Korean Journal of Bronchoesophagology (대한기관식도과학회지)

The Korean Bronchoesophagological Society (대한기관식도과학회)
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Biodistribution of Inhaled Titania ($TiO_2$) Nanoparticles in Rats

백서에서 흡인된 티타니아 나노입자의 생체 내 분포에 관한 연구

  • Choi, Se-Hoon (Department of Thoracic and Cardiovascular Surgery, Seoul National University Hospital) ;
  • Park, Kay-Hyun (Department of Thoracic and Cardiovascular Surgery, Seoul National University Bundang Hospital) ;
  • Jheon, San-Hhoon (Department of Thoracic and Cardiovascular Surgery, Seoul National University Bundang Hospital) ;
  • Kim, Joo-Hyun (Department of Thoracic and Cardiovascular Surgery, Seoul National University Hospital) ;
  • Chung, Jin-Haeng (Department of Pathology, Seoul National University Bundang Hospital) ;
  • Cho, So-Hye (Nano-Materials Research Center, Korea Institute of Science and Technology) ;
  • Park, Jong-Ku (Nano-Materials Research Center, Korea Institute of Science and Technology) ;
  • Kim, Tae-Heon (Emergency Medical Center, Chungju St. Mary's Hospital)
  • 최세훈 (서울대학교병원 흉부외과학교실) ;
  • 박계현 (분당서울대학교병원 흉부외과학교실) ;
  • 전상훈 (분당서울대학교병원 흉부외과학교실) ;
  • 김주현 (서울대학교병원 흉부외과학교실) ;
  • 정진행 (분당서울대학교병원 병리학교실) ;
  • 조소혜 (한국과학기술연구원 Nanomaterials 연구센터) ;
  • 박종구 (한국과학기술연구원 Nanomaterials 연구센터) ;
  • 김태헌 (청주성모병원 응급의료센터)
  • Received : 2010.04.13
  • Accepted : 2010.05.20
  • Published : 2010.06.30
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Abstract

Titania nanomaterials are widely used as cosmetics and dyes, however the impacts on human health are uncertain, We investigated the biodistribution of inhaled titania nanoparticles in rats, Methods Eight weeks-old SD rats were intubated and inhaled with 3 mg titania nanoparticles, twice a week, for 2 weeks, After inhalation, the rats were sacrificed and tissues or heart, lung. intestine, brain, and liver were obtained, We investigated the tissues with optical microscope (OM), transmission electron microscope (EM), scanning EM, And to analyze titania concentration of each tissue, we lysed the tissues with radioimmunoprecipitation assay (RlPA) lysis buffer or acid. Results Granulation tissues in lung were confirmed on the optical microscope, however the other organs had no abnormalities in OM images, In EM images, the rats which inhaled titania nanoparticles showed calcium deposition at heart, brain, and intestine, Titania concentration in lung was increased on the inhaled rat sacrificed I month after last exposure. Conclusion Inhaled titania nanoparticles is thought to be deposited and make inflammatory reaction in lung, and the deposition was not efficiently cleared over a month. However inhaled titania nanoparticles may rarely pass through the alveolus-blood barrier and distribute to other organs of the bod.

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