Journal of Veterinary Clinics (한국임상수의학회지)

The Korean Society of Veterinary Clinics (한국임상수의학회)
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Effect of Beta-Glucan on the Collagen-Induced Rheumatoid Arthritis

콜라겐유발 관절염에서 폴리칸의 효과

  • Kim, Joo-Wan (Glucan Corporation, Research Institute) ;
  • Cho, Hyung-Rea (Glucan Corporation, Research Institute) ;
  • Kim, Ki-Yung (Glucan Corporation, Research Institute) ;
  • Ku, Sae-Kwang (Department of Anatomy and Histology, College of Oriental medicine, Daegu Haany University) ;
  • Lee, Hyeung-Sik (Department of Clinical Laboratory Science, Daegu Haay University)
  • Accepted : 2010.08.05
  • Published : 2010.08.30
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Abstract

The effect of beta-glucan ($Polycan^{TM}$) derived from Aureobasidium pullulans SM-2001 were observed on, collagen-induced rheumatoid arthritis (RA) in DBA mice. Six week-old male DBA/1J mice were immunized by the intradermal injection of $200\;{\mu}g$ of bovine type II collagen with the equal volume of complete Freund's adjuvant at the tail base on day 1. On day 21, the mice were boosted by the intradermal injection of $200\;{\mu}g$ of bovine type II collagen with incomplete Freund's adjuvant. From the first immunization, mice had been administered $Polycan^{TM}$ (21.25, 42.5 and 85 mg/kg), diclofenac and vehicle once a day for 4 weeks, respectively. Collagen-induced hyperimmunities and arthritis signs were markedly and dose-dependently inhibited by treatment of $Polycan^{TM}$ compared with RA control except for tibial cartilages of $Polycan^{TM}$ 21.25 group. $Polycan^{TM}$ effectively inhibited the histopathological changes of collagen-induced arthritis and hyper-immunities.

본 실험은 Aureobasidium pullulans SM-2001 유래 베타글루칸의 콜라겐 유발 류마티스 관절염에서의 효과를 알아보고자 하였다. 6 주령 DBA/1J 수컷 마우스를 1주일간 적응기간을 거친 후 콜라겐 200 ug를 프로인트 완전면역 보강제와 함께 꼬리부위 피내에 접종하였으며, 21일 후에 콜라겐을 프로인트 불완전면역보강제와 함께 추가접종을 하였다. 베타글루칸 (체중당 21.25, 42.5, 85 mg/kg), 디클로페낙 (체중당 2 mg/kg)을 첫 접종일부터 추가접종 후 1주일간 투여하였다. 관절염 유발군에서는 과증식된 비장(증가된 백색속질)과 연골손상의 증가가 관찰되었다. 이러한 증상은 폴리칸 21.25 mg/kg 투여군의 일부 항목(경골, 연골)을 제외한 모든 폴리칸 투여군에서 용량의존적으로 유의한 효과를 나타내었다. 아우레오바시디움 플루란스 유래 베타글루칸이 콜라겐으로 유발된 류마티스관절염과 과면역을 효과적으로 억제하는 것을 알 수 있었다.

Keywords

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