Parasites, Hosts and Diseases

The Korea Society for Parasitology and Tropical Medicine (대한기생충학·열대의학회)
권호 표지
DOI QR코드

DOI QR Code

Production of Polyclonal Antibodies against the Tegument of Sparganum (Plerocercoid of Spirometra mansoni) and Its Immunolocalization

  • Yang, Hyun-Jong (Department of Parasitology and Ewha Medical Research Center, Ewha Womans University, School of Medicine)
  • Received : 2009.11.25
  • Accepted : 2010.03.17
  • Published : 2010.06.30
Download PDF
⟨ Previous Next ⟩

Abstract

In a previous study, the author developed a method for separation of the tegument of spargana (plerocercoids of Spirometra mansoni) from the parenchyme using urea. The present study, as a next step, was performed to evaluate which molecules are present in the outer tegument. Two major proteins, 180 and 200 kDa, are present in the tegument and we could make polyclonal antibodies against these molecules. Their immunolocalization was processed and the outermost layer of the spargana showed strong positive staining. Conclusively, we could confirm that the 180 and 200 kDa molecules might be tightly bound membrane proteins in the tegument of spargana.

Keywords

Acknowledgement

Supported by : Ewha Womans University

References

  1. Chang KH, Chi JG, Cho SY, Han MH, Han DH, Han MC. Cerebral sparganosis: analysis of 34 cases with emphasis on CT features. Neuroradiology 1992; 34: 1-8. https://doi.org/10.1007/BF00588423
  2. Cho SY, Bae JH, Seo BS, Lee SH. Some aspects of human sparganosis in Korea. Korean J Parasitol 1975; 13: 60-77. https://doi.org/10.3347/kjp.1975.13.1.60
  3. Wilson RA, Curwen RS, Braschi S, Hall SL, Coulson PS, Ashton PD. From genomes to vaccines via the proteome. Mem Inst Oswaldo Cruz 2004; 99: 45-50. https://doi.org/10.1590/S0074-02762004000100008
  4. Kim LS, Kong Y, Kang SY, Cho SY. Immunohistochemical localization of 36 and 29 kDa proteins in sparganum. Korean J Parasitol 1992; 30: 25-31. https://doi.org/10.3347/kjp.1992.30.1.25
  5. Ohnishi Y, Takakura Y, Yoshimura H, Tsubota N. Antibody production in experimental sparganosis of rabbits: agglutination and indirect fluorescent antibody techniques. Jpn J Parasitol 1986; 35: 25-33.
  6. Kim CH, Choi WS. Immunohistochemical observation on the antigens inducing IgG antibodies and IgM against sparganum. Korean J Parasitol 1991; 29: 339-353. https://doi.org/10.3347/kjp.1991.29.4.339
  7. Yang HJ. Separation of the syncytia l layer of spargana using urea.Korean J Parasitol 2009; 47: 69-71. https://doi.org/10.3347/kjp.2009.47.1.69
  8. Mills GL, Coley SC, Williams JF. Lipid and protein composition of the surface tegument from larvae of Taenia taeniformis. J Parasitol 1984; 70: 197-207. https://doi.org/10.2307/3281862
  9. Kim SI, Cho SY. Protein composition and antigenicity of the tegument from Paragonimus westermani. Korean J Parasitol 1993; 31: 269-276. https://doi.org/10.3347/kjp.1993.31.3.269
  10. Kusel JR. Protein composition and protein synthesis in the surface membranes of Schistosoma mansoni. Parasitology 1972; 65: 55-69. https://doi.org/10.1017/S0031182000044231
  11. Oak JA, Cain GD, Mower DA, Raj RK. Disruption and removal of the tegument from Schistosoma mansoni with Triton X-100. J Parasitol 1981; 67: 761-775. https://doi.org/10.2307/3280697
  12. van Balkom BW, van Gestel RA, Brouwers JFHM, Krijgsveld J, Tielens AGM, Heck AJ, van Hellemond JJ. Mass spectrometric analysis of the Schistosoma mansoni tegumental sub-proteome. J Proteome Res 2005; 4: 958-966. https://doi.org/10.1021/pr050036w
  13. Jones MK, Gobert GN, Zhang L, Sunderland P, McManus DP. The cytoskeleton and motor proteins of human schistosomes and their roles in surface maintenance and host-parasite interactions. Bioessays 2004; 26: 752-765. https://doi.org/10.1002/bies.20058
  14. Chung YB, Kong Y, Yang HJ, Cho SY. IgG antibody responses in early experimental sparganosis and IgG subclass responses in human sparganosis. Korean J Parasitol 2000; 38: 145-150. https://doi.org/10.3347/kjp.2000.38.3.145

Cited by

  1. Identification of an enolase gene and its physiological role in Spirometra mansoni vol.120, pp.6, 2010, https://doi.org/10.1007/s00436-021-07175-y