한국동물위생학회지 (Korean Journal of Veterinary Service)

  • 제33권2호
  • /
  • Pages.129-134
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  • 2010
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  • 3022-7372(pISSN)
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  • 2287-7630(eISSN)
한국동물위생학회 (The Korean Society of Veterinary Service)
권호 표지

경북지방 개 브루셀라병 다두 발생농장의 혈청학적 및 세균학적 조사

Serological and bacteriological study on canine brucellosis in the large kennel farms in Gyeongbuk province

  • 김성국 (경상북도가축위생시험소) ;
  • 서희진 (경상북도가축위생시험소) ;
  • 김순태 (경상북도가축위생시험소) ;
  • 장영술 (경상북도가축위생시험소) ;
  • 조민희 (경상북도가축위생시험소)
  • Kim, Seong-Guk (Gyeongbuk Veterinary Service Laboratory, Eastern Branch Gyeongbuk Veterinary Service Laboratory) ;
  • Seo, Hee-Jin (Gyeongbuk Veterinary Service Laboratory, Eastern Branch Gyeongbuk Veterinary Service Laboratory) ;
  • Kim, Soon-Tae (Gyeongbuk Veterinary Service Laboratory, Eastern Branch Gyeongbuk Veterinary Service Laboratory) ;
  • Jang, Young-Sul (Gyeongbuk Veterinary Service Laboratory, Eastern Branch Gyeongbuk Veterinary Service Laboratory) ;
  • Jo, Min-Hee (Gyeongbuk Veterinary Service Laboratory, Eastern Branch Gyeongbuk Veterinary Service Laboratory)
  • 투고 : 2010.02.19
  • 심사 : 2010.06.11
  • 발행 : 2010.06.30
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초록

Canine brucellosis is a contagious disease of the reproductive tract that cause mainly abortion and infertility in dog. A serological and bacteriological survey was conducted for breeding kennels which were suffered from frequent outbreak of canine brucellosis in Gyeongbuk province in 2009. Among 138 samples, 45 serum samples were sero-positive. Brucella canis was isolated from 30 blood samples of the seropositive cases, and from 2 samples of 62 sero-negatives. The biochemical properties of 32 isolates were characterized with no production of H2S, no fermentation of carbohydrates, hydrolyzation of urea, and development of thionin dye medium. At amplification of BCSP and 16S-rRNA gene using PCR, 711bp and 905bp DNA fragments were detected in agarose. Three tandem repeat pattern was shown in genotyping by Multi-locus VNTR assay (MLVA).

키워드

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