The Korean Journal of Pesticide Science (농약과학회지)

The Korean Society of Pesticide Science (한국농약과학회)
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Biological Control of Garlic Blue Mold using Pantoea agglomerans S59-4

Pantoea agglomerans S59-4를 이용한 마늘 푸른곰팡이병의 생물학적 방제

  • Kim, Yong-Ki (Organic Agriculture Div., Department of Agro-Food Safety, NAAS, RDA) ;
  • Hong, Sung-Jun (Organic Agriculture Div., Department of Agro-Food Safety, NAAS, RDA) ;
  • Jee, Hyung-Jin (Organic Agriculture Div., Department of Agro-Food Safety, NAAS, RDA) ;
  • Park, Jong-Ho (Organic Agriculture Div., Department of Agro-Food Safety, NAAS, RDA) ;
  • Han, Eun-Jung (Organic Agriculture Div., Department of Agro-Food Safety, NAAS, RDA) ;
  • Park, Kyung-Seok (Agricultural Microbiology Div., Department of Agricultural Biology, NAAS, RDA) ;
  • Lee, Sang-Yeob (Agricultural Microbiology Div., Department of Agricultural Biology, NAAS, RDA) ;
  • Lee, Seong-Don (Research Coordination Division, Research Policy Bureau, RDA)
  • 김용기 (국립농업과학원 농산물안전성부 유기농업과) ;
  • 홍성준 (국립농업과학원 농산물안전성부 유기농업과) ;
  • 지형진 (국립농업과학원 농산물안전성부 유기농업과) ;
  • 박종호 (국립농업과학원 농산물안전성부 유기농업과) ;
  • 한은정 (국립농업과학원 농산물안전성부 유기농업과) ;
  • 박경석 (농업생물부 농업미생물과) ;
  • 이상엽 (농업생물부 농업미생물과) ;
  • 이승돈 (농촌진흥청 연구정책국 연구조정과)
  • Received : 2010.04.23
  • Accepted : 2010.05.13
  • Published : 2010.06.30
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Abstract

S59-4 isolate was evaluated as a potential biocontrol agent using in vivo wounded garlic bulb assay. When the spore suspension ($10^5$ spores/$m\ell$) of Penicillium hirsutum was co-inoculated with cell suspension of S59-4 isolate on wounded garlics, the isolate showed high suppressive effect to disease development. The isolate was identified as Pantoea agglomerans S59-4(Pa59-4) through Biolog system. Furthermore, soaking garlic bulbs in the suspension of Pa59-4 significantly reduced garlic decay caused by P. hirsutum. The optimal concentration of Pa59-4 for controlling garlic blue mold was $10^7\sim10^8$ cfu/$m\ell$. And suppressive effect of Pa59-4 on garlic storage decay reduced as inoculation concentration of Penicillium hirsutum increased. In addition in order to investigate population dynamics of Pa59-4 on application site of garlic cloves, two antibiotic markers, pimaricin and vancomycin were selected. Bacterial density of Pa59-4 on the wounded garlic cloves increased continuously both under room temperature condition and low temperature condition until 30days after application of Pa59-4, meanwhile that of Pa59-4 on intact garlic cloves increased until 15days after application of Pa59-4 and thereafter decreased continuously. Two culture media for mass-production of Pa59-4, LB medium and TSB medium, were selected. By-product of bio-fungicide formulated by mixing white carbon and bacterial suspension of Pa59-4 suppressed by 40 to 50% garlic blue mold. Above results suggest that Pa59-4 be a promising control agent against garlic blue mold.

마늘 저장병을 일으키는 푸른곰팡이병의 생물적 방제를 위하여 마늘 근면으로부터 부패억제효과가 우수한 Pantoea agglomerans S59-4(Pa59-4)를 선발하였다. 길항균 Pa59-4의 푸른곰팡이병에 대한 적정처리농도는 $10^7\sim10^8$ cfu/$m\ell$이었으며, Pa59-4의 처리효과는 병원균의 접종농도가 증가됨에 따라 감소되었다. 길항균 Pa59-4의 현탁액에 마늘을 침지 처리할 경우 저장 중 부패를 90%억제하였으며, 종구 분의 처리하여 파종하였을 때에도 무처리구 부패율이 86.7%인데 비해 Pa59-4 처리시 부패율이 23.1%로 부패를 현저히 감소시켰다. 마늘처리부위에서의 길항균의 밀도변동을 알아보기 위하여 항생제 마커로서 pimaricin과 vancomycin을 선발하였고 이들 항생제가 포함된 배지상에서 길항균 Pa59-4의 밀도변동을 저온조건과 상온조건으로 나누어 조사하였을 때 상처를 낸 마늘표면에서는 처리 후 30일까지도 밀도가 계속하여 증가하였으며, 저온 조건하에서는 처리 후 15일까지 증가하다가 그 이후에는 감소되는 것으로 나타났다. 길항균 Pa59-4의 산업화를 위하여 증량 제로 white carbon을 첨가하여 만든 미생물제제를 처리할 경우 저장 중 마늘부패를 40~50% 줄일 수 있었다. 이상의 결과로써 길항균 Pa59-4는 마늘저장 중 부패를 줄일 수 있는 유망한 길항균으로 판단되었다.

Keywords

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