한국임상수의학회지 (Journal of Veterinary Clinics)

  • 제27권4호
  • /
  • Pages.402-406
  • /
  • 2010
  • /
  • 1598-298X(pISSN)
  • /
  • 2384-0749(eISSN)
한국임상수의학회 (The Korean Society of Veterinary Clinics)
권호 표지

젖소에서 소 백혈병 진단법의 효과적 활용

Effective Application of Diagnostics for Bovine Leukemia Virus in Dairy Cattle

  • 심사 : 2010.05.25
  • 발행 : 2010.08.30
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Bovine leukemia virus (BLV) is a delta-retrovirus which causes chronic lymphocytosis in cattle. BLV infections have been divided into two groups such as enzootic bovine leukosis (EBL) and sporadic bovine leukosis (SBL) according to the clinical symptoms in infected cattle. The conventional detection method of BLV was hematological procedure which is determining lymphocytosis in the suspected animals. Recently several sensitive methods were developed to detect antibody to BLV and nucleic acid of the BLV from infected cattle. In this study we have compared the difference of positive rates between agar gel immunodiffusion (AGID) and enzyme linked immunosorbent assay (ELISA) which are using for BLV antibody detection methods. The positive detection rate of ELISA test was 7.4% greater than the positive rate of AGID. The discrepancy of the positive rate between ELISA and AGID were showed in the group of age over one year old to under three year old group. The result from each test agreed very well in the group of over 5 year old cattles. The serological test is very useful method to select the infected cattle for the eradication or control of the disease in the infected herd. But it has a limit by interference of the maternal antibody from the cow of under 6 month old. This study shows that 16.2% of these ages group showed BLV gene positive by polymerase chain reaction (PCR) method. The result suggests that ELISA test need to be used with PCR to clarify misinterpretation of positive animals by antibody response due to the natural infection from maternally derived antibody in calves of under 6 months old.

키워드

참고문헌

  1. 서국현, 이정길, 이채용, 허태영, 강석진, 손동수, 류일선, 안병석, 김남철, 주이석. 우리나라 젖소 및 한우의 Bovine leukemia virus 항체 분포조사. J Vet Clin 2003; 20: 172-176.
  2. 손제영, 김교준. Bovine lymphosarcoma (enzootic bovine leukosis)에 관한 연구: Bovine lymphosarcoma에 관한 대구 및 충남지방 유우군에 대한 혈액학적 조사. 대한수의학회지 1968; 8: 31-38.
  3. 윤순식, 배유찬, 주이석, 위성환, 이경우, 서국현, 박용호, 한홍율. 소 백혈병 바이러스 감염 젖소 말초혈액중 림프구 아군 분포 분석. J bacterial Virol 2005; 35: 165-173.
  4. Ballagi-Pordany A, Belak S. The use of mimics as internal standards to avoid false negatives in diagnostic PCR. Mol Cell Probes 1996; 10: 159-164. https://doi.org/10.1006/mcpr.1996.0022
  5. Bendixen HJ. Bovine enzootic leukosis. Adv Vet Sci Comp Med 1965; 10: 129-204.
  6. Burridge MJ, Thurmond MC, Miller JM, Schmerr MJ, Van Der Maaten MJ. Duration of colostral antibodies to bovine leukemia virus by two serologic tests. Am J Vet Res 1982; 43: 1866-1867.
  7. Choi WP. Survey for antibodies to bovine leukemia virus in dairy and Korean native cattle. Korean J Vet Res 1982; 22: 23-26.
  8. De Giuseppe A, Feliziani F, Rutili D, De Mia GM. Expression of the bovine leukemia virus envelope glycoprotein (gp51) by recombinant baculovirus and its use in an enzyme-linked immunosorbent assay. Clin Diagn Lab Immunol 2004; 11: 147-151.
  9. Enzootic bovine leukosis. In: Terrestrial manual, OIE. 2008: 729-738.
  10. Eaves FW, Molloy JB, Dimmock CK, Eaves LE. A field evaluation of the polymerase chain reaction procedure for the detection of bovine leukaemia virus proviral DNA in cattle. Vet Microbiol 1994; 39: 313-321. https://doi.org/10.1016/0378-1135(94)90167-8
  11. Fechner H, Kurg A, Geue L, Blankenstein P, Mewes G, Ebner D, Beier D. Evaluation of polymerase chain reaction (PCR) application in diagnosis of bovine leukaemia virus (BLV) infection in naturally infected cattle. J Vet Med B 1996; 43: 621-630. https://doi.org/10.1111/j.1439-0450.1996.tb00361.x
  12. Ferrer JF, Abt DA, Bhatt DM, Marshak RR. Studies on the relationship between infection with bovine C-virus, leukemia, and persistent lymphocytosis in cattle. Cancer Research 1974; 34: 893-900.
  13. Jun MH, Chung UI, Lee CK. Seroepizootiological study on bovine leucosis in Korea. Korean J Vet Res 1982; 22: 175-185.
  14. Kajikawa O, Koyama H, Sasaki T, Yohikawa T, Saito H. Studies on enzyme linked immunosolvent assay (ELISA) for detection of antibodies in cattle infected with bovine leukemia virus. Jap J Sci 1983; 45: 347-353. https://doi.org/10.1292/jvms1939.45.347
  15. Klintevall K, Näslund K, Svedlund G, Hajdu L, Linde N, Klingeborn B. Evaluation of an indirect ELISA for the detection of antibodies to bovine leukaemia virus in milk and serum. J Virol Methods 1991; 33: 319-333. https://doi.org/10.1016/0166-0934(91)90032-U
  16. Klintevall K, Ballagi-Pordány A, Näslund K, Belák S. Bovine leukaemia virus: rapid detection of proviral DNA by nested PCR in blood and organs of experimentally infected calves. Vet Microbiol 1994; 42: 191-204. https://doi.org/10.1016/0378-1135(94)90018-3
  17. Knapen K, Kerkhofs P, Thiry E, Mammerickx M. Epidemiological evaluation of a monoclonal ELISA detecting antibodies against bovine leukaemia virus in serum pools. Epidemiol Infect 1994; 113: 563-569. https://doi.org/10.1017/S0950268800068588
  18. Kono Y, Sentsui H, Arai K, Fujigaki A, Enomoto C, Iwasaki H, Ishida H. Serological methods to detect calves infected in utero with bovine leukemia virus. Jap J Vet Sci 1983; 45: 453-461. https://doi.org/10.1292/jvms1939.45.453
  19. Kuckleburg CJ, Chase CC, Nelson EA, Marras SA, Dammen MA, Christopher-Hennings J. Detection of bovine leukemia virus in blood and milk by nested and real-time polymerase chain reactions. J Vet Diagn Invest 2003; 15: 72-76. https://doi.org/10.1177/104063870301500117
  20. Lew AE, Bock RE, Molloy JB, Minchin CM, Robinson SJ, Steer P. Sensitive and specific detection of proviral bovine leukemia virus by 5' Taq nuclease PCR using a 3' minor groove binder fluorogenic probe. J Virol Methods 2004; 115 : 167-175. https://doi.org/10.1016/j.jviromet.2003.09.029
  21. Martin D, Arjona A, Soto I, Barquero N, Viana M, Gómez- Lucía E. Comparative study of PCR as a direct assay and ELISA and AGID as indirect assays for the detection of bovine leukaemia virus. J Vet Med B Infect Dis Vet Public Health 2001; 48: 97-106. https://doi.org/10.1046/j.1439-0450.2001.00424.x
  22. Maruyama K, Fukushima T, Mochizuki S. Cross-reactive antibodies to BLV and HTLV in bovine and human hosts with retrovirus infection. Vet Immunol Immunopahol 1989; 22: 265-274. https://doi.org/10.1016/0165-2427(89)90013-5
  23. Meas S, Seto J, Sugimoto C, Bakhsh M, Riaz M, Sato T, Naeem K, Ohashi K, Onuma M. Infection of bovine immunodeficiency virus and bovine leukemia virus in water buffalo and cattle populations in Pakistan. J Vet Med Sci 2000; 62: 329-331. https://doi.org/10.1292/jvms.62.329
  24. Miller JM, VanDerMaaten MJ. A complement-fixation test for the bovine leukemia (C-type) virus. J Natl Cancer Inst 1974; 53: 1699-1702.
  25. Miller JM, Schmerr MJ, Van Der Maaten MJ. Comparison of four serologic tests for the detection of antibodies to bovine leukemia virus. Am J Vet Res 1981; 42: 5-8.
  26. Olson C. Bovine lymphosarcoma (leukemia). J Am Vet Med Assoc 1974; 165: 630-632.
  27. Onuma M, Olson C, Driscoll DM. Properties of two isolated antigens associated with bovine leukemia virus infection. J Natl Cancer Inst 1976; 57: 571-578.
  28. Perman V, Dirks VA, Fangmann G, Snyder MM, Sorensen DK, Anderson RK, Goltz DJ, Larson VL, Stevens JB. Statistical evaluation of lymphocyte values on Minnesota dairy cattle. Am J Vet Res 1970; 31: 1217-1222.
  29. Ressang AA, Gielkens AL, Quak J, Mastenbroek N. Studies on bovine leukosis. VII. Further experience with an ELISA for the detection of antibodies to bovine leukosis virus. Vet Q 1981; 3: 31-33. https://doi.org/10.1080/01652176.1981.9693791
  30. Simard C, Richardson S, Dixon P, Bélanger C, Maxwell P. Enzyme-linked immunosorbent assay for the diagnosis of bovine leukosis: comparison with the agar gel immunodiffusion test approved by the Canadian Food Inspection Agency. Can J Vet Res 2000; 64: 101-106.
  31. Thurmond MC, Carter RL, Picanso JP, Stralka K. Uppernormal prediction limits of lymphocyte counts for cattle not infected with bovine leukemia virus. Am J Vet Res 1990; 51: 466-470.
  32. Todd D, Adair BM, Wibberley G. An enzyme-linked immunosorbent assay for enzootic bovine leukosis virus antibodies. Vet Rec 1980; 107: 124-126. https://doi.org/10.1136/vr.107.6.124
  33. Usui T, Konnai S, Ohashi K, Onuma M. Interferon-gamma expression associated with suppression of bovine leukemia virus at the early phase of infection in sheep. Vet Immunol Immunopathol 2007; 115: 17-23. https://doi.org/10.1016/j.vetimm.2006.09.011
  34. Ungar-Waron H, Paz R, Brenner J, Yakobson B, Partosh N, Trainin Z. Experimental infection of calves with bovine leukemia virus (BLV): an applicable model of a retroviral infection. Vet Immunol Immunopathol 1999; 67: 195-201. https://doi.org/10.1016/S0165-2427(98)00221-9
  35. Yakobson B, Brenner J, Ungar-Waron H, Trainin Z. Cellular immune response cytokine expression during the initial stage of bovine leukemia virus (BLV) infection determines the disease progression to persistent lymphocytosis. Comp Immunol Microbiol Infect Dis 2000; 23: 197-208. https://doi.org/10.1016/S0147-9571(99)00074-0