Journal of Embryo Transfer (한국수정란이식학회지)

The Korean Society of Embryo Transfer (한국수정란이식학회)
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Comparison of Mechanical and Enzymatic Methods for the Isolation of Bovine Ovarian Follicles

  • Lim, Hyun-Joo (Dairy Science Division, National Institute of Animal Science, RDA) ;
  • Kim, Dong-Hoon (Dairy Science Division, National Institute of Animal Science, RDA) ;
  • Im, Gi-Sun (Dairy Science Division, National Institute of Animal Science, RDA) ;
  • Lim, Jeong-Mook (Research Institute for Agriculture and Life Sciences, Department of Animal and Food Biotechnology, Seoul National University)
  • Received : 2010.04.13
  • Accepted : 2010.04.28
  • Published : 2010.06.30
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Abstract

The isolation of preantral follicles from the ovaries of bovine was performed under mechanical and enzymatic methods. A significant increase in the total number of follicles retrieved was detected when tissue chopper was used. Micro-dissection could supply good quality, larger sized follicles (400 to $700{\mu}m$) but with the lowest yield ($9.0{\pm}1.0$). The isolated preantral and early antral follicles were cultured for 14 days. Follicles isolated by the mechanical method had a greater growth during a culture period than follicles collected enzymatically. Morphologically normal bovine oocytes from early antral follicles after 14 days culture were 59.6% after culture and after 24 h of maturation culture, 12.9% of in vitro-grown oocytes reached the second metaphase. In conclusion, this study showed that mechanical method can be used effectively to isolate intact preantral follicles from bovine ovaries.

Keywords

References

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