Gookin JL, Birkenheuer AJ, St John V, Spector M, Levy MG. Molecular characterization of trichomonads from feces of dogs with diarrhea. J Parasitol 2005; 91: 939-943. https://doi.org/10.1645/GE-474R.1
  5. Kondova I, Simon MA, Klumpp SA, Mackey J, Widmer G, Dominques HG, Persenqiev SP, O'Neil SP. Trichomonad gastritis in Rhesus macaques (Macaca mulatta) infected with simian immunodeficiency virus. Vet Pathol 2005; 42: 19-29. https://doi.org/10.1354/vp.42-1-19
  6. Romatowski J. Pentatrichomonas hominis infection in four kittens.J Am Vet Med Assoc 2000; 216: 1270-1272. https://doi.org/10.2460/javma.2000.216.1270
  7. Guilford WG, Strombeck DR. Gastrointestinal tract infections, parasites, and toxicoses. In Strombeck D.R. ed., Strombeck's Small Animal Gastroenterology. Philadelphia, USA. WB Saunders Co.1996, p 427.
  8. Jergens AE, Willard MD. Diseases of the large intestine. In Etinger SJ, Feldman EC, eds, Textbook of Veterinary Internal Medicine. 5th ed. Philadelphia, USA. WB Saunders Co. 2000, p 1245.
  9. Crucitti T, Abdellati S, Ross DA, Changalucha J, Dyck E, Buve A. Detection of Pentatrichomonas hominis DNA in biological specimens by PCR. Lett Appl Microbiol 2004; 38: 510-516. https://doi.org/10.1111/j.1472-765X.2004.01528.x
  10. Gookin JL, Stauffer SH, Coccaro MR, Marcotte MJ, Levy MG. Optimization of a species-specific polymerase chain reaction assay for identification of Pentatrichomonas hominis in canine fecal specimens. Am J Vet Res 2007; 68: 783-787. https://doi.org/10.2460/ajvr.68.7.783
  11. Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG7. The Clustal X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucl Acids Res 1997; 24: 4876-4882.
  12. Kumar S, Tamura K, Jakobsen IB, Nei M. MEGA3. Integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 2004; 5: 150-163. https://doi.org/10.1093/bib/5.2.150
  13. Dufernez F, Walker RL, Noel C, Caby S, Mantini C, Delgado-Viscogliosi P, Ohkuma M, Kudo T, Capron M, Pierce RJ, Villanueva MR, Viscogliosi E. Morphological and molecular identification of non-Tritrichomonas foetus trichomonad protozoa from the bovine preputial cavity. J Eukaryot Microbiol 2007; 54: 161-168. https://doi.org/10.1111/j.1550-7408.2007.00247.x
  14. Wenrich DH. Morphology of the intestinal trichomonad flagellates in man and of similar forms in monkeys, cats, dogs, and rats. J Morphol 1944; 74: 189-211. https://doi.org/10.1002/jmor.1050740106
  15. Honigberg BM. Evolutionary and systematic relationships in the flagellate order Trichomonadida Kirby. J Protozool 1963; 10: 20-63. https://doi.org/10.1111/j.1550-7408.1963.tb01635.x
  16. Jensen EA, Hammond DM. A morphological study of trichomonads and related flagellates from the bovine digestive tract. J Protozool 1964; 11: 386-394. https://doi.org/10.1111/j.1550-7408.1964.tb01768.x
  17. Bruce, KL. Trichomoniasis in a puppy. Vet Med 1941; 36: 261.
  18. Narayana GS. Intestinal trichomoniasis in a pup: a case report. Indian Vet J 1976; 53: 477.
  19. O'Donell FA. Intestinal trichomoniasis in a dog. Vet Med 1954; 49: 390-391.
  20. Turnwald GH, Barta O, Taylor HW, Kreeger J, Coleman SU, Pourciau SS. Cryptosporidiosis associated with immunosuppression attributable to distemper in a pup. J Am Vet Med Assoc 1988; 191: 79-81.
  21. Simic T. Etude complementaire de l'infection du chien par le trichomonas d'origine humanie, canine et feline. Ann Parasitol 1932; 10: 402-406. https://doi.org/10.1051/parasite/1932105402

Cited by

  1. Update on the Diagnosis and Management of Tritrichomonas foetus Infections in Cats vol.25, pp.3, 2010, https://doi.org/10.1053/j.tcam.2010.08.001
  2. New Hosts of Simplicimonas similis and Trichomitus batrachorum Identified by 18S Ribosomal RNA Gene Sequences vol.2013, pp.None, 2010, https://doi.org/10.1155/2013/831947
  3. Pentatrichomonas hominis: first isolation from the feces of a dog with diarrhea in China vol.113, pp.5, 2010, https://doi.org/10.1007/s00436-014-3825-9
  4. Detection of Pentatrichomonas hominis in dogs using real-time PCR vol.18, pp.4, 2015, https://doi.org/10.1515/pjvs-2015-0100
  5. Prevalence and Molecular Characterization of Intestinal Trichomonads in Pet Dogs in East China vol.54, pp.6, 2016, https://doi.org/10.3347/kjp.2016.54.6.703
  6. Pentatrichomonas hominis infection in two domestic cats with chronic diarrhea vol.4, pp.1, 2010, https://doi.org/10.1177/2055116918774959
  7. High prevalence of Pentatrichomonas hominis infection in gastrointestinal cancer patients vol.12, pp.1, 2010, https://doi.org/10.1186/s13071-019-3684-4
  8. Prevalence of Tetratrichomonas buttreyi and Pentatrichomonas hominis in yellow cattle, dairy cattle, and water buffalo in China vol.119, pp.2, 2010, https://doi.org/10.1007/s00436-019-06550-0
  9. Protozoan genital invasions caused by the representatives of trichomonas and giardia vol.73, pp.2, 2020, https://doi.org/10.36740/wlek202002133
  10. Molecular identification of Pentatrichomonas hominis in animals in central and western Thailand vol.17, pp.1, 2021, https://doi.org/10.1186/s12917-021-02904-y