Journal of Embryo Transfer (한국수정란이식학회지)

The Korean Society of Embryo Transfer (한국수정란이식학회)
권호 표지

Gene Expression of the In Vitro Fertilized or Somatic Cell Nuclear Transfer Embryos Cultured in Medium Supplemented with Different Proteins or Energy Substrates

  • Jang, Goo (Department of Theriogenology & Biotechnology, College of Veterinary Medicine, Seoul National University) ;
  • Ko, Kyeong-Hee (Department of Theriogenology & Biotechnology, College of Veterinary Medicine, Seoul National University) ;
  • Jeon, Hyun-Yong (Department of Theriogenology & Biotechnology, College of Veterinary Medicine, Seoul National University) ;
  • Lee, Byeong-Chun (Department of Theriogenology & Biotechnology, College of Veterinary Medicine, Seoul National University)
  • Received : 2010.05.16
  • Accepted : 2010.05.27
  • Published : 2010.06.30
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Abstract

Several cloned animals have been produced using somatic cell nuclear transfer (SCNT) and have interested in producing the transgenic cloned animals to date. But still its efficiency was low due to a number of reasons, such as sub-optimal culture condition, aberrant gene expression and nuclear reprogramming. The purpose of this study was to analyze gene expression pattern in in vitro fertilized (IVF) or SCNT pre-implantation embryos. IVF- or SCNT-embryos were cultured in media supplemented with different proteins (FBS and BSA) or energy sources (glucose or fructose). Blastocysts from IVF or SCNT were analyzed using semi-quantitative RT-PCR in terms of developmentor metabolic-related genes. Culture medium supplemented different proteins or energy sources had affected on the expression of developmental or metabolic genes in the SCNT blastocysts.

Keywords

References

  1. Barnett DK and Bavister BD. 1996. What is the relationship between the metabolism of preimplantation embryos and their developmental competence. Mol. Reprod. Dev. 43: 105-133. https://doi.org/10.1002/(SICI)1098-2795(199601)43:1<105::AID-MRD13>3.0.CO;2-4
  2. Barnett DK, Clayton MK, Kimura J and Bavister BD. 1997. Glucose and phosphate toxicity in hamster preimplantation embryos involves disruption of cellular organization, including distribution of active mitochondria. Mol. Reprod. Dev. 48:227-237. https://doi.org/10.1002/(SICI)1098-2795(199710)48:2<227::AID-MRD10>3.0.CO;2-V
  3. Bertolini M, Beam SW, Shim H, Bertolini LR, Moyer AL, Famula TR and Anderson G.B. 2002. Growth, development, and gene expression by in vivo- and in vitro-produced day 7 and 16 bovine embryos. Mol. Reprod. Dev. 63:318-328. https://doi.org/10.1002/mrd.90015
  4. Bhuiyan MM, Cho JK, Jang G, Park ES, Kang SK, Lee BC and Hwang WS. 2004. Effect of protein supplementation in potassium simplex optimization medium on preimplantation development of bovine non-transgenic and transgenic cloned embryos. Theriogenology 62:1403-1416. https://doi.org/10.1016/j.theriogenology.2004.02.006
  5. Blondin P, Farin PW, Crosier AE, Alexander JE and Farin CE. 2000. In vitro production of embryos alters levels of insulin- like growth factor-II messenger ribonucleic acid in bovine fetuses 63 days after transfer. Biol. Reprod. 62:384-389. https://doi.org/10.1095/biolreprod62.2.384
  6. Brackett BG, Bousquet D, Boice ML, Donawick WJ, Evans JF and Dressel MA. 1982. Normal development following in vitro fertilization in the cow. Biol. Reprod. 27:147-158. https://doi.org/10.1095/biolreprod27.1.147
  7. Brophy B, Smolenski G, Wheeler T, Wells D, L'Huillier P and Laible G. 2003. Cloned transgenic cattle produce milk with higher levels of beta-casein and kappa-casein. Nat. Biotechnol. 21:157-162. https://doi.org/10.1038/nbt783
  8. Chatot CL, Ziomek CA, Bavister BD, Lewis JL and Torres I. 1989. An improved culture medium supports development of random-bred l-cell mouse embryos in vitro. J. Reprod. Fertil. 86:679-688. https://doi.org/10.1530/jrf.0.0860679
  9. Cibelli JB, Stice SL, Golueke PJ, Kane JJ, Jerry J, Blackwell C, Ponce de Leon FA and Robl JM. 1998. Cloned transgenic calves produced from nonquiescent fetal fibroblasts. Science 280:1256-1258. https://doi.org/10.1126/science.280.5367.1256
  10. Conaghan J, Handyside AH, Winston RM and Leese HJ. 1993. Effects of pyruvate and glucose on the development of human preimplantation embryos in vitro. J. Reprod. Fertil. 99:87-95. https://doi.org/10.1530/jrf.0.0990087
  11. Hardy K. 1997. Cell death in the mammalian blastocyst. Mol. Hum. Reprod. 3:919-925. https://doi.org/10.1093/molehr/3.10.919
  12. Jurisicova A, Latham KE, Casper RF, Casper RF and Varmuza SL. 1998. Expression and regulation of genes associated with cell death during murine preimplantation embryo development. Mol. Reprod. Dev. 51:243-253. https://doi.org/10.1002/(SICI)1098-2795(199811)51:3<243::AID-MRD3>3.0.CO;2-P
  13. Kim JH, Niwa K, Lim JM and Okuda K. 1993. Effects of phosphate, energy substrates, and amino acids on development of in vitro-matured, in vitro-fertilized bovine oocytes in a chemically defined, protein-free culture medium. Biol. Reprod. 48:1320-1325. https://doi.org/10.1095/biolreprod48.6.1320
  14. Kishi J, Noda Y, Narimoto K, Umaoka Y and Mori T. 1991. Block to development in cultured rat I-cell embryos is overcome using medium HECM-1. Hum. Reprod. 6:1445-1448. https://doi.org/10.1093/oxfordjournals.humrep.a137286
  15. Kwun J, Chang K, Lim J, Lee E, Lee B, Kang S and Hwang W. 2003. Effects of exogenous hexoses on bovine in vitro fertilized and cloned embryo development: Improved blastocyst formation after glucose replacement with fructose in a serum-free culture medium. Mol. Reprod. Dev. 65:167-174. https://doi.org/10.1002/mrd.10309
  16. Langenbach R, Morham SG, Tiano HF, Loftin CD, Ghanayem BI, Chulada PC, Mahler JF, Lee CA, Goulding EH, Kluckman KD, Kim HS and Smithies O. 1995. Prostaglandin synthase 1 gene disruption in mice reduces arachidonic acid-induced inflammation and indomethacin-induced gastric ulceration. Cell 83:483-492. https://doi.org/10.1016/0092-8674(95)90126-4
  17. Lawitts JA and Biggers JD. 1991. Overcoming the 2-cell block by modifying standard components in a mouse embryo culture medium. Biol. Reprod. 45:245-251. https://doi.org/10.1095/biolreprod45.2.245
  18. Lim H, Paria BC, Das SK, Dinchuk JE, Langenbach R, Trzaskos JM and Dey SK. 1997. Multiple female reproductive failures in cyclooxygenase 2-deficient mice. Cell 91:197-208. https://doi.org/10.1016/S0092-8674(00)80402-X
  19. Lonergan P, Rizos D, Gutierrez-Adan A, Fair T and Boland MP. 2003. Oocyte and embryo quality: Effect of origin, culture conditions and gene expression patterns. Reprod. Domest. Anim. 38:259-267. https://doi.org/10.1046/j.1439-0531.2003.00437.x
  20. Machaty Z, Day BN and Prather RS. 1998. Development of early porcine embryos in vitro and in vivo. Biol. Reprod. 59:451-455. https://doi.org/10.1095/biolreprod59.2.451
  21. Matsuyama K, Miyakoshi H and Fukui Y. 1993. Effect of glucose levels during the in vitro culture in synthetic oviduct fluid medium on in vitro development of bovine oocytes matured and fertilization in vitro. Theriogenology 40:595-605. https://doi.org/10.1016/0093-691X(93)90412-X
  22. Matwee C, Betts DH and King WA. 2000. Apoptosis in the early bovine embryo. Zygote 8:57-68. https://doi.org/10.1017/S0967199400000836
  23. Menezo YJ and Herubel F. 2002. Mouse and bovine models for human IVF. Reprod. Biomed. Online 4:170-175. https://doi.org/10.1016/S1472-6483(10)61936-0
  24. Miyoshi K, Funahashi H, Okuda K and Niwa K. 1994. Development of rat one-cell embryos in a chemically defined medium: Effects of glucose, phosphate and osmolarity. J. Reprod. Fertil. 100:21-26. https://doi.org/10.1530/jrf.0.1000021
  25. Moore T. 2001. Genetic conflict, genomic imprinting and establishment of the epigenotype in relation to growth. Reproduction 122:185-193. https://doi.org/10.1530/rep.0.1220185
  26. Nedambale TL, Du F, Yang X and Tian XC. 2006. Higher survival rate of vitrified and thawed in vitro produced bovine blastocysts following culture in defined medium supplemented with beta-mercaptoethanol. Anim. Reprod. Sci. 93: 61-75. https://doi.org/10.1016/j.anireprosci.2005.06.027
  27. Niemann Hand Wrenzycki C. 2000. Alterations of expression of developmentally important genes in preimplantation bovine embryos by in vitro culture conditions: Implications for subsequent development. Theriogenology 53:21-34. https://doi.org/10.1016/S0093-691X(99)00237-X
  28. Niemann H, Wrenzycki C, Lucas-Hahn A, Brambrink T, Kues WA and Carnwath JW. 2002. Gene expression patterns in bovine in vitro-produced and nuclear transfer-derived embryos and their implications for early development. Cloning Stem Cells 4:29-38. https://doi.org/10.1089/153623002753632020
  29. Oyamada T and Fukui Y. 2004. Oxygen tension and medium supplements for in vitro maturation of bovine oocytes cultured individually in a chemically defined medium. J. Reprod. Dev. 50:107-117. https://doi.org/10.1262/jrd.50.107
  30. Parrish JJ, Susko-Parrish JL, Leibfried-Rutledge ML, Crister ES, Eyestone WH, and First NL. 1986. Bovine in vitro fertilization with frozen-thawed semen. Theriogenology 25:591-600. https://doi.org/10.1016/0093-691X(86)90143-3
  31. Pierce GB, Lewellyn AL and Parchment RE. 1989. Mechanism of programmed cell death in the blastocyst. Proc. Natl. Acad. Sci. U.S.A. 86:3654-3658.
  32. Quinn P. 1995. Enhanced results in mouse and human embryo culture using a modified human tubal fluid medium lacking glucose and phosphate. J. Assist. Reprod. Genet. 12:97-105. https://doi.org/10.1007/BF02211377
  33. Rosenkrans CFJ, and First NL. 1991. Culture of bovine zygotes to the blastocyst stage: Effect of amino acids and vitamins. Theriogenology. 35:266. https://doi.org/10.1016/0093-691X(91)90242-6
  34. Schini SA and Bavister BD. 1988. Two-cell block to development of cultured hamster embryos is caused by phosphate and glucose. BioI. Reprod. 39:1183-1192. https://doi.org/10.1095/biolreprod39.5.1183
  35. Scott L and Whittingham DG. 1996. Influence of genetic background and media components on the development of mouse embryos in vitro. Mol. Reprod. Dev. 43:336-346. https://doi.org/10.1002/(SICI)1098-2795(199603)43:3<336::AID-MRD8>3.0.CO;2-R
  36. Shin SJ, Lee BC, Park JI, Lim JM, and Hwang WS. 2001. A separate procedure of fusion and activation in an ear fibroblast nuclear transfer program improves preimplantation development of bovine reconstituted oocytes. Theriogenology 55:1697-1704. https://doi.org/10.1016/S0093-691X(01)00513-1
  37. Szafranska B, Xie S, Green J. and Roberts RM. 1995. Porcine pregnancy-associated glycoproteins: New members of the aspartic proteinase gene family expressed in trophectoderm. BioI. Reprod. 53:21-28. https://doi.org/10.1095/biolreprod53.1.21
  38. Thompson JG, Simpson AC, Pugh PA and Tervit HR. 1992. Requirement for glucose during in vitro culture of sheep preimplantation embryos. Mol. Reprod. Dev. 31:253-257. https://doi.org/10.1002/mrd.1080310405
  39. .Thompson JG. 2000. In vitro culture and embryo metabolism of cattle and sheep embryos - a decade of achievement. Anim. Reprod. Sci. 60-61:263-275. https://doi.org/10.1016/S0378-4320(00)00096-8
  40. Walker SK, Heard TM and Seamark RF. 1992. In vitro culture of sheep embryos without co-culture: successes and perspectives. Theriogenology 37:111-126. https://doi.org/10.1016/0093-691X(92)90250-U
  41. Wrenzycki C, Lucas-Hahn A, Herrmann D, Lemme E, Korsawe K and Niemann H. 2002. In vitro production and nuclear transfer affect dosage compensation of the X-linked gene transcripts G6PD, PGK, and Xist in preimplantation bovine embryos. BioI. Reprod. 66:127-134. https://doi.org/10.1095/biolreprod66.1.127
  42. Wrenzycki C, Wells D, Herrmann D, Miller A, Oliver J, Tervit R and Niemann H. 2001b. Nuclear transfer protocol affects messenger RNA expression patterns in cloned bovine blastocysts. BioI. Reprod. 65:309-317. https://doi.org/10.1095/biolreprod65.1.309
  43. Young LE, Fernandes K, McEvoy TG, Butterwith SC, Gutierrez CG, Carolan C, Broadbent PJ, Robinson JJ, Wilmut I and Sinclair KD. 2001. Epigenetic change in IGF2R is associated with fetal overgrowth after sheep embryo culture. Nat. Genet. 27:153-154. https://doi.org/10.1038/84769