Journal of the Korean Association of Oral and Maxillofacial Surgeons

  • 제36권3호
  • /
  • Pages.161-171
  • /
  • 2010
  • /
  • 2234-7550(pISSN)
  • /
  • 2234-5930(eISSN)
대한구강악안면외과학회 (The Korean Association of Oral and Maxillofacial Surgeons)
권호 표지
DOI QR코드

DOI QR Code

토끼의 하악골에서 $\beta-TCP$ 와 rhBMP-2의 골형성 효과에 대한 형태계측학적 연구

Morphometric analysis on bone formation effect of $\beta-TCP$ and rhBMP-2 in rabbit mandible

  • 김규남 (인하대학교 의과대학 부속병원 구강악안면외과) ;
  • 양정은 (인하대학교 의과대학 부속병원 구강악안면외과) ;
  • 장재원 (인하대학교 의과대학 부속병원 구강악안면외과) ;
  • 사시카라 바라라만 (인하대학교 의과대학 부속병원 구강악안면외과) ;
  • 왕붕 (인하대학교 의과대학 부속병원 구강악안면외과) ;
  • 김일규 (인하대학교 의과대학 부속병원 구강악안면외과)
  • Kim, Kyu-Nam (Department of Oral and Maxillofacial Surgery, Department of Dentistry, College of Medicine, Inha University) ;
  • Yang, Jung-Eun (Department of Oral and Maxillofacial Surgery, Department of Dentistry, College of Medicine, Inha University) ;
  • Jang, Jea-Won (Department of Oral and Maxillofacial Surgery, Department of Dentistry, College of Medicine, Inha University) ;
  • Sasikala, Balaraman (Department of Oral and Maxillofacial Surgery, Department of Dentistry, College of Medicine, Inha University) ;
  • Wang, Beng (Department of Oral and Maxillofacial Surgery, Department of Dentistry, College of Medicine, Inha University) ;
  • Kim, Il-Kyu (Department of Oral and Maxillofacial Surgery, Department of Dentistry, College of Medicine, Inha University)
  • 투고 : 2010.03.10
  • 심사 : 2010.05.04
  • 발행 : 2010.06.30
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Introduction: This study was to assess the effectiveness of new bone formation and regeneration by using a rhBMP-2 and $\beta-TCP$ as a carrier in rabbits’mandible. Materials and Methods: The mandibles of 36 rabbits were exposed and cortical bone was penetrated for this study. The experimental subjects were divided into 3 groups each 12 rabbits ; control group, experimental group 1, and experimental group 2. Control group had the defect itself without any treatment, in the experimental group 1, $\beta-TCP$P only was grafted, and in the experimental group 2, rhBMP-2 soaked in $\beta-TCP$ was grafted. The rabbits were sacrificed after 1, 2, 3, 4, 6, and 8weeks, and new bone formation area was examined and measured for bone quantitative and qualitative analysis with light, fluorescent and polarized microscopy. Results: In the experimental group 1, new bone formation from the adjacent host bone was made by osteoconduction, and in the experimental group 2, direct new bone formation by osteoinduction of rhBMP-2 as well as new bone formation by osteoconduction of $\beta-TCP$ were observed. Conclusion: rhBMP-2 of experimental group 2 is very effective in the bone formation in early 2weeks and bone remodelling from 3weeks.

키워드

참고문헌

  1. Proussaefs P, Lozada J. The use of intraorally harvested autogenous block grafts for vertical alveolar ridge augmentation: a human study. Int J Periodontics Restorative Dent 2005;25:351-63.
  2. Springfield D. Aograft reconstructions. Orthop Clin North Am 1996;27:483-92.
  3. Rummelhart JM, Mellonig JT, Gray JL, Towle HJ. A comparison of freeze-dried bone allograft and demineralized freeze-dried bone allograft in human periodontal osseous defects. J Periodontol 1989;60:655-63. https://doi.org/10.1902/jop.1989.60.12.655
  4. Sculean A, Stavropoulos A, Windisch P, Keglevich T, Karring T, Gera I.Healing of human intrabony defects following regenerative periodontal therapy with a bovine derived xenograft and guided tissue regeneration. Clin Oral Investig 2004;8:70-4.
  5. Hallman M, Nordin T. Sinus floor augmentation with bovine hydroxyapatite mixed with fibrin glue and later placement of nonsubmerged implants: a retrospective study in 50 patients. Int J Oral Maxillofac Implants 2004;19:222-7.
  6. Proussaefs P, Lozada J, Rohrer MD. A clinical and histologic evaluation of a block onlay graft in conjunction with autogenous particulate and inorganic bovine mineral (Bio-Oss): a case report. Int J Periodontics Restorative Dent 2002;22:567-73.
  7. Taba M Jr, Jin Q, Sugai JV, Giannobile WV. Current concepts in periodontal bioengineering. Orthod Craniofac Res 2005;8:292- 302. https://doi.org/10.1111/j.1601-6343.2005.00352.x
  8. Dunn CA, Jin Q, Taba M Jr, Franceschi RT, Bruce Rutherford R, Giannobile WV. BMP gene delivery for alveolar bone engineering at dental implants defects. Mol Ther 2005;11:294-9. https://doi.org/10.1016/j.ymthe.2004.10.005
  9. Urist MR. Bone: formation by autoinduction. Science 1965;150: 893-9. https://doi.org/10.1126/science.150.3698.893
  10. Wozney JM, Rosen V, Celeste AJ, Mitsock LM, Whitters MJ, Kriz RW, et al. Novel regulators of bone formation: molecular clones and activities. Science 1988;242:1528-34 https://doi.org/10.1126/science.3201241
  11. Hou LT, Liu CM, Liu BY, Chang PC, Chen MH, Ho MH, et al. Tissue engineering bone formation in novel recombinant human bone morphogenic protein 2-atelocollagen composite scaffolds. J Periodontol 2007;78:335-43. https://doi.org/10.1902/jop.2007.060106
  12. Wozney JM, Rosen V. Bone morphogenetic protein and bone morphogenetic protein gene family in bone formation and repair. Clin Orthop 1998;346:26-37.
  13. Yamaguchi A, Komori T, Suda T. Regulation of osteoblast differentiation mediated by bone morphogenetic proteins, hedgehogs, and Cbfa1. Endocr Rev 2000;21:393-411. https://doi.org/10.1210/er.21.4.393
  14. Geiger M, Li RH, Friess W. Collagen sponges for bone regeneration with rhBMP-2. Adv Drug Deliv Rev 2003;55:1613-29. https://doi.org/10.1016/j.addr.2003.08.010
  15. Saito N, Taka K. New synthetic biodegradable polymers as BMP carriers for bone tissue engineering. Biomaterials 2003;24:2287- 93. https://doi.org/10.1016/S0142-9612(03)00040-1
  16. Aldinger G, Herr G, Kusswetter W, Reis HJ, Thielemann FW, Holz U. Bone morphogenetic protein: a review. Int Orthop 1991; 15:169-77.
  17. Moskow BS, Lubarr A. Histological assessment of human periodontal defect after durapatite ceramic implant. Report of a case. J Periodontol 1983;54:455-62. https://doi.org/10.1902/jop.1983.54.8.455
  18. Saffar JL, Colombier ML, Detienville R. Bone formation in tricalcium phosphate-filled periodontal intrabony lesions. Histological observations in humans. J Periodontol 1990;61:209- 16. https://doi.org/10.1902/jop.1990.61.4.209
  19. Winn SR, Uludag H, Hollinger JO. Carrier systems for bone morphogenetic proteins. Clin Orthop 1999;(367 Suppl):S95-106.
  20. Kim HJ, Choi SM, Ku Y, Rhyu IC, Chung CP, Han SB, et al. The effect of rhBMP-2 on the osteoblastic differentiation of human periodontal ligament cells and gingival fibroblasts in vitro. J Periodontal Implant Sci 2002;32:389-402.
  21. Granjeiro JM, Oliveira RC, Bustos-Valenzuela JC, Sogayar MC, Taga R. Bone morphogenetic proteins: from structure to clinical use. Braz J Med Biol Res 2005;38:1463-73. https://doi.org/10.1590/S0100-879X2005001000003
  22. Barboza EP, Duarte ME, Geola′s L, Sorensen RG, Riedel GE, Wikesjo UM.Ridge augmentation following implantation of recombinant human bone morphogenetic protein-2 in the dog. J Periodontol 2000;71:488-96. https://doi.org/10.1902/jop.2000.71.3.488
  23. Bessho K, Carnes DL, Cavin R, Chen HY, Ong JL. BMP stimulation of bone response adjacent to titanium implants in vivo. Clin Oral Implants Res 1999;10:212-8. https://doi.org/10.1034/j.1600-0501.1999.100304.x
  24. Ren WH, Yang LJ, Dong SZ. Induction of reparative dentin formation in dogs with combined recombinant human bone morphogenetic protein 2 and fibrin sealant. Chin J Dent Res 1999;2:21-4.
  25. Jepsen S, Albers HK, Fleiner B, Tucker M, Rueger D. Recombinant human osteogenic protein-1 induces dentin formation: an experimental study in miniature swine. J Endod 1997;23: 378-82. https://doi.org/10.1016/S0099-2399(97)80187-2
  26. Sigurdsson TJ, Fu E, Tatakis DN, Rohrer MD, Wikesjo UM. Bone morphogenetic protein-2 for peri-implant bone regeneration and osseointegration. Clin Oral Implants Res 1997;8:367-74. https://doi.org/10.1034/j.1600-0501.1997.080503.x
  27. Kato M, Toyoda H, Namikawa T, Hoshino M, Terai H, Miyamoto S, et al. Optimized use of a biodegradable polymer as a carrier material for the local delivery of recombinant human bone morphogenetic protein-2 (rhBMP-2). Biomaterials 2006;27: 2035-41. https://doi.org/10.1016/j.biomaterials.2005.10.007
  28. Tatakis DN, Koh A, Jin L, Wozney JM, Rohrer MD, Wikesjo ¨ UM. Peri-implant bone regeneration using recombinant human bone morphogenetic protein-2 in a canine model: a dose-response study. J Periodontal Res 2002;37:93-100. https://doi.org/10.1034/j.1600-0765.2002.00021.x
  29. Jung RE, Weber FE, Thoma DS, Ehrbar M, Cochran DL, H¨a - mmerle CH. Bone morphogenetic protein-2 enhances bone formation when delivered by a synthetic matrix containing hydroxyapatite/ tricalciumphosphate. Clin Oral Implants Res 2008;19: 188-95. https://doi.org/10.1111/j.1600-0501.2007.01431.x
  30. Yuan H, de Bruijn JD, Zhang X, van Blitterswijk CA, de Groot K. Use of an osteoinductive biomaterial as a bone morphogenetic protein carrier. J Mater Sci Mater Med 2001;12:761-6. https://doi.org/10.1023/A:1013957431372
  31. Liu Y, Huse RO, de Groot K, Buser D, Hunziker EB. Delivery mode and efficacy of BMP-2 in association with implants. J Dent Res 2007;86:84-9. https://doi.org/10.1177/154405910708600114
  32. Bouxsein ML, Turek TJ, Blake CA, D'Augusta D, Li X, Stevens M, et al.Recombinant human bone morphogenetic protein-2 accelerates healing in a rabbit ulnar osteotomy model. J Bone Joint Surg Am 2001 83-A:1219-30. https://doi.org/10.2106/00004623-200108000-00012
  33. Lee YM, Park YJ, Lee SJ, Ku Y, Han SB, Choi SM, et al. Tissue engineered bone formation using chitosan/tricalcium phosphate sponges. J Periodontol 2000;71:410-7. https://doi.org/10.1902/jop.2000.71.3.410
  34. Szabo G, Suba Z, Hrabak K, Barabas J, Nemeth Z. Autogenous bone versus beta-tricalcium phosphate graft alone for bilateral sinus elevations (2-and 3-dimensional computed tomographic, histologic, and histomorp hometric evaluations): preliminary results. Int J Oral Maxillofac Implants 2001;16:681-92.
  35. Lu J, Descamps M, Dejou J, Koubi G, Hardouin P, Lemaitre J, et al. The biodegradation mechanism of calcium phosphate biomaterials in bone. J Biomed Mater Res 2002;63:408-12. https://doi.org/10.1002/jbm.10259
  36. Jensen SS, Broggini N, Hjrting-Hansen E, Schenk R, Buser D. Bone healing and graft resorption of autograft, anorganic bovine bone and beta-TCP. A histologic and histomorphometric study in the mandibles of minipigs. Clin Oral Implants Res 2006;17:237- 43. https://doi.org/10.1111/j.1600-0501.2005.01257.x

피인용 문헌

  1. A comparative study of the effectiveness of sinus bone grafting with recombinant human bone morphogenetic protein 2-coated tricalcium phosphate and platelet-rich fibrin-mixed tricalcium phosphate in r vol.113, pp.5, 2012, https://doi.org/10.1016/j.tripleo.2011.04.029
  2. Pulsed Electromagnetic Fields Enhance Bone Morphogenetic Protein-2 Dependent-Bone Regeneration vol.21, pp.19, 2010, https://doi.org/10.1089/ten.tea.2015.0032
  3. Osteogenic Effect of a Biodegradable BMP-2 Hydrogel Injected into a Cannulated Mg Screw vol.6, pp.11, 2010, https://doi.org/10.1021/acsbiomaterials.0c00709