Reproductive and Developmental Biology

The Korean Society of Animal Reproduction (한국동물번식학회)
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Effect of Streptozotocin-Induced Diabetes on Bone and Heart Development in Juvenile Rats

  • Kim, Joo-Heon (Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University) ;
  • Lee, Young-Jeon (Department of Rehabilitation Science in Interdisciplinary PhD Program, Graduate School of Inje University) ;
  • Lee, Sang-Un (Department of Physical Therapy, Aomori University of Health and Welfare) ;
  • Suzuki, Takao (Department of Physical Therapy, Aomori University of Health and Welfare) ;
  • Lee, Sang-Kil (Cardiovascular & Metabolic Disease Center, College of Biomedical Science & Engineering, Inje University) ;
  • Kang, Tae-Young (College of Veterinary Medicine, Jeju National University) ;
  • Hong, Yong-Geun (Department of Rehabilitation Science in Interdisciplinary PhD Program, Graduate School of Inje University)
  • Received : 2010.05.07
  • Accepted : 2010.06.23
  • Published : 2010.06.30
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Abstract

Our objective of current study was to investigate the development of bone and heart in association with diabetes mellitus (DM). DM was induced by administering an intraperitoneal injection of streptozotocin (STZ; 60 mg/kg) to 4-week-old Sprague-Dawley rats. Body weight and blood glucose were monitored, and rats were sacrificed after 2 or 5 weeks. The left ventricle (LV), including the interventricular septum, was weighed, and body weight and tibial bone length were assessed. Young diabetic rats showed reduced growth in terms of tibial length and body weight compared to controls. Moreover, diabetic males showed more significant growth suppression and reduced LV size than diabetic females. Morphometric analysis of tibiae from diabetic rats revealed suppressed bone growth at 2 and 5 weeks, with no difference between genders. STZ-induced diabetes decreased bone growth and retarded pre-pubertal heart development. As a result, diabetes may increase cardiovascular risk factors and lead to eventual heart failure. Therefore, new therapeutic approaches are required for diabetic children exhibiting growth retardation. Heart growth factor, exercise, and cardiopulmonary physical therapy may be required to promote heart development and physiological function.

Keywords

Acknowledgement

Supported by : Rural Development Administration

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